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[191594_at] Putative membrane protein

Description F32B6.9 /REP_DB=WormBase Gene ID /WP=CE09864 /TR=SW:O45435 /GB=CAB03043.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 42      6grp_total > 31      4grp_total > 22      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 703
1 2 3 4 range
A 1241
(3)
P 		785.7
(-1.3)
P 		606.4
(-3)
P 		1123.9
(1.9)
P 		635
B 1309
(3)
P 		730.3
(-2)
P 		611.2
(-3)
P 		1224
(2.3)
P 		698
C 1258.8
(3)
P 		772
(-1.5)
P 		615.8
(-3)
P 		1245.4
(2.9)
P 		643
avr 1269.6
(3) 		762.7
(-1.6) 		611.1
(-3) 		1197.8
(2.3) 		659

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 668
1 2 3 4 5 6 range
A 1165.7
(2.4)
P 		976.1
(0.7)
P 		1234
(3)
P 		998.5
(0.9)
P 		748.8
(-1.4)
P 		566.5
(-3)
P 		668
B 935.5
(-0.5)
P 		1085.3
(1.3)
P 		1232
(3)
P 		924.3
(-0.6)
P 		721.6
(-3)
P 		815
(-1.9)
P 		510
C 832.7
(-1.2)
P 		1175.5
(3)
P 		940.6
(0.2)
P 		848.4
(-1)
P 		680
(-3)
P 		826.5
(-1.2)
P 		496
avr 978
(0.7) 		1079
(2.2) 		1135.5
(3) 		923.7
(-0) 		716.8
(-3) 		736
(-2.7) 		419
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 310
1 2 3 4 range
A 451.8
(3)
P 		170.6
(-2.8)
P 		158.5
(-3)
P 		164.5
(-2.9)
P 		293
B 417.4
(3)
P 		180.4
(-2.2)
P 		141.6
(-3)
P 		260.8
(-0.4)
P 		276
C 416.7
(3)
P 		221.9
(-2.7)
P 		214.6
(-2.9)
P 		210
(-3)
P 		207
avr 428.6
(3) 		191
(-2.5) 		171.6
(-3) 		211.8
(-2.1) 		257

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 214.9
(-0.8)
P 		347.1
(3)
P 		268.7
(0.8)
P 		253.2
(0.3)
P 		225.9
(-0.5)
P 		137.9
(-3)
P 		209
B 325.5
(1.2)
P 		361.2
(3)
P 		299.2
(-0.2)
P 		244.8
(-3)
P 		293.2
(-0.5)
P 		303.9
(0)
P 		116
C 308.7
(1.4)
P 		358.7
(3)
P 		239.4
(-0.8)
P 		170.6
(-3)
P 		220.1
(-1.4)
P 		276.7
(0.4)
P 		188
avr 283
(-0.3) 		355.7
(3) 		269.1
(-0.9) 		222.9
(-3) 		246.4
(-1.9) 		239.5
(-2.3) 		133
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191594_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8975
Sequence Type Exemplar sequence
Representative Public ID F32B6.9 NCBI
Target Description F32B6.9 /REP_DB=WormBase Gene ID /WP=CE09864 /TR=SW:O45435 /GB=CAB03043.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069383(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069383 NCBI Caenorhabditis elegans F32B6.9 (F32B6.9) mRNA, complete cds. 11/11 None
SNAP00000041537 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9903884:9905693:1 11/11 None
GENEFINDER00000041538 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9903884:9905693:1 11/11 None
F32B6.9 ENSEMBL cdna:known chromosome:CEL140:IV:9903884:9905693:1 gene:F32B6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9903889-9905699(+) 100.0 100.0
Public Domain and Genome References
Gene Title Putative membrane protein
Ensembl F32B6.9
Entrez Gene 185198 Entrez gene
SwissProt O45435 EMBL-EBI
WormBase CE09864 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03043 Hypothetical protein F32B6.9 [Caenorhabditis elegans] ref|NP_501784.1| F32B6.9 [Caenorhabditis elegans] sp|O45435|YV4Q_CAEEL Hypothetical protein F32B6.9 in chromosome IV 0.0
blast CAE59969 Hypothetical protein CBG03460 [Caenorhabditis briggsae] 0.0
blast AAB09111 Hypothetical protein C43G2.4 [Caenorhabditis elegans] ref|NP_501000.2| C43G2.4 [Caenorhabditis elegans] sp|Q94175|YCEL_CAEEL Hypothetical protein C43G2.4 in chromosome IV 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501784.1 4 21-43,73-92,233-255,270-289
F32B6.9 4 21-43,73-92,233-255,270-289
SNAP00000041537 4 21-43,73-92,233-255,270-289
GENEFINDER00000041538 4 21-43,73-92,233-255,270-289
Sequence
Target Sequence 
>C. ELEGANS:191594_AT
gcttcccttttcaaccagtttgttggtgaacttcgaattttccgtcaatctcttcgtaag
ctgtattcatatgattgggtgtgtgttcctcttgtttatacacaagtcgctgctttggca
acctactctttcttctttttcacacttttcggccgtcaaccacttttcccagatattgaa
actggaaaagagcttgatctagttgtaccagtgtttacaatagttcaattcttattcttc
gttggatggtttaaggtcggacaagatttgatgcgaccctttggattagatgatgatgac
atcgaactgaactacattttggataggaatgttcgaatttcttttgctattgtaaatcaa
ctccaggaatcaccaattccggatttcgaaagtaacgacgataaactttggcacgaaatg
catccaccaaccaaagatggagaaacgtctccaataccaagaatcccacaacttccacat
tcaaagtattcgaaacaactttctgaacatccaccacgtcttcatgcttatgttcccata
gatgatggcaagggaagtatcaaggatttggagagtcatcacggatgcgtttccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTCCCTTTTCAACCAGTTTGTTG 632 295 628 Antisense
TTTTCCGTCAATCTCTTCGTAAGCT 384 675 665 Antisense
ATGATTGGGTGTGTGTTCCTCTTGT 17 47 698 Antisense
TACACAAGTCGCTGCTTTGGCAACC 420 641 726 Antisense
GTCAACCACTTTTCCCAGATATTGA 364 463 782 Antisense
GAGCTTGATCTAGTTGTACCAGTGT 369 389 817 Antisense
GATTTGATGCGACCCTTTGGATTAG 638 427 892 Antisense
ACGTCTCCAATACCAAGAATCCCAC 492 95 1072 Antisense
AAACAACTTTCTGAACATCCACCAC 308 129 1120 Antisense
CGTCTTCATGCTTATGTTCCCATAG 254 247 1144 Antisense
GAGAGTCATCACGGATGCGTTTCCC 343 395 1198 Antisense
Sequence Source Affymetrix Proprietary Database