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[191668_at] 7TM chemoreceptor, srh family

Description H05B21.2 /REP_DB=WormBase Gene ID /WP=CE17952 /GEN=srh-248 /TR=O61969 /GB=AAC17763.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 65      4grp_total > 03      6grp_cgc > 56      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 20
(-2.4)
A 		6.3
(-3)
A 		13.7
(-2.7)
A 		152.3
(3)
P 		146
B 26.6
(-1)
A 		5.7
(-2.7)
A 		2.5
(-3)
A 		73.5
(3)
P 		71
C 20.4
(-2.3)
A 		8
(-3)
A 		18.6
(-2.4)
A 		120.3
(3)
P 		112
avr 22.3
(-2.1) 		6.7
(-3) 		11.6
(-2.7) 		115.4
(3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 13.7
(-1.4)
A 		3.8
(-3)
A 		40.7
(3)
A 		3.9
(-3)
A 		9.6
(-2.1)
A 		28
(0.9)
A 		37
B 6.1
(-2.2)
A 		2.2
(-3)
A 		12.9
(-0.8)
A 		11.3
(-1.1)
A 		31.5
(3)
A 		30.6
(2.8)
A 		29
C 3.7
(-2.3)
A 		6.6
(0.7)
A 		6.1
(0.2)
A 		3.7
(-2.3)
A 		3
(-3)
A 		8.8
(3)
A 		6
avr 7.8
(-1.8) 		4.2
(-3) 		19.9
(2.1) 		6.3
(-2.3) 		14.7
(0.4) 		22.5
(3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 7.3
(-1.3)
A 		1.4
(-3)
A 		3.6
(-2.4)
A 		22.3
(3)
P 		21
B 8.5
(0.1)
A 		1.4
(-2.7)
A 		0.6
(-3)
A 		15.7
(3)
P 		15
C 6.8
(-1.5)
A 		2.3
(-3)
A 		6.5
(-1.6)
A 		20.3
(3)
P 		18
avr 7.5
(-1) 		1.7
(-3) 		3.6
(-2.4) 		19.4
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 2.5
(-1.9)
A 		1.3
(-2.8)
A 		8.9
(3)
A 		1
(-3)
A 		2.9
(-1.6)
A 		6.8
(1.4)
A 		8
B 2.1
(-2.3)
A 		0.7
(-3)
A 		3.1
(-1.8)
A 		3
(-1.9)
A 		12.8
(3)
A 		11.4
(2.3)
A 		12
C 1.4
(-1.2)
A 		2
(0.4)
A 		1.5
(-0.9)
A 		0.7
(-3)
A 		1
(-2.2)
A 		3
(3)
A 		2
avr 2
(-2.3) 		1.3
(-3) 		4.5
(0.3) 		1.6
(-2.7) 		5.6
(1.4) 		7.1
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191668_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15119
Sequence Type Exemplar sequence
Representative Public ID H05B21.2 NCBI
Target Description H05B21.2 /REP_DB=WormBase Gene ID /WP=CE17952 /GEN=srh-248 /TR=O61969 /GB=AAC17763.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071402(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071402 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-248) (srh-248) mRNA, complete cds. 11/11 None
H05B21.2 ENSEMBL cdna:known chromosome:CEL140:V:2954231:2955269:-1 gene:H05B21.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2954230-2955269(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-248
Ensembl H05B21.2
Entrez Gene 186691 Entrez gene
SwissProt O61969 EMBL-EBI
WormBase CE17952 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC17763 Serpentine receptor, class h protein 248 [Caenorhabditis elegans] ref|NP_503803.1| Serpentine Receptor, class H family member (srh-248) [Caenorhabditis elegans] 1.0E-178
blast NP_503796 Serpentine Receptor, class H family member (srh-247) [Caenorhabditis elegans] gb|AAG23979.1| Serpentine receptor, class h protein 247 [Caenorhabditis elegans] 1.0E-131
blast AAC71178 Serpentine receptor, class h protein 250 [Caenorhabditis elegans] ref|NP_494681.1| Serpentine Receptor, class H family member (srh-250) [Caenorhabditis elegans] 3.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503803.1 7 15-37,58-75,90-112,133-152,195-217,237-259,274-293
H05B21.2 7 15-37,58-75,90-112,133-152,195-217,237-259,274-293
Sequence
Target Sequence 
>C. ELEGANS:191668_AT
tgatgtggattctcgtacccttttattttttgccgccattttttcaaattccggatcaag
aatcggctcgaattcatattcaaaaagaaattccgtgtttgaacctaactctggtgaaca
ataggaatctattcgtcctgtcgttaaattcaaatcttgttggatattgtgctataatgg
aaactgtggttattgtttctccaattatcctcttcttttttctcacactttatcaatttt
tcaaaatacgcgactctatgaagttctcttcgaaaagctatcagcttcagaaaagctttt
taatagccataactttgcaatcacttttatcatttgttttcattcttgtaccagttacta
ttgtgctttatggttttgttttctggtattataatcaagttcttaacaattttatgagca
tcatgttttcactttttggcttggaaacgtgtgttgtgatgattttagtgcacagacctt
acagggagtatgcactatctcttcttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATGTGGATTCTCGTACCCTTTTA 127 565 422 Antisense
AATTCCGTGTTTGAACCTAACTCTG 700 171 510 Antisense
GAATCTATTCGTCCTGTCGTTAAAT 298 329 546 Antisense
TATTGTTTCTCCAATTATCCTCTTC 400 665 612 Antisense
TACGCGACTCTATGAAGTTCTCTTC 678 647 668 Antisense
AATAGCCATAACTTTGCAATCACTT 288 173 723 Antisense
ATTTGTTTTCATTCTTGTACCAGTT 164 19 753 Antisense
GTACCAGTTACTATTGTGCTTTATG 42 459 769 Antisense
GTGCTTTATGGTTTTGTTTTCTGGT 580 479 784 Antisense
TGAGCATCATGTTTTCACTTTTTGG 405 569 836 Antisense
GGGAGTATGCACTATCTCTTCTTCT 573 495 905 Antisense
Sequence Source Affymetrix Proprietary Database