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[191622_s_at] L-isoaspartyl/D-aspartyl methyltransferase

Description C10F3.5 /REP_DB=WormBase Gene ID /WP=CE08070 /GEN=pcm-1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=L-isoaspartyl-D-aspartyl methyltransferase
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 83      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 72      6grp_total > 84      4grp_total > 03      6grp_highest_sub > 04      6grp_cgc > 77      6grp_high1_sub > 04      6grp_cgc_sub > 04_05_06      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 676
1 2 3 4 range
A 285.3
(-2.7)
P 		288.1
(-2.7)
P 		259.1
(-3)
P 		814.8
(3)
P 		556
B 324.9
(-1.9)
P 		214
(-3)
P 		310.3
(-2.1)
P 		837.4
(3)
P 		623
C 313.3
(-2.2)
P 		247.1
(-2.8)
P 		226.2
(-3)
P 		890.4
(3)
P 		664
avr 307.8
(-2.4) 		249.7
(-3) 		265.2
(-2.8) 		847.5
(3) 		598

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 281
1 2 3 4 5 6 range
A 272.2
(-3)
P 		304.9
(-2.2)
P 		374.4
(-0.4)
P 		507.1
(3)
P 		406.1
(0.4)
P 		421.5
(0.8)
P 		235
B 337.4
(-1.8)
P 		286.8
(-3)
P 		391.4
(-0.5)
P 		486.9
(1.8)
P 		535.9
(3)
P 		392.4
(-0.5)
P 		249
C 255.3
(-3)
P 		284.8
(-2.2)
P 		354.9
(-0.2)
P 		467.9
(3)
P 		384.1
(0.6)
P 		398.5
(1)
P 		213
avr 288.3
(-3) 		292.2
(-2.9) 		373.6
(-0.4) 		487.3
(3) 		442
(1.6) 		404.1
(0.5) 		199
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 103.9
(1.4)
P 		62.5
(-3)
P 		67.7
(-2.5)
P 		119.3
(3)
P 		57
B 103.6
(-0.6)
P 		52.9
(-3)
P 		71.9
(-2.1)
P 		178.4
(3)
P 		126
C 103.7
(-0.5)
P 		71
(-3)
P 		78.8
(-2.4)
P 		150.1
(3)
P 		79
avr 103.7
(-0.1) 		62.1
(-3) 		72.8
(-2.3) 		149.3
(3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 50.2
(-3)
P 		108.4
(1.5)
P 		81.5
(-0.6)
P 		128.6
(3)
P 		122.5
(2.5)
P 		102.6
(1)
P 		78
B 117.4
(-1.9)
P 		95.5
(-3)
P 		95
(-3)
P 		129
(-1.3)
P 		217.7
(3)
P 		146.3
(-0.5)
P 		123
C 94.6
(-2)
P 		86.9
(-3)
P 		90.3
(-2.6)
P 		94.1
(-2.1)
P 		124.3
(1.8)
P 		133.4
(3)
P 		47
avr 87.4
(-3) 		96.9
(-2.2) 		88.9
(-2.9) 		117.2
(-0.3) 		154.8
(3) 		127.4
(0.6) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191622_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11287
Sequence Type Exemplar sequence
Representative Public ID C10F3.5 NCBI
Target Description C10F3.5 /REP_DB=WormBase Gene ID /WP=CE08070 /GEN=pcm-1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=L-isoaspartyl-D-aspartyl methyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072150(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072150 NCBI Caenorhabditis elegans Protein Carboxymethyltransferase family member (pcm-1) (pcm-1) mRNA, complete cds. 11/11 None
SNAP00000029254 ENSEMBL cdna:SNAP chromosome:CEL140:V:5964391:5966361:-1 10/11 None
GENEFINDER00000029267 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5963304:5966298:-1 11/11 None
C10F3.5 ENSEMBL cdna:known chromosome:CEL140:V:5963179:5966359:-1 gene:C10F3.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5963303-5966298(-) 100.0 100.0
Public Domain and Genome References
Gene Title L-isoaspartyl/D-aspartyl methyltransferase
Gene Symbol pcm-1
Ensembl C10F3.5
Entrez Gene 178981 Entrez gene
SwissProt Q27873 EMBL-EBI
WormBase CE08070 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1589792_AT similar to Protein-L-isoaspartate(D-aspartate) O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl/D-aspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) cfa
CANINE:1588856_AT similar to Protein-L-isoaspartate(D-aspartate) O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl/D-aspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) cfa
CANINE:1584641_S_AT similar to Protein-L-isoaspartate(D-aspartate) O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl/D-aspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) cfa
CANINE_2:CFA.3996.1.A1_S_AT similar to Protein-L-isoaspartate(D-aspartate) O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl/D-aspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) cfa
CANINE_2:CFAAFFX.1550.1.S1_AT similar to Protein-L-isoaspartate(D-aspartate) O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl/D-aspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) cfa
CHICKEN:GGA.15889.1.S1_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase gga
CHICKEN:GGA.16623.2.S1_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase gga
HG-U133A:208857_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133_PLUS_2:208857_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133A_2:208857_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133_PLUS_2:205202_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133A:205202_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-FOCUS:205202_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133A_2:205202_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U95AV2:37738_G_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U95AV2:37736_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133A:210156_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133_PLUS_2:210156_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U133A_2:210156_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
U133_X3P:G4885538_3P_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
U133_X3P:G180636_3P_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
U133_X3P:G1332402_3P_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HG-U95AV2:37737_AT Protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
HUGENEFL:D25547_AT Protein-L-isoaspartate (D-aspartate) O-methyltransferase hs
