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[191714_at] guanylate cyclase

Description F21H7.9 /REP_DB=WormBase Gene ID /WP=CE15878 /GEN=gcy-20 /TR=O62179 /GB=CAB07591.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster 6grp_total > 56      4grp_total > 23      6grp_cgc > 47      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 17.6
(2.1)
A 		3.9
(-3)
A 		3.8
(-3)
A 		20
(3)
A 		16
B 51.9
(3)
A 		6.8
(-2.5)
A 		2.6
(-3)
A 		34.3
(0.9)
A 		49
C 25.2
(3)
A 		4.5
(-2.5)
A 		2.7
(-3)
A 		11.9
(-0.5)
A 		23
avr 31.6
(3) 		5.1
(-2.6) 		3
(-3) 		22.1
(1) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 3.4
(-2)
A 		2.1
(-3)
A 		2.4
(-2.8)
A 		2.4
(-2.8)
A 		3.9
(-1.6)
A 		9.9
(3)
A 		8
B 2.7
(-2.6)
A 		9
(3)
A 		6.9
(1.1)
A 		2.2
(-3)
A 		7.7
(1.9)
A 		5
(-0.5)
A 		7
C 20.7
(-1.1)
A 		9.2
(-2.3)
A 		25
(-0.7)
A 		3.3
(-2.9)
A 		2.2
(-3)
A 		60.7
(3)
A 		59
avr 8.9
(-1.3) 		6.8
(-1.9) 		11.4
(-0.7) 		2.6
(-3) 		4.6
(-2.5) 		25.2
(3) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 6.4
(3)
A 		0.9
(-3)
A 		1
(-2.9)
A 		2.9
(-0.8)
A 		6
B 16.5
(3)
A 		1.7
(-2.6)
A 		0.6
(-3)
A 		7.3
(-0.5)
A 		16
C 8.3
(3)
A 		1.3
(-2.7)
A 		0.9
(-3)
A 		2
(-2.1)
A 		7
avr 10.4
(3) 		1.3
(-2.7) 		0.8
(-3) 		4.1
(-0.9) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 0.6
(-2.7)
A 		0.7
(-2.4)
A 		0.5
(-3)
A 		0.6
(-2.7)
A 		1.2
(-0.8)
A 		2.4
(3)
A 		2
B 0.9
(-2.3)
A 		3
(2.8)
A 		1.7
(-0.4)
A 		0.6
(-3)
A 		3.1
(3)
A 		1.9
(0.1)
A 		3
C 7.7
(-0.9)
A 		2.8
(-2.4)
A 		6.4
(-1.3)
A 		0.7
(-3)
A 		0.7
(-3)
A 		20.3
(3)
A 		20
avr 3.1
(-1) 		2.2
(-1.7) 		2.9
(-1.2) 		0.6
(-3) 		1.7
(-2.1) 		8.2
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191714_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13734
Sequence Type Exemplar sequence
Representative Public ID F21H7.9 NCBI
Target Description F21H7.9 /REP_DB=WormBase Gene ID /WP=CE15878 /GEN=gcy-20 /TR=O62179 /GB=CAB07591.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074700(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074700 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-20) (gcy-20) mRNA, complete cds. 11/11 None
SNAP00000014727 ENSEMBL cdna:SNAP chromosome:CEL140:V:16251166:16252154:-1 11/11 None
F21H7.9 ENSEMBL cdna:known chromosome:CEL140:V:16251166:16256370:-1 gene:F21H7.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16251165-16256370(-) 97.06 100.0
Public Domain and Genome References
Gene Title guanylate cyclase
Gene Symbol gcy-20
Ensembl F21H7.9
Entrez Gene 184797 Entrez gene
SwissProt O62179 EMBL-EBI
WormBase CE28919 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07591 Hypothetical protein F21H7.9 [Caenorhabditis elegans] ref|NP_507101.1| Guanylyl CYclase family member (gcy-20) [Caenorhabditis elegans] 0.0
blast CAE60828 Hypothetical protein CBG04537 [Caenorhabditis briggsae] 0.0
blast CAB01533 Hypothetical protein ZC412.2 [Caenorhabditis elegans] ref|NP_506660.1| Guanylyl CYclase family member (gcy-14) [Caenorhabditis elegans] 0.0
blast CAB07591 Hypothetical protein F21H7.9 [Caenorhabditis elegans] ref|NP_507101.1| Guanylyl CYclase family member (gcy-20) [Caenorhabditis elegans] 1.0E-141
blast CAE60828 Hypothetical protein CBG04537 [Caenorhabditis briggsae] 1.0E-117
blast CAB01533 Hypothetical protein ZC412.2 [Caenorhabditis elegans] ref|NP_506660.1| Guanylyl CYclase family member (gcy-14) [Caenorhabditis elegans] 1.0E-116
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.8E-16
Pfam IPR001054 EMBL-EBI Guanylate cyclase 8.1E-89
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 2.9E-48
Pfam IPR001054 EMBL-EBI Guanylate cyclase 8.1E-89
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507101.1 2 412-434,476-498
F21H7.9 2 412-434,476-498
Sequence
Target Sequence 
>C. ELEGANS:191714_AT
ctttgtgtttctggtttgccgcatagaaatggaaatgatcatatccgacatatagctcgg
atgtcacttggatttctgtcaagtctggaatttttccgagtgcaacatttgccggctgaa
cgcataaaccttagaattggaattaattgcggttccgtggtagctggagtcgtcgggcta
acaatgccgcgatactgtctatttggagatgcagtgaacacagctagccggatggagagt
aatggaaaacctggccaaattcacgtcactgctgaagcaaatcgaatgttgactcaagtg
gtcggtggtttcagaacggaatctcggggagaagttatcatcaaaggcaaaggagtcatg
gaaacattctggctccttggagaagaatccggatatactgccccatcaaaagctgcaccg
aaagtgattcaacatcgccagtcaattagaagcatttctccgattttggagaaaaatgca
gagggaagtgagacatcatctctctcagttgacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTTGTGTTTCTGGTTTGCCGCATA 174 223 2902 Antisense
AGCTCGGATGTCACTTGGATTTCTG 649 79 2955 Antisense
TGCAACATTTGCCGGCTGAACGCAT 684 575 3002 Antisense
TTGCGGTTCCGTGGTAGCTGGAGTC 554 697 3048 Antisense
GTCGTCGGGCTAACAATGCCGCGAT 118 473 3070 Antisense
GCAGTGAACACAGCTAGCCGGATGG 310 309 3112 Antisense
AAAACCTGGCCAAATTCACGTCACT 198 129 3147 Antisense
GTCATGGAAACATTCTGGCTCCTTG 10 467 3256 Antisense
GATATACTGCCCCATCAAAAGCTGC 432 425 3293 Antisense
AGTGATTCAACATCGCCAGTCAATT 703 59 3324 Antisense
GAGACATCATCTCTCTCAGTTGACC 475 393 3391 Antisense
Sequence Source Affymetrix Proprietary Database