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[191697_at] 7TM chemoreceptor, srh family

Description F31F4.18 /REP_DB=WormBase Gene ID /WP=CE24931 /GEN=srh-246 /TR=Q9GUC2 /GB=AAG24096.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 64      4grp_total > 19      6grp_cgc > 55      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 37.8
(-2.5)
A 		68.8
(-0.2)
A 		30.7
(-3)
A 		111.6
(3)
P 		81
B 52.1
(1.1)
A 		34.4
(-1.2)
A 		20.7
(-3)
A 		67.1
(3)
M 		46
C 47.1
(-1.9)
A 		66.3
(-0.7)
A 		29.9
(-3)
A 		122.9
(3)
P 		93
avr 45.7
(-1.5) 		56.5
(-0.6) 		27.1
(-3) 		100.5
(3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 17.5
(-3)
A 		42.1
(0.3)
A 		56.8
(2.3)
A 		36.7
(-0.4)
A 		24.7
(-2)
A 		61.9
(3)
A 		44
B 12.1
(-3)
A 		46.9
(1.7)
A 		46.6
(1.7)
A 		24
(-1.4)
A 		36.7
(0.3)
A 		56.4
(3)
A 		44
C 5.7
(-3)
A 		60.7
(3)
A 		55.1
(2.4)
A 		52.4
(2.1)
M 		19.4
(-1.5)
A 		24.4
(-1)
A 		55
avr 11.8
(-3) 		49.9
(2.6) 		52.8
(3) 		37.7
(0.8) 		26.9
(-0.8) 		47.6
(2.2) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 13.7
(1.1)
A 		14.9
(2)
A 		8
(-3)
A 		16.3
(3)
P 		8
B 16.6
(3)
A 		8.5
(-1.1)
A 		4.8
(-3)
A 		14.3
(1.8)
M 		12
C 15.6
(0)
A 		19.1
(2.1)
A 		10.4
(-3)
A 		20.7
(3)
P 		10
avr 15.3
(1.9) 		14.2
(1.1) 		7.7
(-3) 		17.1
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 3.2
(-3)
A 		15
(2.9)
A 		12.4
(1.6)
A 		9.3
(0.1)
A 		7.4
(-0.9)
A 		15.1
(3)
A 		12
B 4.2
(-3)
A 		15.6
(1.1)
A 		11.3
(-0.5)
A 		6.4
(-2.2)
A 		14.9
(0.8)
A 		21
(3)
A 		17
C 2.1
(-3)
A 		18.5
(3)
A 		14
(1.4)
A 		10.5
(0.1)
M 		6.3
(-1.5)
A 		8.2
(-0.8)
A 		16
avr 3.2
(-3) 		16.4
(3) 		12.6
(1.3) 		8.7
(-0.5) 		9.5
(-0.1) 		14.8
(2.3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191697_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15683
Sequence Type Exemplar sequence
Representative Public ID F31F4.18 NCBI
Target Description F31F4.18 /REP_DB=WormBase Gene ID /WP=CE24931 /GEN=srh-246 /TR=Q9GUC2 /GB=AAG24096.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070881(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070881 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-246) (srh-246) mRNA, complete cds. 11/11 None
SNAP00000012363 ENSEMBL cdna:SNAP chromosome:CEL140:V:656329:657470:-1 11/11 None
GENEFINDER00000012374 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:656329:657470:-1 11/11 None
F31F4.18 ENSEMBL cdna:known chromosome:CEL140:V:656329:657608:-1 gene:F31F4.18 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:656328-657608(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-246
Ensembl F31F4.18
Entrez Gene 191893 Entrez gene
SwissProt Q9GUC2 EMBL-EBI
WormBase CE24931 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503282 Serpentine Receptor, class H family member (srh-246) [Caenorhabditis elegans] gb|AAG24096.1| Serpentine receptor, class h protein 246 [Caenorhabditis elegans] 1.0E-169
blast T32306 hypothetical protein F31F4.9 - Caenorhabditis elegans 1.0E-158
blast NP_503282 Serpentine Receptor, class H family member (srh-246) [Caenorhabditis elegans] gb|AAG24096.1| Serpentine receptor, class h protein 246 [Caenorhabditis elegans] 1.0E-140
blast NP_503281 Serpentine Receptor, class H family member (srh-245) [Caenorhabditis elegans] gb|AAG24097.1| Serpentine receptor, class h protein 245 [Caenorhabditis elegans] 1.0E-134
blast T32306 hypothetical protein F31F4.9 - Caenorhabditis elegans 1.0E-129
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.3E-97
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503282.1 7 20-42,55-77,92-114,135-154,189-211,240-262,272-294
F31F4.18 7 20-42,55-77,92-114,135-154,189-211,240-262,272-294
SNAP00000012363 6 20-42,46-68,89-108,143-165,194-216,226-248
GENEFINDER00000012374 6 20-42,46-68,89-108,143-165,194-216,226-248
Sequence
Target Sequence 
>C. ELEGANS:191697_AT
aatatgcttatttgttcccacgttcctgatgatcccttttatattcctgccggagcaaga
agccgctagagctctagtttttgaaaggcttccatgcctcagatatatcaatacttatga
cagagaaatgtttgtatttgcgattgatccaattatcccagcggtgtgcactactgtggt
ggtaaccgtcatgattacgccaacagtcacatttttcgttctgacgctgaaaaatctgtt
caaggagaacaaggttttcaatatatctcggaaaacatttgagacccagcagactttctt
caaggcgataacgattcagttcatgttcttcttgttactggtgatcattcccattatagt
gattttgatcatcgatttcacagcttatcacaaccaattgctcaataatatcatactttt
tccattctatctctcaggagtttggtccacacttatcatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATATGCTTATTTGTTCCCACGTTC 518 173 426 Antisense
CTGATGATCCCTTTTATATTCCTGC 353 239 451 Antisense
GAAAGGCTTCCATGCCTCAGATATA 378 361 508 Antisense
ATCCAATTATCCCAGCGGTGTGCAC 336 41 572 Antisense
GGTGTGCACTACTGTGGTGGTAACC 305 499 588 Antisense
CCGTCATGATTACGCCAACAGTCAC 247 265 611 Antisense
GTCACATTTTTCGTTCTGACGCTGA 41 465 631 Antisense
TTTGAGACCCAGCAGACTTTCTTCA 176 665 703 Antisense
GATTCAGTTCATGTTCTTCTTGTTA 409 431 738 Antisense
ATCATACTTTTTCCATTCTATCTCT 75 31 835 Antisense
GGAGTTTGGTCCACACTTATCATGC 535 517 862 Antisense
Sequence Source Affymetrix Proprietary Database