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[191581_at] Putative membrane protein

Description B0564.3 /REP_DB=WormBase Gene ID /WP=CE05177 /TR=SW:Q17528 /GB=CAA97765.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 42      6grp_highest_nonorm > 42      6grp_total > 20      4grp_total > 47      6grp_highest_sub > 02_03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1033
1 2 3 4 range
A 1145.9
(3)
P 		396
(-2.1)
A 		400.8
(-2.1)
A 		266.4
(-3)
A 		880
B 1138.4
(3)
P 		357.7
(-2.2)
A 		352.2
(-2.2)
A 		237.4
(-3)
A 		901
C 1269.9
(3)
P 		250.6
(-3)
A 		319.5
(-2.6)
M 		270.7
(-2.9)
A 		1019
avr 1184.7
(3) 		334.8
(-2.5) 		357.5
(-2.4) 		258.2
(-3) 		927

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1150
1 2 3 4 5 6 range
A 732
(-1)
P 		980.6
(0.3)
P 		1494.8
(3)
P 		822.4
(-0.5)
P 		1014.6
(0.5)
P 		345.1
(-3)
P 		1150
B 889.7
(0.7)
P 		1216
(3)
P 		1085.2
(2.1)
P 		1031
(1.7)
P 		961.8
(1.2)
P 		376.4
(-3)
P 		840
C 641.6
(-1.4)
P 		1197.8
(3)
P 		988.6
(1.3)
P 		725
(-0.8)
P 		925.8
(0.8)
P 		446
(-3)
P 		752
avr 754.4
(-0.3) 		1131.5
(2.6) 		1189.5
(3) 		859.5
(0.5) 		967.4
(1.3) 		389.2
(-3) 		800
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 381
1 2 3 4 range
A 417.2
(3)
P 		86
(-2.3)
A 		104.8
(-2)
A 		39
(-3)
A 		378
B 363
(3)
P 		88.4
(-2.3)
A 		81.6
(-2.4)
A 		50.6
(-3)
A 		312
C 420.4
(3)
P 		72.1
(-2.6)
A 		111.4
(-1.9)
M 		45.6
(-3)
A 		375
avr 400.2
(3) 		82.2
(-2.4) 		99.3
(-2.1) 		45.1
(-3) 		355

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 321
1 2 3 4 5 6 range
A 134.9
(-1.8)
P 		348.8
(3)
P 		325.5
(2.5)
P 		208.6
(-0.2)
P 		306.2
(2)
P 		84
(-3)
P 		265
B 309.6
(0.8)
P 		404.8
(3)
P 		263.5
(-0.2)
P 		273.1
(0)
P 		390.8
(2.7)
P 		140.4
(-3)
P 		264
C 237.8
(-0.5)
P 		365.5
(3)
P 		251.6
(-0.1)
P 		145.8
(-3)
P 		299.6
(1.2)
P 		149.3
(-2.9)
P 		220
avr 227.4
(-0.5) 		373
(3) 		280.2
(0.8) 		209.2
(-1) 		332.2
(2) 		124.6
(-3) 		248
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191581_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9091
Sequence Type Exemplar sequence
Representative Public ID B0564.3 NCBI
Target Description B0564.3 /REP_DB=WormBase Gene ID /WP=CE05177 /TR=SW:Q17528 /GB=CAA97765.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070122(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070122 NCBI Caenorhabditis elegans B0564.3 (B0564.3) mRNA, complete cds. 11/11 None
SNAP00000043351 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13100681:13109703:-1 10/11 None
GENEFINDER00000043367 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13108225:13109730:-1 11/11 None
B0564.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:13108110:13109769:-1 gene:B0564.3 11/11 None
B0564.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:13108110:13109749:-1 gene:B0564.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13108230-13109736(-) 100.0 100.0
Public Domain and Genome References
Gene Title Putative membrane protein
Ensembl B0564.3
Entrez Gene 178264 Entrez gene
SwissProt Q17528 EMBL-EBI
WormBase CE05177 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA97765 Hypothetical protein B0564.3 [Caenorhabditis elegans] ref|NP_502523.1| B0564.3 [Caenorhabditis elegans] sp|Q17528|YQE3_CAEEL Hypothetical protein B0564.3 in chromosome IV 0.0
blast CAE58653 Hypothetical protein CBG01822 [Caenorhabditis briggsae] 0.0
blast CAA97766 Hypothetical protein B0564.4 [Caenorhabditis elegans] ref|NP_502524.1| B0564.4 [Caenorhabditis elegans] sp|Q17529|YQE4_CAEEL Hypothetical protein B0564.4 in chromosome IV 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502523.1 4 31-53,73-90,241-263,275-292
B0564.3.1 4 31-53,73-90,241-263,275-292
B0564.3.2 4 31-53,73-90,241-263,275-292
SNAP00000043351 4 22-44,64-81,232-254,266-283
GENEFINDER00000043367 4 31-53,73-90,241-263,275-292
Sequence
Target Sequence 
>C. ELEGANS:191581_AT
ggtctgatgattgctgacaacccaatgagtacaccagaacttcggaaggatccattctac
gatgaagtagatgttccactgctatactcggaagaatcatctaacattccaaatcatcat
tatcacggttcagtgtccgaagtgagattggaacagaaaggaaacgcgcctgtaatgatg
atgcctcactctcagtccgcagctaatttgcgacgaatgatgtcgttcaagtcggtggat
gaggatgaaaaggatatcaacgcgttctcaatgtctcacgacgatgctagaatgaggaac
tggcgtgaagttagccttgattcgagctttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCTGATGATTGCTGACAACCCAA 11 503 976 Antisense
GACAACCCAATGAGTACACCAGAAC 116 365 991 Antisense
ACTTCGGAAGGATCCATTCTACGAT 467 101 1014 Antisense
GTAGATGTTCCACTGCTATACTCGG 365 457 1042 Antisense
CACGGTTCAGTGTCCGAAGTGAGAT 168 199 1099 Antisense
GTAATGATGATGCCTCACTCTCAGT 75 463 1147 Antisense
CACTCTCAGTCCGCAGCTAATTTGC 421 197 1162 Antisense
GATGTCGTTCAAGTCGGTGGATGAG 247 409 1194 Antisense
GATATCAACGCGTTCTCAATGTCTC 31 427 1228 Antisense
TCAATGTCTCACGACGATGCTAGAA 581 631 1243 Antisense
GAAGTTAGCCTTGATTCGAGCTTTT 36 337 1282 Antisense
Sequence Source Affymetrix Proprietary Database