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[191675_s_at]

Description ZC8.4 /REP_DB=WormBase Gene ID /WP=CE07580 /CHR=X /FEA=Sanger Annotation /DEF=probable myofibrillar protein (ST.LOUIS) TR:Q23081 protein_id:AAB52624.1
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 59      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 68      6grp_total > 49      4grp_total > 46      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2487
1 2 3 4 range
A 3085.7
(3)
P 		1277.1
(-1.5)
P 		832.8
(-2.6)
P 		668.3
(-3)
P 		2417
B 3155.4
(3)
P 		1074.9
(-2.2)
P 		895.8
(-2.7)
P 		770.7
(-3)
P 		2385
C 2392.6
(3)
P 		941
(-2.2)
P 		1017
(-1.9)
P 		701.9
(-3)
P 		1691
avr 2877.9
(3) 		1097.7
(-1.9) 		915.2
(-2.4) 		713.6
(-3) 		2164

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3003
1 2 3 4 5 6 range
A 2497.4
(0.5)
P 		2745.9
(1)
P 		821.9
(-3)
P 		1660.8
(-1.3)
P 		2241.4
(-0.1)
P 		3717
(3)
P 		2895
B 2281.8
(2.1)
P 		1654.3
(0.1)
P 		714.4
(-3)
P 		1563.9
(-0.2)
P 		1477.8
(-0.5)
P 		2542
(3)
P 		1828
C 2975.9
(3)
P 		1724
(-0.7)
P 		1652.7
(-0.9)
P 		948.8
(-3)
P 		2150.2
(0.6)
P 		2356.8
(1.2)
P 		2027
avr 2585
(2) 		2041.4
(0.2) 		1063
(-3) 		1391.2
(-1.9) 		1956.5
(-0) 		2871.9
(3) 		1809
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1026
1 2 3 4 range
A 1123.5
(3)
P 		277.2
(-2)
P 		217.7
(-2.3)
P 		97.8
(-3)
P 		1026
B 1006.2
(3)
P 		265.5
(-2.3)
P 		207.6
(-2.7)
P 		164.2
(-3)
P 		842
C 792
(3)
P 		270.5
(-1.6)
P 		354.5
(-0.9)
P 		118.4
(-3)
P 		674
avr 973.9
(3) 		271.1
(-2) 		259.9
(-2.1) 		126.8
(-3) 		847

