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[191575_at] isoleucyl-tRNA synthetase

Description R11A8.6 /REP_DB=WormBase Gene ID /WP=CE06304 /TR=SW:Q21926 /GB=CAA94369.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=isoleucyl-tRNA synthetase
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 25      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 14      6grp_total > 25      4grp_total > 61      6grp_highest_sub > 02      6grp_cgc > 43      6grp_high1_sub > 01_02      6grp_cgc_sub > 02      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2080
1 2 3 4 range
A 4240.7
(0.7)
P 		4892.1
(3)
P 		4360.8
(1.2)
P 		3164.9
(-3)
P 		1727
B 4775.6
(3)
P 		3977.8
(-0.6)
P 		4665
(2.5)
P 		3437.1
(-3)
P 		1339
C 4245.7
(3)
P 		3437.8
(-0.4)
P 		4100.2
(2.4)
P 		2812.5
(-3)
P 		1433
avr 4420.7
(3) 		4102.6
(1.5) 		4375.3
(2.8) 		3138.2
(-3) 		1283

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1793
1 2 3 4 5 6 range
A 3907.8
(1.4)
P 		4353.3
(3)
P 		2680.8
(-3)
P 		3101.8
(-1.5)
P 		3116.9
(-1.4)
P 		3448.6
(-0.2)
P 		1673
B 4002.3
(1.5)
P 		4454.5
(3)
P 		2996.6
(-1.9)
P 		3422.7
(-0.5)
P 		2661.5
(-3)
P 		2874.7
(-2.3)
P 		1793
C 4153.5
(2)
P 		4431.3
(3)
P 		3858.3
(0.9)
P 		2922.6
(-2.6)
P 		2807.2
(-3)
P 		2804.9
(-3)
P 		1626
avr 4021.2
(1.5) 		4413
(3) 		3178.6
(-1.8) 		3149
(-1.9) 		2861.9
(-3) 		3042.7
(-2.3) 		1551
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1081
1 2 3 4 range
A 1544
(3)
P 		1062
(0.3)
P 		1140
(0.8)
P 		463.3
(-3)
P 		1081
B 1522.9
(3)
P 		982.4
(-1.1)
P 		1081
(-0.4)
P 		732.4
(-3)
P 		791
C 1405.4
(2.9)
P 		988.4
(0.2)
P 		1429.1
(3)
P 		474.2
(-3)
P 		955
avr 1490.8
(3) 		1010.9
(-0.1) 		1216.7
(1.2) 		556.6
(-3) 		934

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 965
1 2 3 4 5 6 range
A 720.4
(-2.2)
P 		1548.3
(3)
P 		583.8
(-3)
P 		786.7
(-1.7)
P 		940.6
(-0.8)
P 		839.6
(-1.4)
P 		965
B 1392.6
(2.3)
P 		1482.7
(3)
P 		727.7
(-3)
P 		906.5
(-1.6)
P 		1081.3
(-0.2)
P 		1072
(-0.3)
P 		755
C 1539.5
(3)
P 		1352.1
(1.8)
P 		982
(-0.5)
P 		587.7
(-3)
P 		908.4
(-1)
P 		939
(-0.8)
P 		952
avr 1217.5
(0.9) 		1461
(3) 		764.5
(-3) 		760.3
(-3) 		976.8
(-1.1) 		950.2
(-1.4) 		701
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191575_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8914
Sequence Type Exemplar sequence
Representative Public ID R11A8.6 NCBI
Target Description R11A8.6 /REP_DB=WormBase Gene ID /WP=CE06304 /TR=SW:Q21926 /GB=CAA94369.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=isoleucyl-tRNA synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069513(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069513 NCBI Caenorhabditis elegans Isoleucyl tRNA Synthetase family member (irs-1) (irs-1) mRNA, complete cds. 11/11 None
