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[191651_at]

Description ZC518.1 /REP_DB=WormBase Gene ID /WP=CE06601 /TR=SW:Q23369 /GB=CAA92989.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 22      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 71      6grp_total > 32      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 349.1
(3)
P 		176.1
(-3)
P 		220.7
(-1.5)
P 		241.3
(-0.7)
P 		173
B 379.7
(3)
P 		284.6
(-0.4)
P 		241.3
(-1.9)
P 		210.2
(-3)
P 		170
C 476.7
(3)
P 		292.6
(-2)
P 		257.5
(-3)
P 		356.8
(-0.3)
P 		219
avr 401.8
(3) 		251.1
(-2.6) 		239.8
(-3) 		269.4
(-1.9) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 474
1 2 3 4 5 6 range
A 223
(-1.8)
P 		586.9
(3)
P 		443.2
(1.1)
P 		347.3
(-0.2)
P 		259.3
(-1.3)
P 		133.7
(-3)
P 		453
B 411.3
(0.7)
P 		346.9
(-0.2)
P 		577.5
(3)
P 		295.1
(-0.9)
P 		346.5
(-0.2)
P 		147.7
(-3)
P 		430
C 608
(3)
P 		370.5
(-0.1)
P 		343.9
(-0.4)
P 		343.6
(-0.4)
P 		447.5
(0.9)
P 		147.4
(-3)
P 		461
avr 414.1
(2.2) 		434.8
(2.6) 		454.9
(3) 		328.7
(0.6) 		351.1
(1) 		142.9
(-3) 		312
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 123
1 2 3 4 range
A 127.1
(3)
P 		38.2
(-2.8)
P 		57.7
(-1.5)
P 		35.3
(-3)
P 		92
B 121.1
(3)
P 		70.3
(-1)
P 		55.9
(-2.1)
P 		44.8
(-3)
P 		76
C 157.8
(3)
P 		84.1
(-1.5)
P 		89.8
(-1.2)
P 		60.2
(-3)
P 		98
avr 135.3
(3) 		64.2
(-1.8) 		67.8
(-1.6) 		46.8
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 41.1
(-2.7)
P 		208.7
(3)
P 		96.5
(-0.8)
P 		88.1
(-1.1)
P 		78.2
(-1.4)
P 		32.6
(-3)
P 		176
B 143.1
(3)
P 		115.5
(1.1)
P 		140.2
(2.8)
P 		78.2
(-1.4)
P 		140.8
(2.8)
P 		55.1
(-3)
P 		88
C 225.4
(3)
P 		113
(-0.8)
P 		87.5
(-1.7)
P 		69.1
(-2.3)
P 		144.8
(0.3)
P 		49.3
(-3)
P 		176
avr 136.5
(2.4) 		145.7
(3) 		108.1
(0.7) 		78.5
(-1) 		121.3
(1.5) 		45.7
(-3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191651_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8516
Sequence Type Exemplar sequence
Representative Public ID ZC518.1 NCBI
Target Description ZC518.1 /REP_DB=WormBase Gene ID /WP=CE06601 /TR=SW:Q23369 /GB=CAA92989.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069952(11), NM_182302(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069952 NCBI Caenorhabditis elegans ZC518.1a (ZC518.1) mRNA, complete cds. 11/11 None
NM_182302 NCBI Caenorhabditis elegans ZC518.1b (ZC518.1) mRNA, complete cds. 10/11 None
ZC518.1a.2 ENSEMBL cdna:known chromosome:CEL140:IV:12349913:12355462:1 gene:ZC518.1 11/11 None
ZC518.1a.1 ENSEMBL cdna:known chromosome:CEL140:IV:12349915:12355683:1 gene:ZC518.1 11/11 None
ZC518.1b ENSEMBL cdna:known chromosome:CEL140:IV:12349967:12355671:1 gene:ZC518.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000038452 8/11 Cross Hyb Matching Probes None
GENEFINDER00000038459 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12349972-12355468(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZC518.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92989 Hypothetical protein ZC518.1a [Caenorhabditis elegans] ref|NP_502353.1| ZC518.1a [Caenorhabditis elegans] sp|Q23369|YHDI_CAEEL Hypothetical protein ZC518.1 in chromosome IV 0.0
blast CAE59843 Hypothetical protein CBG03316 [Caenorhabditis briggsae] 0.0
blast CAD56618 Hypothetical protein ZC518.1b [Caenorhabditis elegans] ref|NP_872102.1| ZC518.1b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502353.1 2 32-54,75-97
NP_872102.1 2 32-54,75-97
ZC518.1a.2 2 32-54,75-97
ZC518.1a.1 2 32-54,75-97
ZC518.1b 2 32-54,75-97
Sequence
Target Sequence 
>C. ELEGANS:191651_AT
gatcaccggtttgtcaattgtggacaccatgtggaagcacgatgataccggttactcgat
ggtcgaagagcatatggcaaaaacgccggctcagaagaaggatgaattttggggaattga
taagattgctcctttgtattctatggagtcggctgaaagatcagtacatccgttggtggg
aagcgcaagtaaaattaatttggtaaagaataagaaggaaatcgttatgactccgcacaa
gaataagctcagcgagctggatccttcagagcagaaaacgtatctccgccgggtaaatgt
atccgatcacaatgcaaaacacgcgaaacaacgtggacttgaaagagccaactcgccgga
taaatgcttgagcaaaatgagatcaagatcgaacggaaagttcagaacatctgcaaatgg
atcacaaaacggtgttgatctgtggacacgagctggtgacattgaaatgaacgttgccac
atcgaatccaaatcaagtgcatccacattcaattgccgtat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCACCGGTTTGTCAATTGTGGAC 445 415 954 Antisense
GGAAGCACGATGATACCGGTTACTC 237 531 986 Antisense
AAGATTGCTCCTTTGTATTCTATGG 596 155 1075 Antisense
AAAGATCAGTACATCCGTTGGTGGG 388 113 1109 Antisense
GAAATCGTTATGACTCCGCACAAGA 553 361 1171 Antisense
CAGAGCAGAAAACGTATCTCCGCCG 455 205 1220 Antisense
ATCTCCGCCGGGTAAATGTATCCGA 525 31 1235 Antisense
AAGAGCCAACTCGCCGGATAAATGC 649 151 1296 Antisense
GATCTGTGGACACGAGCTGGTGACA 257 419 1390 Antisense
ATGAACGTTGCCACATCGAATCCAA 126 45 1420 Antisense
GTGCATCCACATTCAATTGCCGTAT 361 481 1450 Antisense
Sequence Source Affymetrix Proprietary Database