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[191717_s_at]

Description C33H5.18 /REP_DB=WormBase Gene ID /WP=CE04161 /CHR=4 /FEA=Sanger Annotation /DEF=CDP-diacylglycerol synthase (ST.LOUIS) SW:P53439 protein_id:AAA82275.1
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 16      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 0      6grp_total > 17      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1349
1 2 3 4 range
A 2299.6
(3)
P 		1132.2
(-2.8)
P 		1101.6
(-3)
P 		1278.3
(-2.1)
P 		1198
B 2402.6
(3)
P 		1083
(-2.9)
P 		1053.6
(-3)
P 		1314
(-1.8)
P 		1349
C 2092.5
(3)
P 		1150.4
(-2.8)
P 		1121.6
(-3)
P 		1265.6
(-2.1)
P 		971
avr 2264.9
(3) 		1121.9
(-2.8) 		1092.3
(-3) 		1286
(-2) 		1173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 876
1 2 3 4 5 6 range
A 1918.6
(3)
P 		1640
(0.3)
P 		1358
(-2.4)
P 		1338.7
(-2.6)
P 		1298.6
(-3)
P 		1457.2
(-1.5)
P 		620
B 2027.5
(3)
P 		1672.4
(0.4)
P 		1238.3
(-2.9)
P 		1538
(-0.6)
P 		1219.5
(-3)
P 		1275.3
(-2.6)
P 		808
C 1949.6
(3)
P 		1585.9
(0.3)
P 		1480.4
(-0.5)
P 		1151.6
(-3)
P 		1321.2
(-1.7)
P 		1289.3
(-2)
P 		798
avr 1965.2
(3) 		1632.8
(0.1) 		1358.9
(-2.3) 		1342.8
(-2.4) 		1279.8
(-3) 		1340.6
(-2.5) 		685
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 650
1 2 3 4 range
A 837.3
(3)
P 		245.8
(-2.5)
P 		288
(-2.1)
P 		187.1
(-3)
P 		650
B 766.2
(3)
P 		267.5
(-2.7)
P 		244.1
(-3)
P 		280
(-2.6)
P 		522
C 692.7
(3)
P 		330.7
(-1.5)
P 		390.9
(-0.8)
P 		213.4
(-3)
P 		479
avr 765.4
(3) 		281.3
(-2.4) 		307.7
(-2.1) 		226.8
(-3) 		539

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 491
1 2 3 4 5 6 range
A 353.7
(-1.8)
P 		583.3
(3)
P 		295.7
(-3)
P 		339.5
(-2.1)
P 		391.9
(-1)
P 		354.8
(-1.8)
P 		288
B 705.5
(3)
P 		556.7
(0.8)
P 		300.7
(-3)
P 		407.4
(-1.4)
P 		495.5
(-0.1)
P 		475.5
(-0.4)
P 		405
C 722.6
(3)
P 		483.9
(0.1)
P 		376.8
(-1.2)
P 		231.6
(-3)
P 		427.5
(-0.6)
P 		431.6
(-0.6)
P 		491
avr 593.9
(3) 		541.3
(1.8) 		324.4
(-3) 		326.2
(-3) 		438.3
(-0.5) 		420.6
(-0.9) 		270
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191717_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9490
Sequence Type Exemplar sequence
Representative Public ID C33H5.18 NCBI
Target Description C33H5.18 /REP_DB=WormBase Gene ID /WP=CE04161 /CHR=4 /FEA=Sanger Annotation /DEF=CDP-diacylglycerol synthase (ST.LOUIS) SW:P53439 protein_id:AAA82275.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068897(11), NM_068896(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068897 NCBI Caenorhabditis elegans C33H5.18b (C33H5.18) mRNA, complete cds. 11/11 None
NM_068896 NCBI Caenorhabditis elegans C33H5.18a (C33H5.18) mRNA, complete cds. 11/11 None
SNAP00000004491 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7813169:7815716:-1 11/11 None
GENEFINDER00000004507 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7813169:7815828:-1 11/11 None