SNAP00000003246 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10368587:10372451:1 11/11 None
GENEFINDER00000003255 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10368587:10372451:1 11/11 None
R11A8.6 ENSEMBL cdna:known chromosome:CEL140:IV:10368137:10372821:1 gene:R11A8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10368592-10372457(+) 100.0 100.0
Public Domain and Genome References
Gene Title isoleucyl-tRNA synthetase
Gene Symbol irs-1
Ensembl R11A8.6
Entrez Gene 177926 Entrez gene
SwissProt Q21926 EMBL-EBI
WormBase CE06304 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18192_S_AT isoleucyl-tRNA synthetase, putative / isoleucine--tRNA ligase, putative at
ATGENOME1:18191_I_AT isoleucyl-tRNA synthetase, putative / isoleucine--tRNA ligase, putative at
ATH1-121501:255020_AT isoleucyl-tRNA synthetase, putative / isoleucine--tRNA ligase, putative at
CANINE_2:CFA.18144.1.S1_S_AT similar to Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase) (IleRS) (IRS) cfa
CANINE_2:CFA.18283.1.S1_S_AT similar to Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase) (IleRS) (IRS) cfa
CANINE_2:CFAAFFX.4352.1.S1_AT similar to Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase) (IleRS) (IRS) cfa
CANINE_2:CFAAFFX.4356.1.S1_S_AT similar to Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase) (IleRS) (IRS) cfa
CANINE_2:CFAAFFX.4366.1.S1_S_AT similar to Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase) (IleRS) (IRS) cfa
DROSOPHILA_2:1631106_S_AT Isoleucyl-tRNA synthetase dm
DROSGENOME1:154387_AT Isoleucyl-tRNA synthetase dm
CHICKEN:GGA.11126.1.S1_S_AT isoleucine-tRNA synthetase gga
CHICKEN:GGAAFFX.20489.1.S1_AT isoleucine-tRNA synthetase gga
CHICKEN:GGAAFFX.26315.1.S1_S_AT isoleucine-tRNA synthetase gga
HG-U133_PLUS_2:204744_S_AT isoleucine-tRNA synthetase hs
HG-U133A:204744_S_AT isoleucine-tRNA synthetase hs
HG-FOCUS:204744_S_AT isoleucine-tRNA synthetase hs
HG-U133A_2:204744_S_AT isoleucine-tRNA synthetase hs
HU35KSUBD:RC_T66318_F_AT isoleucine-tRNA synthetase hs
HUGENEFL:D28473_S_AT isoleucine-tRNA synthetase hs
U133_X3P:G7770071_3P_A_AT isoleucine-tRNA synthetase hs
HG-U95AV2:40827_AT isoleucine-tRNA synthetase hs
HG-U133_PLUS_2:238922_AT Isoleucine-tRNA synthetase hs
HG-U133B:238922_AT Isoleucine-tRNA synthetase hs
HG-U133_PLUS_2:239794_AT Isoleucine-tRNA synthetase hs
HG-U133B:239794_AT Isoleucine-tRNA synthetase hs
HG-U95D:70428_AT Isoleucine-tRNA synthetase hs
HG-U95E:81666_AT Isoleucine-tRNA synthetase hs
HG-U95D:83529_AT Isoleucine-tRNA synthetase hs
HU35KSUBC:RC_H84637_AT Isoleucine-tRNA synthetase hs
U133_X3P:HS.122517.0.A1_3P_AT Isoleucine-tRNA synthetase hs
U133_X3P:HS.157556.0.A1_3P_AT Isoleucine-tRNA synthetase hs
MU11KSUBB:MSA.18141.0_S_AT isoleucine-tRNA synthetase mm
MU11KSUBB:MSA.13756.0_S_AT isoleucine-tRNA synthetase mm
MU11KSUBB:MSA.22948.0_S_AT isoleucine-tRNA synthetase mm