MU11KSUBA:AA543694_I_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MU11KSUBA:D38023_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MU11KSUBA:AA543694_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MU11KSUBA:AA543694_R_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MG-U74AV2:94348_F_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MG-U74AV2:95512_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MG-U74AV2:94347_I_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MG-U74BV2:164600_I_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MG-U74BV2:164446_R_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MG-U74CV2:169033_R_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOE430A:1422665_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430A_2:1422665_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430_2:1422665_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOE430A:1431085_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430A_2:1431085_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430_2:1431085_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOE430A:1456604_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430A_2:1456604_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430_2:1456604_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOE430A:1431086_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430A_2:1431086_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MOUSE430_2:1431086_S_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 mm
MU19KSUBB:TC32178_AT Protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 (Pcmt1), mRNA mm
MU19KSUBC:TC36321_AT Protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 (Pcmt1), mRNA mm
MU19KSUBC:TC36321_G_AT Protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 (Pcmt1), mRNA mm
MU19KSUBC:TC39416_AT Protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 (Pcmt1), mRNA mm
RG-U34A:M26686_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RG-U34A:M26686_G_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RG-U34B:RC_AA849797_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RG-U34C:RC_AI104517_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RAE230A:1371966_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RAT230_2:1371966_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RAE230A:1387258_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RAT230_2:1387258_A_AT protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
RG-U34A:RC_AI178024_AT Protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4719 protein-L-isoaspartate (D-aspartate) O-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB69887 Protein carboxymethyltransferase protein 1 [Caenorhabditis elegans] ref|NP_504551.3| Protein Carboxymethyltransferase family member (pcm-1) [Caenorhabditis elegans] sp|Q27873|PIMT_CAEEL Protein-L-isoaspartate O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) gb|AAB60240.1| protein-L-isoaspartate (D-aspartate) O-methyltransferase gb|AAA82166.1| protein-L-isoaspartate (D-aspartate) O-methyltransferase 1.0E-126
blast CAE64536 Hypothetical protein CBG09278 [Caenorhabditis briggsae] 1.0E-107
blast AAB69887 Protein carboxymethyltransferase protein 1 [Caenorhabditis elegans] ref|NP_504551.3| Protein Carboxymethyltransferase family member (pcm-1) [Caenorhabditis elegans] sp|Q27873|PIMT_CAEEL Protein-L-isoaspartate O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) gb|AAB60240.1| protein-L-isoaspartate (D-aspartate) O-methyltransferase gb|AAA82166.1| protein-L-isoaspartate (D-aspartate) O-methyltransferase 3.0E-93
blast CAE64536 Hypothetical protein CBG09278 [Caenorhabditis briggsae] 1.0E-78
blast NP_571540 l-isoaspartyl protein carboxyl methyltransferase [Danio rerio] gb|AAH75735.1| L-isoaspartyl protein carboxyl methyltransferase [Danio rerio] gb|AAA96020.1| L-isoaspartate (D-aspartate) O-methyltransferase [Danio rerio] sp|Q92047|PIMT_BRARE Protein-L-isoaspartate(D-aspartate) O-methyltransferase (Protein-beta-aspartate methyltransferase) (PIMT) (Protein L-isoaspartyl/D-aspartyl methyltransferase) (L-isoaspartyl protein carboxyl methyltransferase) 4.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR000682 EMBL-EBI Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1.0E-126
Pfam IPR000682 EMBL-EBI Protein-L-isoaspartate(D-aspartate) O-methyltransferase 2.1E-106
Sequence
Target Sequence 
>C. ELEGANS:191622_S_AT
ttgccagtcaaagagcctacgatgcgatgaaatccgttgatcgtggagattttgctccga
gagctccttatgaagacgctcctcaacgaattggatataatgcaacggtttctgcacctc
acatgcacgcagctgctctcgactacctacaaaaccacctggttgccggtgccaaagctc
ttgacgttggctcaggaagtggatatttgacagtttgtatggcaatgatggttggccgaa
atggcacggttgttggaattgaacatatgccacagctggtcgaattaagcgagaaaaata
tcagaaagcatcacagtgaacaattggaacgtggaaatgttatcattattgagggagacg
gtcgtcaaggattcgcggaaaaggctccatacaatgcaattcacgtgggagcagcttcaa
aaggagtccccaaagcgctcacggatcaactggct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCCAGTCAAAGAGCCTACGATGC 170 689 80 Antisense
CTCCGAGAGCTCCTTATGAAGACGC 164 235 134 Antisense
GAAGACGCTCCTCAACGAATTGGAT 74 339 151 Antisense
TAATGCAACGGTTTCTGCACCTCAC 239 637 177 Antisense
GACTACCTACAAAACCACCTGGTTG 248 335 220 Antisense
AAGCTCTTGACGTTGGCTCAGGAAG 200 149 254 Antisense
GAACATATGCCACAGCTGGTCGAAT 522 347 340 Antisense
GGATTCGCGGAAAAGGCTCCATACA 569 507 448 Antisense
GCAATTCACGTGGGAGCAGCTTCAA 58 323 475 Antisense
GAGCAGCTTCAAAAGGAGTCCCCAA 649 389 488 Antisense
CAAAGCGCTCACGGATCAACTGGCT 106 193 510 Antisense
Sequence Source Affymetrix Proprietary Database