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[191700_s_at]

Description B0545.1C /REP_DB=WormBase Gene ID /WP=CE17355 /CHR=4 /FEA=Sanger Annotation /DEF=locus:tpa-1 protein kinase (ST.LOUIS) TR:O76664 protein_id:AAC26914.1
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 16      6grp_highest_nonorm > 70      6grp_total > 16      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 309
1 2 3 4 range
A 455.8
(3)
P 		171
(-2.5)
A 		147.2
(-3)
A 		241
(-1.2)
P 		309
B 436.7
(3)
P 		253.6
(-1)
A 		158.6
(-3)
P 		292.6
(-0.1)
P 		278
C 454.4
(3)
P 		257.8
(-1.2)
A 		171.7
(-3)
P 		274.3
(-0.8)
P 		283
avr 449
(3) 		227.5
(-1.6) 		159.2
(-3) 		269.3
(-0.7) 		290

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 508
1 2 3 4 5 6 range
A 396.3
(2.6)
P 		412.4
(3)
P 		204.9
(-2.2)
P 		174.5
(-3)
P 		231.4
(-1.6)
P 		259.9
(-0.8)
P 		238
B 349.4
(3)
P 		285.4
(1)
P 		250.2
(-0.2)
P 		161.4
(-3)
P 		172.9
(-2.6)
P 		192.2
(-2)
P 		188
C 669.3
(3)
P 		364.2
(-1.1)
P 		318.6
(-1.7)
P 		250.8
(-2.6)
P 		249.8
(-2.6)
P 		218.2
(-3)
P 		451
avr 471.7
(3) 		354
(0.4) 		257.9
(-1.6) 		195.6
(-3) 		218
(-2.5) 		223.4
(-2.4) 		276
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 165.9
(3)
P 		37.1
(-2.9)
A 		38.5
(-2.9)
A 		35.3
(-3)
P 		131
B 139.3
(3)
P 		62.6
(-1.5)
A 		36.7
(-3)
P 		62.3
(-1.5)
P 		103
C 150.4
(3)
P 		74.1
(-1.4)
A 		59.8
(-2.2)
P 		46.3
(-3)
P 		104
avr 151.9
(3) 		57.9
(-2.3) 		45
(-3) 		48
(-2.8) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 73.1
(-1.3)
P 		146.7
(3)
P 		44.6
(-3)
P 		44.3
(-3)
P 		69.8
(-1.5)
P 		63.3
(-1.9)
P 		102
B 121.6
(3)
P 		95
(1)
P 		60.7
(-1.6)
P 		42.7
(-3)
P 		70.2
(-0.9)
P 		71.7
(-0.8)
P 		79
C 248.1
(3)
P 		111.1
(-1.2)
P 		81.1
(-2.1)
P 		50.4
(-3)
P 		80.8
(-2.1)
P 		73
(-2.3)
P 		198
avr 147.6
(3) 		117.6
(1.2) 		62.1
(-2) 		45.8
(-3) 		73.6
(-1.4) 		69.3
(-1.6) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191700_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10991
Sequence Type Exemplar sequence
Representative Public ID B0545.1C NCBI
Target Description B0545.1C /REP_DB=WormBase Gene ID /WP=CE17355 /CHR=4 /FEA=Sanger Annotation /DEF=locus:tpa-1 protein kinase (ST.LOUIS) TR:O76664 protein_id:AAC26914.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067459(11), NM_067460(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067459 NCBI Caenorhabditis elegans TPA (tetradecanoyl phorbol acetate) resistant family member (tpa-1) (tpa-1) mRNA, complete cds. 11/11 None
NM_067460 NCBI Caenorhabditis elegans TPA (tetradecanoyl phorbol acetate) resistant family member (tpa-1) (tpa-1) mRNA, complete cds. 11/11 None
B0545.1a ENSEMBL cdna:known chromosome:CEL140:IV:92016:110604:1 gene:B0545.1 11/11 None
B0545.1b.2 ENSEMBL cdna:known chromosome:CEL140:IV:92803:110707:1 gene:B0545.1 11/11 None
B0545.1b.1 ENSEMBL cdna:known chromosome:CEL140:IV:103444:110707:1 gene:B0545.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028006 4/11 Cross Hyb Matching Probes None
GENEFINDER00000044547 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:103519-110151(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0545.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC26916 Tpa (tetradecanoyl phorbol acetate) resistant protein 1, isoform a [Caenorhabditis elegans] ref|NP_499860.1| TPA (tetradecanoyl phorbol acetate) resistant family member (tpa-1) [Caenorhabditis elegans] dbj|BAA08470.1| TPA-1A [Caenorhabditis elegans] sp|P34722|KPC1_CAEEL Protein kinase C-like 1 (PKC) (Tetradecanoyl phorbol acetate resistant protein 1) 0.0
blast CAE58497 Hypothetical protein CBG01645 [Caenorhabditis briggsae] 0.0
blast T33399 protein kinase C homolog tpa-1, splice form 1 - Caenorhabditis elegans 0.0
blast AAC26917 Tpa (tetradecanoyl phorbol acetate) resistant protein 1, isoform b [Caenorhabditis elegans] ref|NP_499861.1| TPA (tetradecanoyl phorbol acetate) resistant family member (tpa-1) [Caenorhabditis elegans] dbj|BAA08471.1| TPA-1B [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-51
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-51
hanks 1.2.11 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; CeTPA1 1.0E-153
hanks 1.2.11 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; CeTPA1 1.0E-155
Protein Domains
Database ID Description E-Value
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 6.8E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.8E-24
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-71
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-32
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 6.8E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.8E-24
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-71
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-32
Sequence
Target Sequence 
>C. ELEGANS:191700_S_AT
gatggtcacataaagcttgccgattttggtatggcaaaaaccgagatgaatcgagaaaac
ggaatggctagcacattctgtggaacaccggattatatcagtcctgaaattatcaagggt
caactttacaatgaagctgtcgatttttggtcatttggagttttgatgtatgaaatgctc
gtcggacaatcaccgttccatggagaaggagaagatgaattatttgattcaattcttaat
gagaggccatatttcccgaaaactatatcgaaggaagcagcgaaatgtctatcagcctta
ttcgatagaaatccgaatacccggctaggaatgcctgaatgccctgatggtccaatcagg
caacattgcttcttccgaggtgtcgactggaaacggtttgaaaatcgacaggttccgcca
ccgtttaagccgaacattaaatccaactcggacgcctcgaacttcgacgacgacttcacg
aacgagaaggcggcgttgacacctgtgcacgacaagaacctactggcttcaatcgatcct
gaagcattccttaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGGTCACATAAAGCTTGCCGATT 575 407 1219 Antisense
AATGCTCGTCGGACAATCACCGTTC 249 167 1392 Antisense
TGAGAGGCCATATTTCCCGAAAACT 584 567 1458 Antisense
AGCAGCGAAATGTCTATCAGCCTTA 527 77 1494 Antisense
AGGAATGCCTGAATGCCCTGATGGT 683 51 1545 Antisense
ATCAGGCAACATTGCTTCTTCCGAG 707 27 1573 Antisense
ACATTAAATCCAACTCGGACGCCTC 579 101 1652 Antisense
GAACTTCGACGACGACTTCACGAAC 565 349 1677 Antisense
ACACCTGTGCACGACAAGAACCTAC 583 109 1717 Antisense
AAGAACCTACTGGCTTCAATCGATC 549 151 1732 Antisense
ATCGATCCTGAAGCATTCCTTAACT 181 37 1750 Antisense
Sequence Source Affymetrix Proprietary Database