MG-U74AV2:93752_AT isoleucine-tRNA synthetase mm
MOE430A:1452154_AT isoleucine-tRNA synthetase mm
MOUSE430A_2:1452154_AT isoleucine-tRNA synthetase mm
MOUSE430_2:1452154_AT isoleucine-tRNA synthetase mm
MOE430A:1426705_S_AT isoleucine-tRNA synthetase mm
MOUSE430A_2:1426705_S_AT isoleucine-tRNA synthetase mm
MOUSE430_2:1426705_S_AT isoleucine-tRNA synthetase mm
MU19KSUBA:TC24013_AT Isoleucine-tRNA synthetase, mRNA (cDNA clone IMAGE:3491909) mm
MU19KSUBB:TC27136_AT Isoleucine-tRNA synthetase, mRNA (cDNA clone IMAGE:3491909) mm
MU19KSUBC:TC35500_AT Isoleucine-tRNA synthetase, mRNA (cDNA clone IMAGE:3491909) mm
RG-U34B:RC_AA944278_AT isoleucine-tRNA synthetase (predicted) rn
RAE230A:1376418_A_AT isoleucine-tRNA synthetase (predicted) rn
RAT230_2:1376418_A_AT isoleucine-tRNA synthetase (predicted) rn
RAE230A:1386583_AT Isoleucine-tRNA synthetase (predicted) rn
RAT230_2:1386583_AT Isoleucine-tRNA synthetase (predicted) rn
YEAST_2:1779139_AT Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6428 isoleucyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4822 isoleucine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94369 Hypothetical protein R11A8.6 [Caenorhabditis elegans] ref|NP_501914.1| Isoleucyl tRNA Synthetase family member (irs-1) [Caenorhabditis elegans] sp|Q21926|SYI_CAEEL Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase) (IleRS) 0.0
blast CAE56141 Hypothetical protein CBG23754 [Caenorhabditis briggsae] 0.0
blast AAH56826 Isoleucine-tRNA synthetase [Danio rerio] ref|NP_956190.1| hypothetical protein LOC334393 [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191575_AT
agaagttgctgttagctacgcggctggaagtgatcaaggacatggatacaaaactcactc
ggatgcaaagaccattgtaatgattgatacaactgaagatgagtctcttattgaagaagg
actctgcagagaagtgacaaatcgtgttcaacgtcttcgcaaacaggctaaacttgtatc
tactgatactgctcatgttcatattgttgttagtccagctgattctcaactcgcaaaggt
tgttgctgccaaattgaatgacattgtatccgctactggaacacctatcaagttgggatc
accaccagccgctgcaaaggctccaactgccaccagcaagagtgcaattaaggattcaga
agttgagctttggttgttcgccgaaggtgacagtttcgatgggatcactgttgttgatgg
atcaaagaaagttcgagttcatgtgaaaacagaaaaagaacacctgaagggatatgctga
tcttctatatcacgttcgctctgctcttgatctctggaacggtaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGTTGCTGTTAGCTACGCGGCT 374 75 2823 Antisense
GCTACGCGGCTGGAAGTGATCAAGG 453 303 2837 Antisense
GGATACAAAACTCACTCGGATGCAA 46 509 2866 Antisense
GTTCAACGTCTTCGCAAACAGGCTA 278 445 2968 Antisense
ATCTACTGATACTGCTCATGTTCAT 90 33 3000 Antisense
CCAGCTGATTCTCAACTCGCAAAGG 159 273 3037 Antisense
GTATCCGCTACTGGAACACCTATCA 315 455 3088 Antisense
TCCAACTGCCACCAGCAAGAGTGCA 639 587 3144 Antisense
AGTTGAGCTTTGGTTGTTCGCCGAA 52 63 3183 Antisense
TGATCTTCTATATCACGTTCGCTCT 237 565 3300 Antisense
CTGCTCTTGATCTCTGGAACGGTAA 677 237 3323 Antisense
Sequence Source Affymetrix Proprietary Database