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[191672_s_at]

Description Y73B6A.5 /REP_DB=WormBase Gene ID /WP=CE25585 /GEN=lin-45 /TR=Q9N4E3 /GB=AAF36042.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 47      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 82      6grp_total > 48      4grp_total > 04      6grp_highest_sub > Cu      6grp_cgc > 17      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 390
1 2 3 4 range
A 410.4
(0.1)
P 		312.2
(-2)
P 		263.3
(-3)
P 		548.1
(3)
P 		285
B 351.4
(-2.4)
P 		332.4
(-2.8)
P 		320.7
(-3)
P 		653.1
(3)
P 		332
C 410.6
(0.1)
P 		324.3
(-1.9)
P 		276.9
(-3)
P 		538.6
(3)
P 		262
avr 390.8
(-0.9) 		323
(-2.3) 		287
(-3) 		579.9
(3) 		293

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 507
(3)
P 		332.3
(-1.1)
P 		279.6
(-2.3)
P 		251.7
(-3)
P 		314.1
(-1.5)
P 		495.8
(2.7)
P 		255
B 339.9
(0.1)
P 		374.7
(1.3)
P 		259.2
(-2.5)
P 		289.8
(-1.5)
P 		242.3
(-3)
P 		429.1
(3)
P 		187
C 467.3
(3)
P 		350.4
(-0.8)
P 		294.9
(-2.6)
P 		287.9
(-2.8)
P 		281.1
(-3)
P 		400.2
(0.8)
P 		186
avr 438.1
(2.9) 		352.5
(-0.2) 		277.9
(-2.9) 		276.5
(-3) 		279.2
(-2.9) 		441.7
(3) 		165
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 149.4
(3)
P 		67.8
(-3)
P 		68.8
(-2.9)
P 		80.2
(-2.1)
P 		82
B 112.1
(0.5)
P 		82.1
(-2.3)
P 		74.3
(-3)
P 		139.2
(3)
P 		65
C 135.9
(3)
P 		93.2
(-2.7)
P 		96.5
(-2.2)
P 		90.8
(-3)
P 		45
avr 132.5
(3) 		81
(-2.9) 		79.9
(-3) 		103.4
(-0.3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 93.5
(0.3)
P 		118.2
(2.7)
P 		60.9
(-3)
P 		63.8
(-2.7)
P 		94.8
(0.4)
P 		120.7
(3)
P 		60
B 118.3
(0.4)
P 		124.7
(0.8)
P 		63
(-3)
P 		76.8
(-2.1)
P 		98.5
(-0.8)
P 		160
(3)
P 		97
C 173.2
(3)
P 		106.9
(-0.5)
P 		75.1
(-2.1)
P 		57.9
(-3)
P 		91
(-1.3)
P 		134
(1)
P 		115
avr 128.3
(2.2) 		116.6
(1.2) 		66.3
(-3) 		66.2
(-3) 		94.8
(-0.6) 		138.2
(3) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191672_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9471
Sequence Type Exemplar sequence
Representative Public ID Y73B6A.5 NCBI
Target Description Y73B6A.5 /REP_DB=WormBase Gene ID /WP=CE25585 /GEN=lin-45 /TR=Q9N4E3 /GB=AAF36042.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171367(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171367 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-45) (lin-45) mRNA, complete cds. 11/11 A 
SNAP00000010734 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6743222:6747388:-1 11/11 None
GENEFINDER00000010740 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6743222:6747109:-1 11/11 None
Y73B6A.5a ENSEMBL cdna:known chromosome:CEL140:IV:6742522:6747450:-1 gene:Y73B6A.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177436 2 1 NM_171367 173413_at (11), 191672_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6743227-6747394(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y73B6A.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF36042 Abnormal cell lineage protein 45, isoform a [Caenorhabditis elegans] ref|NP_741430.3| abnormal cell LINeage family member (lin-45) [Caenorhabditis elegans] gb|AAR86712.1| LIN-45 isoform 2 [Caenorhabditis elegans] gb|AAR26307.1| LIN-45 [Caenorhabditis elegans] sp|Q07292|KRAF1_CAEEL Raf homolog serine/threonine-protein kinase (Abnormal cell lineage protein 45) 0.0
blast AAA28142 raf proto-oncogene prf||1913436A lraf protein 0.0
blast AAR86713 LIN-45 isoform 3 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.2E-18
Pfam IPR000719 EMBL-EBI Protein kinase 3.2E-68
Pfam IPR003116 EMBL-EBI Raf-like Ras-binding 1.3E-35
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-63
Pfam IPR000719 EMBL-EBI Protein kinase 3.2E-68
Pfam IPR003116 EMBL-EBI Raf-like Ras-binding 1.3E-35
Sequence
Target Sequence 
>C. ELEGANS:191672_S_AT
tggagatttcggcttggcaactgtcaaaacgaaatggacagtgaacggaggtcaacagca
acaacaaccaacaggaagtattctctggatggcacctgaagtcatcagaatgcaagatga
taatccatataccccacagtctgatgtgtattcttttggaatatgcatgtacgaaattct
atcttcgcatctaccatactccaacattaataatcgagatcagatattgtttatggttgg
aagagggtatttgaggccggatagatcgaaaattcgacatgatacgccgaagagcatgct
caaattatatgacaattgcattatgttcgatagaaatgagcggccagtatttggagaggt
tctcgaacgacttcgcgacataattcttccaaaattgactcgttctcaatcagctccgaa
tgttcttcacttagactcgcaatattcggtgatggatgctgtaatgagaagccaaatgct
ttcctggtcatatattccacctgcaactgcaaaaaccccacaatcagcagcagcagcggc
cgctgctaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGATTTCGGCTTGGCAACTGTC 463 549 1869 Antisense
ATGATAATCCATATACCCCACAGTC 560 45 1985 Antisense
GCATCTACCATACTCCAACATTAAT 167 309 2055 Antisense
GGAGAGGTTCTCGAACGACTTCGCG 256 517 2221 Antisense
GACTTCGCGACATAATTCTTCCAAA 254 371 2237 Antisense
AATTGACTCGTTCTCAATCAGCTCC 166 179 2261 Antisense
ATCAGCTCCGAATGTTCTTCACTTA 103 29 2277 Antisense
AAGCCAAATGCTTTCCTGGTCATAT 380 149 2337 Antisense
TCATATATTCCACCTGCAACTGCAA 3 619 2356 Antisense
TGCAACTGCAAAAACCCCACAATCA 411 577 2370 Antisense
GCAGCAGCAGCGGCCGCTGCTAACA 508 313 2395 Antisense
Sequence Source Affymetrix Proprietary Database