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[191616_at] Putative membrane protein

Description F32G8.4 /REP_DB=WormBase Gene ID /WP=CE23700 /TR=SW:Q19978 /GB=CAA96648.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 41      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 25      6grp_total > 41      4grp_total > 52      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1966
1 2 3 4 range
A 3542.7
(1.7)
P 		3984.5
(3)
P 		2789.9
(-0.6)
P 		2018.4
(-3)
P 		1966
B 3580.4
(3)
P 		3347.8
(2)
P 		3211.2
(1.4)
P 		2232.7
(-3)
P 		1348
C 3338
(2.5)
P 		3440.7
(3)
P 		2950.1
(0.6)
P 		2225.7
(-3)
P 		1215
avr 3487
(2.6) 		3591
(3) 		2983.7
(0.5) 		2158.9
(-3) 		1432

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5602
1 2 3 4 5 6 range
A 4013
(0.2)
P 		4656.2
(1.4)
P 		5571.5
(3)
P 		4921.6
(1.8)
P 		3519.4
(-0.7)
P 		2235.4
(-3)
P 		3336
B 4405.4
(-1.5)
P 		4918.4
(-0.8)
P 		7837.3
(3)
P 		4771.2
(-1)
P 		3575.1
(-2.6)
P 		3229.6
(-3)
P 		4608
C 4157.5
(-0.3)
P 		4853.4
(1.1)
P 		5557.3
(2.5)
P 		5784.6
(3)
P 		3005
(-2.6)
P 		2786.9
(-3)
P 		2998
avr 4192
(-0.6) 		4809.3
(0.5) 		6322
(3) 		5159.1
(1) 		3366.5
(-2) 		2750.6
(-3) 		3571
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 994
1 2 3 4 range
A 1289.9
(3)
P 		864.9
(0.4)
P 		729.3
(-0.4)
P 		295.5
(-3)
P 		994
B 1141.8
(3)
P 		826.8
(0.2)
P 		744.1
(-0.6)
P 		475.8
(-3)
P 		666
C 1105
(3)
P 		989.2
(2)
P 		1028.2
(2.4)
P 		375.3
(-3)
P 		730
avr 1178.9
(3) 		893.6
(0.9) 		833.9
(0.4) 		382.2
(-3) 		797

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1359
1 2 3 4 5 6 range
A 739.8
(-1.9)
P 		1656
(3)
P 		1213.4
(0.6)
P 		1248.2
(0.8)
P 		1062
(-0.2)
P 		544.3
(-3)
P 		1112
B 1532.8
(-0.2)
P 		1637.1
(0.7)
P 		1903.1
(3)
P 		1263.7
(-2.5)
P 		1452.5
(-0.9)
P 		1204.3
(-3)
P 		699
C 1541
(3)
P 		1480.9
(2.4)
P 		1414.4
(1.8)
P 		1163.3
(-0.7)
P 		972.4
(-2.6)
P 		932.9
(-3)
P 		608
avr 1271.2
(0.2) 		1591.3
(3) 		1510.3
(2.3) 		1225.1
(-0.2) 		1162.3
(-0.7) 		893.8
(-3) 		698
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191616_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11832
Sequence Type Exemplar sequence
Representative Public ID F32G8.4 NCBI
Target Description F32G8.4 /REP_DB=WormBase Gene ID /WP=CE23700 /TR=SW:Q19978 /GB=CAA96648.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073307(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073307 NCBI Caenorhabditis elegans F32G8.4 (F32G8.4) mRNA, complete cds. 11/11 None
SNAP00000017272 ENSEMBL cdna:SNAP chromosome:CEL140:V:10560586:10563129:1 11/11 None
GENEFINDER00000017277 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10560586:10563216:1 11/11 None
F32G8.4 ENSEMBL cdna:known chromosome:CEL140:V:10560558:10563378:1 gene:F32G8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10560585-10563216(+) 100.0 100.0
Public Domain and Genome References
Gene Title Putative membrane protein
Ensembl F32G8.4
Entrez Gene 179471 Entrez gene
SwissProt Q19978 EMBL-EBI
WormBase CE37903 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA96648 Hypothetical protein F32G8.4 [Caenorhabditis elegans] ref|NP_505708.2| F32G8.4 [Caenorhabditis elegans] sp|Q19978|YV6L_CAEEL Hypothetical protein F32G8.4 in chromosome V 0.0
blast CAE64843 Hypothetical protein CBG09639 [Caenorhabditis briggsae] 0.0
blast H89192 protein F32G8.4 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505708.2 4 24-46,56-78,221-243,258-276
F32G8.4 4 24-46,56-78,221-243,258-276
SNAP00000017272 4 24-46,56-78,221-243,258-276
GENEFINDER00000017277 4 24-46,56-78,221-243,258-276
Sequence
Target Sequence 
>C. ELEGANS:191616_AT
ggatatgctcctcaaacaggtctacagttatcgtgatggatttgctatgctcttcgttta
cgattggatcaaaattccactcgtgtatactcaagttgttgccattgccacatatggtta
cttctttatttgtcttattggaagacagccaaaacttgatcaaagaagtatggaaaagga
aatcactattcttttccctattttcacaactttccaaatgcttttctaccttggttggtt
gaaggtcggacaattttcaattcgaaaaaaaatttcagagctgaactacgttcttgacag
aaatacagctattgctcacatgatggcttctgaactttctgatcaacttccatcaattgg
agctccaatggttccagcagttccacatacaagagcttctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATATGCTCCTCAAACAGGTCTAC 110 511 600 Antisense
ACAGGTCTACAGTTATCGTGATGGA 471 107 615 Antisense
ATTTGCTATGCTCTTCGTTTACGAT 474 13 639 Antisense
AATTCCACTCGTGTATACTCAAGTT 451 177 672 Antisense
ACTCAAGTTGTTGCCATTGCCACAT 328 99 688 Antisense
TGCCACATATGGTTACTTCTTTATT 398 587 705 Antisense
GAAATCACTATTCTTTTCCCTATTT 54 363 778 Antisense
CAACTTTCCAAATGCTTTTCTACCT 381 137 806 Antisense
GCTATTGCTCACATGATGGCTTCTG 131 305 907 Antisense
TTGGAGCTCCAATGGTTCCAGCAGT 422 709 956 Antisense
GCAGTTCCACATACAAGAGCTTCTT 273 313 976 Antisense
Sequence Source Affymetrix Proprietary Database