C33H5.18b ENSEMBL cdna:known chromosome:CEL140:IV:7812753:7815716:-1 gene:C33H5.18 11/11 A 
C33H5.18a.3 ENSEMBL cdna:known chromosome:CEL140:IV:7813169:7815718:-1 gene:C33H5.18 11/11 None
C33H5.18a.2 ENSEMBL cdna:known chromosome:CEL140:IV:7813169:7815718:-1 gene:C33H5.18 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7813174-7815722(-) 100.0 100.0
Public Domain and Genome References
Ensembl C33H5.18
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84502 Hypothetical protein C33H5.18b [Caenorhabditis elegans] ref|NP_501298.1| C33H5.18b [Caenorhabditis elegans] 0.0
blast AAA82275 Hypothetical protein C33H5.18a [Caenorhabditis elegans] ref|NP_501297.1| C33H5.18a [Caenorhabditis elegans] sp|P53439|CDS1_CAEEL Putative phosphatidate cytidylyltransferase (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDP-DAG synthase) (CDP-DG synthetase) 0.0
blast CAE70893 Hypothetical protein CBG17684 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000374 EMBL-EBI Phosphatidate cytidylyltransferase 1.0E-126
Pfam IPR000374 EMBL-EBI Phosphatidate cytidylyltransferase 1.0E-126
Pfam IPR000374 EMBL-EBI Phosphatidate cytidylyltransferase 1.3E-70
Pfam IPR000374 EMBL-EBI Phosphatidate cytidylyltransferase 7.8E-46
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501298.1 8 95-117,121-143,156-178,188-210,223-245,250-272,285-307,362-384
NP_501297.1 8 85-107,111-133,146-168,178-200,213-235,240-262,275-297,352-374
C33H5.18b 8 95-117,121-143,156-178,188-210,223-245,250-272,285-307,362-384
C33H5.18a.3 8 85-107,111-133,146-168,178-200,213-235,240-262,275-297,352-374
C33H5.18a.2 8 85-107,111-133,146-168,178-200,213-235,240-262,275-297,352-374
SNAP00000004491 8 85-107,111-133,146-168,178-200,213-235,240-262,275-297,352-374
GENEFINDER00000004507 8 121-140,147-169,184-206,213-235,250-268,275-297,312-334,441-463
Sequence
Target Sequence 
>C. ELEGANS:191717_S_AT
ctggctctttacaatcgtccattcttcgtgtgtccagtacaacactatcagactgatagc
tcaaattgcacaattccattggcattccaacttcaagattatccagttccacgtccattc
tcatttgtctataaaattttacgaaaagagccaattatccaactgtgtccattcgttttt
cacagtattgcattgagtttgttcgcttcgattctgggaccattcggtggtttctttgct
agtggattcaaaagagctttcaagattaaagactttggagatgtcattcccggacatgga
ggactgatggatcgattcgattgccaacttttaatgggaacatttgtaatggtttatatt
catagtttcattcgtgttcctgatgcttcaaaacttcttaaacaaatcatgacactcgaa
ccacaagatcaactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGGCTCTTTACAATCGTCCATTCT 398 241 895 Antisense
TCCATTCTTCGTGTGTCCAGTACAA 623 589 912 Antisense
TTCAAGATTATCCAGTTCCACGTCC 616 683 986 Antisense
GAGCCAATTATCCAACTGTGTCCAT 209 385 1042 Antisense
ATTGCATTGAGTTTGTTCGCTTCGA 197 7 1081 Antisense
TTCGCTTCGATTCTGGGACCATTCG 617 667 1096 Antisense
CCATTCGGTGGTTTCTTTGCTAGTG 79 271 1114 Antisense
GACTTTGGAGATGTCATTCCCGGAC 123 373 1165 Antisense
GGATCGATTCGATTGCCAACTTTTA 185 513 1203 Antisense
TTCATTCGTGTTCCTGATGCTTCAA 607 689 1261 Antisense
GACACTCGAACCACAAGATCAACTT 28 367 1305 Antisense
Sequence Source Affymetrix Proprietary Database