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[191630_at] 7tm receptor

Description T23F11.5 /REP_DB=WormBase Gene ID /WP=CE02356 /GEN=srg-13 /TR=SW:P46567 /GB=CAA86460.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=7tm receptor
Cluster 6grp_total > 53      4grp_total > 05      6grp_cgc > 90      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 52.7
(-0.1)
M 		33.1
(-2)
A 		23.4
(-3)
A 		84.1
(3)
A 		61
B 38.7
(1)
A 		13.5
(-3)
A 		26.3
(-1)
A 		51.2
(3)
M 		38
C 55.7
(-1.6)
P 		27.5
(-3)
A 		28.4
(-3)
A 		149.9
(3)
P 		122
avr 49
(-0.9) 		24.7
(-3) 		26
(-2.9) 		95.1
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 28.5
(-1)
A 		7.8
(-3)
A 		41.8
(0.3)
P 		53.6
(1.5)
A 		69.4
(3)
P 		18
(-2)
A 		62
B 35.1
(1.7)
P 		41.3
(2.7)
P 		35.8
(1.9)
A 		4.4
(-3)
A 		20.2
(-0.6)
A 		43.2
(3)
P 		39
C 33.8
(-1.1)
A 		30
(-2)
A 		51.4
(3)
A 		43.3
(1.1)
A 		25.5
(-3)
A 		27.7
(-2.5)
P 		26
avr 32.5
(-0.8) 		26.4
(-3) 		43
(3) 		33.8
(-0.3) 		38.4
(1.3) 		29.6
(-1.8) 		17
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 19.2
(3)
M 		7.2
(-2.5)
A 		6.1
(-3)
A 		12.3
(-0.2)
A 		13
B 12.4
(3)
A 		3.3
(-3)
A 		6.1
(-1.2)
A 		10.9
(2)
M 		9
C 18.4
(0.6)
P 		7.9
(-3)
A 		9.9
(-2.3)
A 		25.3
(3)
P 		17
avr 16.7
(3) 		6.1
(-3) 		7.4
(-2.3) 		16.2
(2.7) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 5.2
(-2.2)
A 		2.8
(-3)
A 		9.1
(-0.9)
P 		13.6
(0.6)
A 		20.9
(3)
P 		4.4
(-2.5)
A 		18
B 12.2
(1.4)
P 		13.8
(2.1)
P 		8.7
(0)
A 		1.2
(-3)
A 		8.2
(-0.2)
A 		16.1
(3)
P 		15
C 12.5
(2.3)
A 		9.1
(-2)
A 		13.1
(3)
A 		8.7
(-2.5)
A 		8.3
(-3)
A 		9.3
(-1.8)
P 		5
avr 10
(-0.2) 		8.6
(-2) 		10.3
(0.2) 		7.8
(-3) 		12.5
(3) 		9.9
(-0.3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191630_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7892
Sequence Type Exemplar sequence
Representative Public ID T23F11.5 NCBI
Target Description T23F11.5 /REP_DB=WormBase Gene ID /WP=CE02356 /GEN=srg-13 /TR=SW:P46567 /GB=CAA86460.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=7tm receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065552(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065552 NCBI Caenorhabditis elegans Serpentine Receptor, class G (gamma) family member (srg-13) (srg-13) mRNA, complete cds. 11/11 None
GENEFINDER00000039549 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4672780:4674282:1 10/11 None
T23F11.5 ENSEMBL cdna:known chromosome:CEL140:III:4672212:4674127:1 gene:T23F11.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4672211-4674127(+) 100.0 100.0
Public Domain and Genome References
Gene Title 7tm receptor
Gene Symbol srg-13
Ensembl T23F11.5
Entrez Gene 191839 Entrez gene
SwissProt P46567 EMBL-EBI
WormBase CE02356 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86460 Hypothetical protein T23F11.5 [Caenorhabditis elegans] ref|NP_497953.1| Serpentine Receptor, class G (gamma) family member (srg-13) [Caenorhabditis elegans] sp|P46567|SRG13_CAEEL Serpentine receptor class gamma-13 (Protein srg-13) 1.0E-172
blast 2123261AN chemosensory receptor 1.0E-166
blast CAA86460 Hypothetical protein T23F11.5 [Caenorhabditis elegans] ref|NP_497953.1| Serpentine Receptor, class G (gamma) family member (srg-13) [Caenorhabditis elegans] sp|P46567|SRG13_CAEEL Serpentine receptor class gamma-13 (Protein srg-13) 1.0E-124
blast 2123261AN chemosensory receptor 1.0E-123
blast CAE71164 Hypothetical protein CBG18020 [Caenorhabditis briggsae] 2.0E-76
blast CAE71164 Hypothetical protein CBG18020 [Caenorhabditis briggsae] 8.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497953.1 7 35-57,87-109,131-153,160-182,209-231,244-266,276-298
T23F11.5 7 35-57,87-109,131-153,160-182,209-231,244-266,276-298
GENEFINDER00000039549 6 13-35,55-74,81-103,130-152,165-187,197-219
Sequence
Target Sequence 
>C. ELEGANS:191630_AT
catgtgtagtgtttcctgttggttatagtgcgatctggaaaagaattcttacaccgatta
tcattgtgctttttgttttacctattggaataatatggaatgttttaatatctcgagttt
atgcaaatccgtctttcggaggcttcagtgttaactacataaaattagtatcatgggcaa
gcttatcaaaattacatctgacatactttatagtttccctgattttaattatagtaatat
cgggtgtaactctctatgctcttctcatattaaaacatcggatcaaatctgctgaacaaa
ctttgactattgccacaatggttctctcacttgaattttcatttttatctgtgatacaga
tttactttgcgttcttctcttcgtcaacatctgaatggagacctttcctcctacgagtta
tgtattttacctatgatttgttgaatttttctacaactattatatttatatcatgtaatc
ctaagcttcgaaaaatgttgcttaaaaggatgcagagtagtgtgacagttggcagatcta
cttctaactccact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATGTGTAGTGTTTCCTGTTGGTTA 563 209 434 Antisense
TCGAGTTTATGCAAATCCGTCTTTC 548 601 546 Antisense
GTCTTTCGGAGGCTTCAGTGTTAAC 686 467 564 Antisense
GACATACTTTATAGTTTCCCTGATT 248 371 633 Antisense
ATATCGGGTGTAACTCTCTATGCTC 383 21 670 Antisense
ACTCTCTATGCTCTTCTCATATTAA 193 101 682 Antisense
GCCACAATGGTTCTCTCACTTGAAT 390 279 745 Antisense
ACAGATTTACTTTGCGTTCTTCTCT 560 107 789 Antisense
TTCTTCTCTTCGTCAACATCTGAAT 132 691 805 Antisense
TGAATGGAGACCTTTCCTCCTACGA 440 573 825 Antisense
TGGCAGATCTACTTCTAACTCCACT 685 545 963 Antisense
Sequence Source Affymetrix Proprietary Database