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[191587_at] ubiquitin-conjugating enzyme

Description F26H9.7 /REP_DB=WormBase Gene ID /WP=CE09712 /TR=P91853 /GB=CAB04202.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 69      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 82      6grp_total > 79      4grp_total > 13      6grp_highest_sub > Cu      6grp_cgc > 29      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 702
1 2 3 4 range
A 384.8
(-0.6)
P 		226.5
(-2.1)
P 		137.2
(-3)
P 		763.9
(3)
P 		627
B 467.9
(-0.4)
P 		178.3
(-3)
P 		177.8
(-3)
P 		839.3
(3)
P 		662
C 488.4
(0)
P 		192.6
(-2.9)
P 		183.6
(-3)
P 		784.4
(3)
P 		601
avr 447
(-0.3) 		199.1
(-2.7) 		166.2
(-3) 		795.9
(3) 		630

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 261.6
(-3)
P 		333.7
(1.1)
P 		297.7
(-1)
P 		294
(-1.2)
P 		367.9
(3)
P 		333.5
(1.1)
P 		106
B 395.4
(-0.1)
P 		327.4
(-2.3)
P 		306.9
(-3)
P 		353.9
(-1.5)
P 		403.4
(0.1)
P 		492.1
(3)
P 		185
C 324.5
(-1)
P 		382.1
(0.3)
P 		231.9
(-3)
P 		275.8
(-2)
P 		338.9
(-0.6)
P 		503.4
(3)
P 		272
avr 327.2
(-1.2) 		347.7
(-0.5) 		278.8
(-3) 		307.9
(-1.9) 		370.1
(0.3) 		443
(3) 		164
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 140.1
(3)
P 		49.2
(-2.2)
P 		35.9
(-3)
P 		111.8
(1.4)
P 		104
B 149.2
(1.7)
P 		44
(-2.9)
P 		41.2
(-3)
P 		178.9
(3)
P 		138
C 161.7
(3)
P 		55.4
(-3)
P 		64
(-2.5)
P 		132.3
(1.3)
P 		106
avr 150.3
(3) 		49.5
(-2.9) 		47
(-3) 		141
(2.5) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 48.2
(-3)
P 		118.7
(3)
P 		64.8
(-1.6)
P 		74.6
(-0.8)
P 		111
(2.3)
P 		81.2
(-0.2)
P 		71
B 137.6
(0.5)
P 		109
(-1.1)
P 		74.5
(-3)
P 		93.7
(-1.9)
P 		163.9
(1.9)
P 		183.5
(3)
P 		109
C 120.3
(0.4)
P 		116.6
(0.2)
P 		59
(-2.8)
P 		55.5
(-3)
P 		109.7
(-0.1)
P 		168.5
(3)
P 		113
avr 102
(-0.2) 		114.8
(0.7) 		66.1
(-3) 		74.6
(-2.3) 		128.2
(1.8) 		144.4
(3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191587_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1058
Sequence Type Exemplar sequence
Representative Public ID F26H9.7 NCBI
Target Description F26H9.7 /REP_DB=WormBase Gene ID /WP=CE09712 /TR=P91853 /GB=CAB04202.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060081(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060081 NCBI Caenorhabditis elegans Ubiquitin E2 (conjugating enzyme) variant family member (uev-3) (uev-3) mRNA, complete cds. 11/11 None
GENEFINDER00000021661 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9318452:9320727:1 11/11 None
F26H9.7 ENSEMBL cdna:known chromosome:CEL140:I:9319534:9320727:1 gene:F26H9.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021654 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9319464-9320658(+) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin-conjugating enzyme
Gene Symbol uev-3
Ensembl F26H9.7
Entrez Gene 185003 Entrez gene
SwissProt P91853 EMBL-EBI
WormBase CE09712 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04202 Hypothetical protein F26H9.7 [Caenorhabditis elegans] ref|NP_492482.1| Ubiquitin E2 (conjugating enzyme) variant family member (uev-3) [Caenorhabditis elegans] 1.0E-125
blast CAE72332 Hypothetical protein CBG19477 [Caenorhabditis briggsae] 5.0E-39
blast CAE72332 Hypothetical protein CBG19477 [Caenorhabditis briggsae] 2.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.7E-4
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.7E-4
Sequence
Target Sequence 
>C. ELEGANS:191587_AT
gaaagtcgagaaatccctctgccagattgatattatcaccgaatttatgaatagaagtcg
acatttacaaggaaaaaaagtcaacggatgcataatgagaatcgacgaaacgaaacaatt
attatttcatgtaataattgatggacctgttggaagtatctatgaaggtggaacattttt
tgccgatattaacatccaaccatatcaaaatcattcattgattccaagagtttgttttca
cacatttatatttcatccgaatcttgggaaatatggaaactgggacatgcgtggaattca
atgggaacgacgatccaatttggaagttttatataattttattgtggaaggaatgcgaaa
tgtgaaatatgatattgaacgtagaaatcttgctcaattggaagatatgtcacaaccgaa
tatctcgcggcttgctaaagaggattggccgaaattcgagcgtacagcccgggaatttgt
aa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGTCGAGAAATCCCTCTGCCAG 11 361 78 Antisense
CCCTCTGCCAGATTGATATTATCAC 35 259 92 Antisense
ATGGACCTGTTGGAAGTATCTATGA 446 51 218 Antisense
AAGGTGGAACATTTTTTGCCGATAT 619 159 242 Antisense
TTGCCGATATTAACATCCAACCATA 490 701 257 Antisense
GATTCCAAGAGTTTGTTTTCACACA 45 435 297 Antisense
TTTTCACACATTTATATTTCATCCG 207 677 312 Antisense
AACTGGGACATGCGTGGAATTCAAT 340 141 355 Antisense
ACAACCGAATATCTCGCGGCTTGCT 294 113 489 Antisense
GGCCGAAATTCGAGCGTACAGCCCG 646 541 524 Antisense
GAGCGTACAGCCCGGGAATTTGTAA 265 387 535 Antisense
Sequence Source Affymetrix Proprietary Database