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[191658_at] Putative membrane protein

Description F14H3.2 /REP_DB=WormBase Gene ID /WP=CE15827 /TR=SW:O45363 /GB=CAB05483.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster 6grp_high1_10_10 > 87      4grp_high_8_8 > 21      6grp_total > 87      4grp_total > 18      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 66.7
(-3)
P 		106.1
(-1.3)
P 		97.3
(-1.7)
P 		206.4
(3)
P 		140
B 55.7
(-3)
P 		107.5
(3)
P 		61.3
(-2.4)
P 		83.3
(0.2)
P 		52
C 77.5
(-2)
P 		92.1
(-1.3)
P 		58.5
(-3)
A 		178.4
(3)
M 		120
avr 66.6
(-3) 		101.9
(-0.6) 		72.4
(-2.6) 		156
(3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 77.9
(0.8)
P 		40.6
(-3)
P 		64.5
(-0.5)
P 		81.8
(1.2)
P 		66
(-0.4)
P 		98.8
(3)
P 		58
B 74.4
(-0.5)
P 		54.1
(-2.6)
P 		51.5
(-2.9)
P 		50.2
(-3)
A 		108.2
(3)
P 		65.1
(-1.5)
P 		58
C 54.7
(-2.5)
P 		94.2
(1.7)
P 		63.5
(-1.6)
P 		79.7
(0.1)
P 		50.3
(-3)
P 		106.8
(3)
P 		57
avr 69
(-1.2) 		63
(-2.4) 		59.8
(-3) 		70.6
(-0.9) 		74.8
(-0) 		90.2
(3) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 24.3
(-1.9)
P 		23
(-3)
P 		25.4
(-1)
P 		30.2
(3)
P 		7
B 17.8
(-1.2)
P 		26.5
(3)
P 		14.2
(-3)
P 		17.8
(-1.2)
P 		12
C 25.6
(0.2)
P 		26.5
(0.8)
P 		20.4
(-3)
A 		30.1
(3)
M 		10
avr 22.6
(-0.4) 		25.3
(2.3) 		20
(-3) 		26
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 14.4
(-2.8)
P 		14.4
(-2.8)
P 		14.1
(-3)
P 		20.8
(1)
P 		19.9
(0.5)
P 		24.1
(3)
P 		10
B 25.9
(-0.4)
P 		18
(-1.9)
P 		12.5
(-3)
P 		13.3
(-2.8)
A 		43.9
(3)
P 		24.3
(-0.7)
P 		31
C 20.3
(-1.7)
P 		28.7
(0.8)
P 		16.2
(-2.9)
P 		16
(-3)
P 		16.3
(-2.9)
P 		35.8
(3)
P 		20
avr 20.2
(-0.4) 		20.4
(-0.3) 		14.3
(-3) 		16.7
(-2) 		26.7
(2.4) 		28.1
(3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191658_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13749
Sequence Type Exemplar sequence
Representative Public ID F14H3.2 NCBI
Target Description F14H3.2 /REP_DB=WormBase Gene ID /WP=CE15827 /TR=SW:O45363 /GB=CAB05483.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074635(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074635 NCBI Caenorhabditis elegans F14H3.2 (F14H3.2) mRNA, complete cds. 11/11 None
SNAP00000013819 ENSEMBL cdna:SNAP chromosome:CEL140:V:16052316:16054137:1 11/11 None
GENEFINDER00000013834 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16052316:16054137:1 11/11 None
F14H3.2 ENSEMBL cdna:known chromosome:CEL140:V:16052316:16054137:1 gene:F14H3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16052315-16054137(+) 100.0 100.0
Public Domain and Genome References
Gene Title Putative membrane protein
Ensembl F14H3.2
Entrez Gene 184489 Entrez gene
SwissProt O45363 EMBL-EBI
WormBase CE15827 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05483 Hypothetical protein F14H3.2 [Caenorhabditis elegans] ref|NP_507036.1| F14H3.2 [Caenorhabditis elegans] sp|O45363|YSWJ_CAEEL Hypothetical protein F14H3.2 in chromosome V 0.0
blast CAB05708 Hypothetical protein C49A1.3 [Caenorhabditis elegans] ref|NP_493482.1| C49A1.3 [Caenorhabditis elegans] 9.0E-85
blast CAB07337 Hypothetical protein C37A5.1 [Caenorhabditis elegans] ref|NP_493472.1| C37A5.1 [Caenorhabditis elegans] 6.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.3E-121
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507036.1 3 32-54,241-263,278-300
F14H3.2 3 32-54,241-263,278-300
SNAP00000013819 3 32-54,241-263,278-300
GENEFINDER00000013834 3 32-54,241-263,278-300
Sequence
Target Sequence 
>C. ELEGANS:191658_AT
aaaactgactcgctacgatctcatcccgattcctctggcatatccgcaagcggtgtttct
tgcagttagaatctactttttcttttgtctgttcacacgacaacatttggatttggagga
aaattgggcgttgtcccactggggcttcccacttctcacgacactccagttcattttctt
ggttggttgtatgaaagttgccgagattctgctaaaccctatgggacaagatgatgagaa
ttttgagtgcaactatgtcatggacaagaatcttttcgtggggctgactattgttagttc
agaacacactgagtgtcccgaattggaagaggtgattggagatgactatgtgccttggta
tccggatgactgtaagagtaaggaggagaagaatcaagaagaattgaaaaaatacttgga
gagtgttgacttccaagccgttaccagtagtgaccaaggtgaaaatgacgaagtatccac
aatgatgaaggttgagcaaaattctctacttgtgtgtggccgagagaaattttatgaagg
cggcggttttcgcaattcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAACTGACTCGCTACGATCTCATC 355 131 720 Antisense
CCTCTGGCATATCCGCAAGCGGTGT 542 265 751 Antisense
TCTACTTTTTCTTTTGTCTGTTCAC 45 615 791 Antisense
GAAAATTGGGCGTTGTCCCACTGGG 490 347 838 Antisense
GTTGCCGAGATTCTGCTAAACCCTA 417 439 916 Antisense
TCTTTTCGTGGGGCTGACTATTGTT 649 611 990 Antisense
ACACACTGAGTGTCCCGAATTGGAA 453 111 1023 Antisense
GACTATGTGCCTTGGTATCCGGATG 691 371 1063 Antisense
TGACTTCCAAGCCGTTACCAGTAGT 104 505 1146 Antisense
AAATTCTCTACTTGTGTGTGGCCGA 65 117 1218 Antisense
TGAAGGCGGCGGTTTTCGCAATTCG 230 573 1254 Antisense
Sequence Source Affymetrix Proprietary Database