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[191692_at] guanylate cyclase

Description ZK455.2 /REP_DB=WormBase Gene ID /WP=CE03813 /GEN=gcy-9 /TR=Q23496 /GB=CAA91488.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster 6grp_total > 18      4grp_total > 30      6grp_cgc > 01      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 127
(3)
A 		96.5
(-3)
A 		113.8
(0.4)
A 		110.2
(-0.3)
A 		31
B 103.1
(3)
A 		79.7
(-0.7)
A 		65.6
(-3)
A 		83.1
(-0.2)
A 		38
C 103.5
(-2.2)
A 		129.9
(3)
A 		99.6
(-3)
A 		121.8
(1.4)
A 		30
avr 111.2
(3) 		102
(-0) 		93
(-3) 		105
(1) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 215.1
(3)
A 		94.1
(-1.5)
A 		52.4
(-3)
A 		116.9
(-0.6)
A 		105.4
(-1)
A 		90.2
(-1.6)
A 		163
B 165.9
(3)
A 		107.1
(-2.1)
A 		96.5
(-3)
A 		107.1
(-2.1)
A 		121.8
(-0.8)
A 		98.1
(-2.9)
A 		69
C 162.5
(3)
A 		95.9
(-1.6)
A 		91.5
(-1.9)
A 		74.9
(-3)
A 		107.9
(-0.7)
A 		126.1
(0.5)
A 		88
avr 181.2
(3) 		99
(-1.9) 		80.1
(-3) 		99.6
(-1.8) 		111.7
(-1.1) 		104.8
(-1.5) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 46.2
(3)
A 		21
(-2)
A 		29.8
(-0.3)
A 		16.1
(-3)
A 		30
B 32.9
(3)
A 		19.7
(-1.5)
A 		15.2
(-3)
A 		17.7
(-2.2)
A 		18
C 34.3
(1.9)
A 		37.3
(3)
A 		34.7
(2.1)
A 		20.5
(-3)
A 		17
avr 37.8
(3) 		26
(-0.6) 		26.6
(-0.4) 		18.1
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 39.6
(3)
A 		33.5
(1.7)
A 		11.4
(-3)
A 		29.6
(0.9)
A 		31.8
(1.3)
A 		22
(-0.7)
A 		28
B 57.7
(3)
A 		35.7
(-0.8)
A 		23.4
(-3)
A 		28.4
(-2.1)
A 		49.5
(1.6)
A 		36.6
(-0.7)
A 		34
C 60.2
(3)
A 		29.3
(-1.1)
A 		23.3
(-1.9)
A 		15.1
(-3)
A 		34.9
(-0.4)
A 		42.2
(0.6)
A 		45
avr 52.5
(3) 		32.8
(-0.6) 		19.4
(-3) 		24.4
(-2.1) 		38.7
(0.5) 		33.6
(-0.4) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191692_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17111
Sequence Type Exemplar sequence
Representative Public ID ZK455.2 NCBI
Target Description ZK455.2 /REP_DB=WormBase Gene ID /WP=CE03813 /GEN=gcy-9 /TR=Q23496 /GB=CAA91488.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077496(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077496 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-9) (gcy-9) mRNA, complete cds. 11/11 None
SNAP00000004976 ENSEMBL cdna:SNAP chromosome:CEL140:X:11332477:11337444:-1 10/11 None
GENEFINDER00000004983 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11332477:11337793:-1 11/11 None
ZK455.2 ENSEMBL cdna:known chromosome:CEL140:X:11332512:11337995:-1 gene:ZK455.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11332511-11337995(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanylate cyclase
Gene Symbol gcy-9
Ensembl ZK455.2
Entrez Gene 191645 Entrez gene
SwissProt Q23496 EMBL-EBI
WormBase CE03813 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91488 Hypothetical protein ZK455.2 [Caenorhabditis elegans] ref|NP_509897.1| Guanylyl CYclase family member (gcy-9) [Caenorhabditis elegans] 0.0
blast CAE70655 Hypothetical protein CBG17362 [Caenorhabditis briggsae] 0.0
blast CAB05325 Hypothetical protein ZK896.8 [Caenorhabditis elegans] ref|NP_502449.1| Guanylyl CYclase family member (gcy-18) [Caenorhabditis elegans] 1.0E-163
blast CAB05325 Hypothetical protein ZK896.8 [Caenorhabditis elegans] ref|NP_502449.1| Guanylyl CYclase family member (gcy-18) [Caenorhabditis elegans] 1.0E-162
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 5.3E-70
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 7.4E-25
Pfam IPR001054 EMBL-EBI Guanylate cyclase 6.0E-84
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 7.4E-25
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.5E-77
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 6.4E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509897.1 2 348-370,491-513
ZK455.2 2 348-370,491-513
SNAP00000004976 1 383-405
GENEFINDER00000004983 2 324-346,467-489
Sequence
Target Sequence 
>C. ELEGANS:191692_AT
agccagtgcttccgcaactatatagttgtgcgactgttttgttttcggatattcgtggat
tcaccagaatttcttcaacatctacaccacttcaggtttgcaagctcagacaaaagatta
tagttgtaacgtttctaaatgacatgttcagtggattcgatgctattattgcaaaacacg
atgcatacaaagtcgaaacaattggtgacgcttacatgattgtatcaggagttcccacag
agaatggaaatagccacgctcaaaatattgctgatgtcgctttgaaaatgagagcgttta
tctgtaacttcaaacttgctcaccgccctgaagagctcatgatggtgaggattggctttc
atagtggtccagttgccgctggagttgttggtcttgccgctccacgttactgccttttcg
gggacactgttaacactgcatctagaatggaaagcaccggagtggctaataaaattcaga
tatccgagggagcatacaaccttcttcattgcttctttcctcaattccaaatggtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCCAGTGCTTCCGCAACTATATAG 185 85 2744 Antisense
GTTGTGCGACTGTTTTGTTTTCGGA 379 437 2768 Antisense
GTTTTCGGATATTCGTGGATTCACC 281 449 2784 Antisense
ACATCTACACCACTTCAGGTTTGCA 567 105 2821 Antisense
CTGTAACTTCAAACTTGCTCACCGC 243 239 3045 Antisense
CCGCCCTGAAGAGCTCATGATGGTG 559 261 3066 Antisense
GCTTTCATAGTGGTCCAGTTGCCGC 32 305 3098 Antisense
TACTGCCTTTTCGGGGACACTGTTA 502 641 3151 Antisense
GGGACACTGTTAACACTGCATCTAG 184 495 3164 Antisense
GGGAGCATACAACCTTCTTCATTGC 457 495 3231 Antisense
CTTCTTTCCTCAATTCCAAATGGTT 296 221 3255 Antisense
Sequence Source Affymetrix Proprietary Database