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[191712_s_at]

Description C05E4.9 /REP_DB=WormBase Gene ID /WP=CE23521 /GEN=gei-7 /TR=O17353 /GB=AAB71278.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=isocitrate lyase
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 64      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 19      6grp_total > 64      4grp_total > 13      6grp_cgc > 91      6grp_cgc_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18375
1 2 3 4 range
A 22297.2
(-1.1)
P 		19066
(-2.2)
P 		16789.3
(-3)
P 		33790.1
(3)
P 		17001
B 24626.1
(0.9)
P 		18303.1
(-2.6)
P 		17593.2
(-3)
P 		28433.3
(3)
P 		10840
C 20738.9
(-0.4)
P 		18412
(-1.5)
P 		15415.4
(-3)
P 		27786.6
(3)
P 		12371
avr 22554.1
(-0.3) 		18593.7
(-2.1) 		16599.3
(-3) 		30003.3
(3) 		13404

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11038
1 2 3 4 5 6 range
A 25586.8
(1.8)
P 		19000.5
(-3)
P 		21774.7
(-1)
P 		22934.1
(-0.1)
P 		19574.3
(-2.6)
P 		27210.6
(3)
P 		8210
B 19720.7
(-0.5)
P 		16488.8
(-3)
P 		24338.4
(3)
P 		21123.6
(0.5)
P 		17046.8
(-2.6)
P 		19313.7
(-0.8)
P 		7850
C 18838.7
(-1.2)
P 		16172.4
(-3)
P 		25279.3
(3)
P 		21975.6
(0.8)
P 		21527.3
(0.5)
P 		18769.1
(-1.3)
P 		9107
avr 21382.1
(0.8) 		17220.6
(-3) 		23797.5
(3) 		22011.1
(1.4) 		19382.8
(-1) 		21764.5
(1.1) 		6577
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4042
1 2 3 4 range
A 8118.4
(3)
P 		4138.7
(-3)
P 		4389
(-2.6)
P 		4946.2
(-1.8)
P 		3980
B 7853.1
(3)
P 		4520.5
(-2.3)
P 		4076.8
(-3)
P 		6059.2
(0.1)
P 		3776
C 6865.1
(3)
P 		5293.6
(-1.3)
P 		5372.8
(-1.1)
P 		4685.3
(-3)
P 		2180
avr 7612.2
(3) 		4650.9
(-2.9) 		4612.9
(-3) 		5230.2
(-1.8) 		2999

