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[191715_at] AMP-activated protein kinase

Description T20F7.6 /REP_DB=WormBase Gene ID /WP=CE13840 /TR=O02168 /GB=AAK18981.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=AMP-activated protein kinase
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 70      6grp_total > 05      4grp_total > 50      6grp_highest_sub > De      6grp_high1_sub > De      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 386.8
(-2.3)
P 		566.9
(3)
P 		484.1
(0.6)
P 		362.4
(-3)
P 		205
B 372.4
(-3)
P 		526
(3)
P 		471.2
(0.9)
P 		441.7
(-0.3)
P 		154
C 401.3
(-1.2)
P 		508.6
(3)
P 		502.8
(2.8)
P 		353.5
(-3)
P 		155
avr 386.8
(-3) 		533.8
(3) 		486
(1.1) 		385.9
(-3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 320.6
(3)
P 		311.8
(2.6)
P 		203.2
(-2.5)
P 		193.5
(-3)
P 		239.5
(-0.8)
P 		212.1
(-2.1)
P 		127
B 378.5
(3)
P 		320.8
(1.2)
P 		232.9
(-1.5)
P 		209.2
(-2.3)
P 		301.4
(0.6)
P 		185.2
(-3)
P 		193
C 439.5
(3)
P 		330.2
(0.4)
P 		258.3
(-1.3)
P 		209.7
(-2.5)
P 		199.8
(-2.8)
P 		189.4
(-3)
P 		250
avr 379.5
(3) 		320.9
(1.1) 		231.5
(-1.8) 		204.1
(-2.7) 		246.9
(-1.3) 		195.6
(-3) 		184
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 140.8
(3)
P 		123.1
(1.8)
P 		126.6
(2)
P 		53
(-3)
P 		88
B 118.7
(1.1)
P 		129.9
(3)
P 		109.2
(-0.5)
P 		94.1
(-3)
P 		36
C 132.8
(0.8)
P 		146.2
(1.5)
P 		175.2
(3)
P 		59.6
(-3)
P 		116
avr 130.8
(2.5) 		133.1
(2.7) 		137
(3) 		68.9
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 59.1
(-1.7)
P 		110.9
(3)
P 		44.2
(-3)
P 		49.1
(-2.6)
P 		72.3
(-0.5)
P 		51.6
(-2.3)
P 		67
B 131.7
(3)
P 		106.8
(1)
P 		56.5
(-2.9)
P 		55.4
(-3)
P 		122.5
(2.3)
P 		69.1
(-1.9)
P 		76
C 162.9
(3)
P 		100.8
(-0.1)
P 		65.8
(-1.8)
P 		42.2
(-3)
P 		64.7
(-1.9)
P 		63.4
(-1.9)
P 		121
avr 117.9
(3) 		106.2
(2) 		55.5
(-2.4) 		48.9
(-3) 		86.5
(0.3) 		61.4
(-1.9) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191715_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17482
Sequence Type Exemplar sequence
Representative Public ID T20F7.6 NCBI
Target Description T20F7.6 /REP_DB=WormBase Gene ID /WP=CE13840 /TR=O02168 /GB=AAK18981.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=AMP-activated protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078324(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078324 NCBI Caenorhabditis elegans T20F7.6 (T20F7.6) mRNA, complete cds. 9/11 None
SNAP00000037348 ENSEMBL cdna:SNAP chromosome:CEL140:X:16866310:16868887:-1 9/11 None
GENEFINDER00000037352 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16866310:16868929:-1 9/11 None
T20F7.6.1 ENSEMBL cdna:known chromosome:CEL140:X:16865628:16868944:-1 gene:T20F7.6 9/11 A 
T20F7.6.2 ENSEMBL cdna:known chromosome:CEL140:X:16865994:16869296:-1 gene:T20F7.6 9/11 A 
T20F7.6.3 ENSEMBL cdna:known chromosome:CEL140:X:16866006:16868949:-1 gene:T20F7.6 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16865850-16868929(-) 100.0 100.0
Public Domain and Genome References
Gene Title AMP-activated protein kinase
Ensembl T20F7.6
Entrez Gene 181736 Entrez gene
SwissProt O02168 EMBL-EBI
WormBase CE29350 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18981 Hypothetical protein T20F7.6 [Caenorhabditis elegans] ref|NP_510725.1| T20F7.6 [Caenorhabditis elegans] 0.0
blast T25899 hypothetical protein T20F7.6 - Caenorhabditis elegans 0.0
blast CAE69907 Hypothetical protein CBG16259 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000644 EMBL-EBI CBS domain 0.0035
Pfam IPR000644 EMBL-EBI CBS domain 1.0E-7
Pfam IPR000644 EMBL-EBI CBS domain 2.7E-5
Pfam IPR000644 EMBL-EBI CBS domain 3.5E-10
Pfam IPR000644 EMBL-EBI CBS domain 0.0035
Pfam IPR000644 EMBL-EBI CBS domain 1.0E-7
Pfam IPR000644 EMBL-EBI CBS domain 2.7E-5
Pfam IPR000644 EMBL-EBI CBS domain 3.5E-10
Sequence
Target Sequence 
>C. ELEGANS:191715_AT
gaagcgttggcgttcaaatctcaaggaggaccaatgaagaatgatgagcgtgtagtatct
gttcgggacaatgagagtttctggaaagccgtcaacgtgctggtcgaccacaatgtgcat
cgtctctgtgctgtcaatgagcacggtggtattgaaggagtgatctctctgtccgacgtt
atcaactttatggtagtgcagccggggagccatctcagaaacatcacagctccaaagaag
cattgggctcgccatcacactggagatatgaatgataagctcggtaaagttccacgaatg
ttgaaagttgtgcacacttctccgccgttctccagtttttcttggagctccgagagattc
ttctcaccaacgctgccaactttgagcacgagtcgtcaaattcaagcagtccgtccaact
cggcactgccaagcaaccagaatagctactcaaaacccccaacacatcatcactgaaatc
acttcattttacgttttcattcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCGTTGGCGTTCAAATCTCAAG 488 343 874 Antisense
TGAGCGTGTAGTATCTGTTCGGGAC 595 569 918 Antisense
TGGAAAGCCGTCAACGTGCTGGTCG 693 545 955 Antisense
ACAATGTGCATCGTCTCTGTGCTGT 596 113 983 Antisense
ATCTCTCTGTCCGACGTTATCAACT 653 31 1036 Antisense
GCCATCTCAGAAACATCACAGCTCC 383 281 1082 Antisense
AAAGAAGCATTGGGCTCGCCATCAC 584 123 1107 Antisense
TTGAAAGTTGTGCACACTTCTCCGC 441 705 1174 Antisense
TCACCAACGCTGCCAACTTTGAGCA 561 625 1237 Antisense
GTCGTCAAATTCAAGCAGTCCGTCC 592 471 1265 Antisense
TCACTTCATTTTACGTTTTCATTCC 470 627 1352 Antisense
Sequence Source Affymetrix Proprietary Database