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 930
1 2 3 4 5 6 range
A 460.4
(-0.9)
P 		976.6
(3)
P 		179
(-3)
P 		421.2
(-1.2)
P 		676.4
(0.7)
P 		905
(2.5)
P 		798
B 794
(1.8)
P 		550.6
(-0.1)
P 		173.5
(-3)
P 		414.2
(-1.1)
P 		600.4
(0.3)
P 		947.9
(3)
P 		774
C 1103
(3)
P 		526.1
(-0.8)
P 		420.6
(-1.5)
P 		190.8
(-3)
P 		695.8
(0.3)
P 		789
(0.9)
P 		912
avr 785.8
(2.1) 		684.4
(1.1) 		257.7
(-3) 		342.1
(-2.2) 		657.5
(0.9) 		880.6
(3) 		623
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191675_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16795
Sequence Type Exemplar sequence
Representative Public ID ZC8.4 NCBI
Target Description ZC8.4 /REP_DB=WormBase Gene ID /WP=CE07580 /CHR=X /FEA=Sanger Annotation /DEF=probable myofibrillar protein (ST.LOUIS) TR:Q23081 protein_id:AAB52624.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076447(11), NM_001029810(11), NM_076446(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076447 NCBI Caenorhabditis elegans Lin-5 (Five) Interacting protein family member (lfi-1) (lfi-1) mRNA, complete cds. 11/11 None
NM_001029810 NCBI Caenorhabditis elegans Lin-5 (Five) Interacting protein family member (lfi-1) (lfi-1) mRNA, complete cds. 11/11 None
NM_076446 NCBI Caenorhabditis elegans Lin-5 (Five) Interacting protein family member (lfi-1) (lfi-1) mRNA, complete cds. 11/11 None
GENEFINDER00000002226 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4984045:4992822:1 11/11 None
SNAP00000002218 ENSEMBL cdna:SNAP chromosome:CEL140:X:4984490:4992822:1 10/11 None
ZC8.4a ENSEMBL cdna:known chromosome:CEL140:X:4983551:4993192:1 gene:ZC8.4 11/11 None
ZC8.4b ENSEMBL cdna:known chromosome:CEL140:X:4984045:4992822:1 gene:ZC8.4 11/11 None
ZC8.4d ENSEMBL cdna:known chromosome:CEL140:X:4984045:4992822:1 gene:ZC8.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029809 8/11 Cross Hyb Matching Probes None
ZC8.4c 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180771 4 3 NM_001029810 191675_s_at (11)
NM_076446 191675_s_at (11)
NM_076447 173059_at (11), 191675_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4984045-4992823(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZC8.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM69078 Lin-5 (five) interacting protein protein 1, isoform a [Caenorhabditis elegans] ref|NP_508848.2| Lin-5 (Five) Interacting protein family member (lfi-1) [Caenorhabditis elegans] 0.0
blast NP_508847 Lin-5 (Five) Interacting protein family member (lfi-1) [Caenorhabditis elegans] 0.0
blast AAQ91890 Lin-5 (five) interacting protein protein 1, isoform d [Caenorhabditis elegans] ref|NP_001024981.1| Lin-5 (Five) Interacting protein family member (lfi-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 0.109999999403954
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 0.100000001490116
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 0.0890000015497208
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 0.0869999974966049
Pfam IPR004827 EMBL-EBI Basic-leucine zipper (bZIP) transcription factor 2.9E-6
Pfam IPR004827 EMBL-EBI Basic-leucine zipper (bZIP) transcription factor 2.9E-6
Pfam IPR004827 EMBL-EBI Basic-leucine zipper (bZIP) transcription factor 2.9E-6
Pfam IPR004827 EMBL-EBI Basic-leucine zipper (bZIP) transcription factor 2.9E-6
Pfam IPR004827 EMBL-EBI Basic-leucine zipper (bZIP) transcription factor 2.9E-6
Sequence
Target Sequence 
>C. ELEGANS:191675_S_AT
gagcgtacccatcgtaatcgcatcaagcagcttgaagatcaaatcgcaattctccgtgat
caacttgatggagagcgtcgccgcagacgggagtatgttgatcgttccatggttaatgat
attggacgtctcggctcaaatgttcttggaattcgtaacagctatggagataacagcatt
gatgccattatcaatggtggaagccgttcagtaggattttatccacgttcgacgtttgct
tcaaatccattgactcctccactcggaagttctacaccaactcatcgtccacacgtgacc
gatttcagaagcgccgttgacgctggcagcagctatcgccgtccaatctcgacgattgaa
gattcgggatcggtgtacggaggctcgattagagatcgcgactcggtctacggtggcggt
gggcgtgattcgtcgttcggcactcgtgccggcgattccatctctcgtgcgggtgtcgag
ccacgagatccatcgctcgtcgaaattccatccaacgagccgccaatgacaacgtccacc
cattctcaaggaggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGTACCCATCGTAATCGCATCA 33 385 6283 Antisense
AAATCGCAATTCTCCGTGATCAACT 96 119 6323 Antisense
CAACTTGATGGAGAGCGTCGCCGCA 581 187 6343 Antisense
GATATTGGACGTCTCGGCTCAAATG 645 425 6400 Antisense
TATCCACGTTCGACGTTTGCTTCAA 366 657 6502 Antisense
CGGAAGTTCTACACCAACTCATCGT 90 241 6546 Antisense
TCGTCCACACGTGACCGATTTCAGA 211 605 6567 Antisense
TCAGAAGCGCCGTTGACGCTGGCAG 637 623 6587 Antisense
TTCGGGATCGGTGTACGGAGGCTCG 269 669 6645 Antisense
ATCCATCGCTCGTCGAAATTCCATC 3 41 6770 Antisense
ACGTCCACCCATTCTCAAGGAGGAT 277 93 6814 Antisense
Sequence Source Affymetrix Proprietary Database