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2783
1 2 3 4 5 6 range
A 4716.7
(-3)
P 		6757.5
(3)
P 		4742.1
(-2.9)
P 		5816.6
(0.2)
P 		5906.7
(0.5)
P 		6624.9
(2.6)
P 		2041
B 6861.8
(1.8)
P 		5488.4
(-3)
P 		5910.1
(-1.5)
P 		5594.9
(-2.6)
P 		6925.9
(2)
P 		7202
(3)
P 		1714
C 6982.7
(3)
P 		4934.7
(-1.8)
P 		6434
(1.7)
P 		4419.3
(-3)
P 		6965.9
(3)
P 		6283.1
(1.4)
P 		2563
avr 6187.1
(0.8) 		5726.9
(-1.1) 		5695.4
(-1.2) 		5276.9
(-3) 		6599.5
(2.6) 		6703.3
(3) 		1426
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191712_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15568
Sequence Type Exemplar sequence
Representative Public ID C05E4.9 NCBI
Target Description C05E4.9 /REP_DB=WormBase Gene ID /WP=CE23521 /GEN=gei-7 /TR=O17353 /GB=AAB71278.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=isocitrate lyase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070905(11), NM_001026196(10), U23159(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070905 NCBI Caenorhabditis elegans GEX Interacting protein family member (gei-7) (gei-7) mRNA, complete cds. 11/11 A 
NM_001026196 NCBI Caenorhabditis elegans GEX Interacting protein family member (gei-7) (gei-7) mRNA, complete cds. 10/11 None
U23159 NCBI Caenorhabditis elegans bifunctional glyoxylate cycle protein, complete cds. 11/11 A 
SNAP00000009755 ENSEMBL cdna:SNAP chromosome:CEL140:V:724287:727787:-1 11/11 None
GENEFINDER00000009773 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:724363:727787:-1 11/11 None
C05E4.9a.3 ENSEMBL cdna:known chromosome:CEL140:V:724037:727787:-1 gene:C05E4.9 11/11 A 
C05E4.9a.4 ENSEMBL cdna:known chromosome:CEL140:V:724040:727787:-1 gene:C05E4.9 11/11 A 
C05E4.9b.1 ENSEMBL cdna:known chromosome:CEL140:V:724040:727933:-1 gene:C05E4.9 11/11 A 
C05E4.9b.2 ENSEMBL cdna:known chromosome:CEL140:V:724040:728027:-1 gene:C05E4.9 10/11 A 
C05E4.9a.2 ENSEMBL cdna:known chromosome:CEL140:V:724101:727787:-1 gene:C05E4.9 11/11 A 
C05E4.9a.1 ENSEMBL cdna:known chromosome:CEL140:V:724154:727811:-1 gene:C05E4.9 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:724286-727787(-) 100.0 100.0
Public Domain and Genome References
Ensembl C05E4.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71278 Gex interacting protein protein 7, isoform a [Caenorhabditis elegans] ref|NP_503306.1| GEX Interacting protein family member (gei-7) [Caenorhabditis elegans] sp|Q10663|GCP_CAEEL Bifunctional glyoxylate cycle protein (GEX interacting protein 7) [Includes: Isocitrate lyase (Isocitrase) (Isocitratase) (ICL); Malate synthase ] 0.0
blast AAA85857 bifunctional glyoxylate cycle protein 0.0
blast AAN84883 Gex interacting protein protein 7, isoform b [Caenorhabditis elegans] ref|NP_001021367.1| GEX Interacting protein family member (gei-7) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 1.0E-126
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 1.0E-126
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 1.0E-126
Pfam IPR000918 EMBL-EBI Isocitrate lyase and phosphorylmutase 1.0E-126
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Pfam IPR001465 EMBL-EBI Malate synthase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191712_S_AT
gaggtatggctgcccagattccaatcaagcacgaccaggtcgccaatgacaaggcatttg
ccctagtcagagctgacaaggaacgagaggccactgacggacacgatggaacatgggttg
ctcatccaggattggtgccattggctaagagagttttcgatcaaatgatgccaaagccaa
accagatcagcaagaatctcacccgcgccaattgcaccaaggaagacctcactgtgatcc
cagagggaacccgcactgaagctggattccgccataatatctctgtaactcttggatacc
tcgactcttggctcagaggaaccggatgcgtgccactctacaacttgatggaggacgctg
ccaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGTATGGCTGCCCAGATTCCAAT 367 401 2300 Antisense
GATTCCAATCAAGCACGACCAGGTC 101 431 2316 Antisense
GCATTTGCCCTAGTCAGAGCTGACA 78 309 2353 Antisense
GGAACATGGGTTGCTCATCCAGGAT 461 527 2407 Antisense
GAAGACCTCACTGTGATCCCAGAGG 455 339 2521 Antisense
GGATTCCGCCATAATATCTCTGTAA 375 509 2563 Antisense
CTCTGTAACTCTTGGATACCTCGAC 444 229 2580 Antisense
ATACCTCGACTCTTGGCTCAGAGGA 15 23 2595 Antisense
CTCAGAGGAACCGGATGCGTGCCAC 440 229 2611 Antisense
GCGTGCCACTCTACAACTTGATGGA 174 293 2627 Antisense
AACTTGATGGAGGACGCTGCCACCG 436 137 2641 Antisense
Sequence Source Affymetrix Proprietary Database