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[191574_at] G-protein coupled receptor

Description H10E21.2 /REP_DB=WormBase Gene ID /WP=CE19487 /TR=O76667 /GB=AAC26920.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_total > 91      4grp_total > 60      6grp_cgc > 51      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 94.4
(0)
A 		116.5
(2.3)
A 		123.6
(3)
A 		64.4
(-3)
A 		59
B 75.8
(3)
A 		51.7
(-3)
A 		64.6
(0.2)
A 		69
(1.3)
A 		24
C 96.5
(1.5)
A 		99.6
(1.7)
A 		121
(3)
A 		21.4
(-3)
A 		100
avr 88.9
(1.3) 		89.3
(1.4) 		103.1
(3) 		51.6
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 65.2
(-3)
A 		79.3
(-1.6)
A 		72.6
(-2.3)
A 		85.7
(-1)
A 		125.6
(3)
A 		91.2
(-0.4)
A 		60
B 104.4
(0.5)
A 		122.7
(1.7)
A 		91.1
(-0.5)
A 		140.8
(3)
A 		76.6
(-1.5)
A 		54.5
(-3)
A 		86
C 71.9
(1.9)
A 		81.6
(3)
A 		40.9
(-1.4)
A 		54.4
(0)
A 		51.1
(-0.3)
A 		26.4
(-3)
A 		55
avr 80.5
(0.7) 		94.5
(3) 		68.2
(-1.3) 		93.6
(2.9) 		84.4
(1.4) 		57.4
(-3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 34.4
(3)
A 		25.3
(0.8)
A 		32.3
(2.5)
A 		9.4
(-3)
A 		25
B 24.2
(3)
A 		12.8
(-3)
A 		15
(-1.8)
A 		14.7
(-2)
A 		11
C 31.9
(1.4)
A 		28.6
(0.9)
A 		42.2
(3)
A 		3.6
(-3)
A 		39
avr 30.2
(3) 		22.2
(0.7) 		29.8
(2.9) 		9.2
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 12
(-3)
A 		28.2
(0.8)
A 		15.8
(-2.1)
A 		21.7
(-0.8)
A 		37.9
(3)
A 		22.2
(-0.6)
A 		26
B 36.3
(1.7)
A 		40.8
(3)
A 		22.1
(-2.5)
A 		37.3
(2)
A 		31.1
(0.2)
A 		20.3
(-3)
A 		21
C 26.6
(3)
A 		24.9
(2.4)
A 		10.4
(-2.5)
A 		10.9
(-2.3)
A 		16.5
(-0.4)
A 		8.8
(-3)
A 		18
avr 25
(0.5) 		31.3
(3) 		16.1
(-3) 		23.3
(-0.2) 		28.5
(1.9) 		17.1
(-2.6) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191574_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8224
Sequence Type Exemplar sequence
Representative Public ID H10E21.2 NCBI
Target Description H10E21.2 /REP_DB=WormBase Gene ID /WP=CE19487 /TR=O76667 /GB=AAC26920.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064724
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000020272 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8856:12014:1 None
NM_064724 NCBI Caenorhabditis elegans H10E21.2 (H10E21.2) mRNA, complete cds. None
H10E21.2 ENSEMBL cdna:known chromosome:CEL140:III:8856:11940:1 gene:H10E21.2 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8855-12548(+) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Ensembl H10E21.2
Entrez Gene 186719 Entrez gene
SwissProt O76667 EMBL-EBI
WormBase CE28732 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK82900 Hypothetical protein H10E21.2 [Caenorhabditis elegans] ref|NP_497125.1| H10E21.2 [Caenorhabditis elegans] 0.0
blast T33403 hypothetical protein H10E21.2 - Caenorhabditis elegans 0.0
blast AAK82900 Hypothetical protein H10E21.2 [Caenorhabditis elegans] ref|NP_497125.1| H10E21.2 [Caenorhabditis elegans] 1.0E-117
blast T33403 hypothetical protein H10E21.2 - Caenorhabditis elegans 1.0E-105
blast CAE69520 Hypothetical protein CBG15729 [Caenorhabditis briggsae] 3.0E-76
blast CAE69520 Hypothetical protein CBG15729 [Caenorhabditis briggsae] 1.0E-60
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497125.1 5 15-37,50-72,152-174,209-231,246-265
H10E21.2 5 15-37,50-72,152-174,209-231,246-265
GENEFINDER00000020272 6 12-31,51-73,153-172,238-260,295-317,332-351
Sequence
Target Sequence 
>C. ELEGANS:191574_AT
aacgcccactcgactattatgacgagccagaggaagaagctgatgactataagagcgggg
gcttatcaggttgctacgcccactaaaatcttatttccagatgataactgcacagctcgg
gtttgtgcaaacttggctccgcgacgacaatactcttgctccaacactaatcaggccgcg
gtggagctcctccggcagtgtagtgcctaccgaaatccacgtggaaagtgtggtggagga
cgaggagacggagccggcggctccgcaaatttacttctttgcctgacccaaaagcacatc
attattattattaacaacattaatcccattccgcatcgcccgtttctcggcagtctccct
ggcttctgtctccataattttgtcgaggttgagcagctggagcaccagcttgtccagatc
ccaaatgtggaaaatatcgatttctctgtgattttgcacaaaatcattgccggcagtctg
gaaaaattcgattttcgaggtgatttgggttttggtaaaattttgaaaataaaaatttca
aaaaaactcacaaatatattgctcagcatgagcacaatggttccaaacctctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGCCCACTCGACTATTATGACGA 312 145 823 Antisense
GGGCTTATCAGGTTGCTACGCCCAC 578 491 881 Antisense
TGATAACTGCACAGCTCGGGTTTGT 699 563 924 Antisense
CAATACTCTTGCTCCAACACTAATC 464 181 970 Antisense
ACACTAATCAGGCCGCGGTGGAGCT 676 107 986 Antisense
CTCCGGCAGTGTAGTGCCTACCGAA 650 231 1012 Antisense
TTTACTTCTTTGCCTGACCCAAAAG 461 673 1092 Antisense
TAACAACATTAATCCCATTCCGCAT 250 639 1134 Antisense
GGCTTCTGTCTCCATAATTTTGTCG 102 537 1183 Antisense
GCTGGAGCACCAGCTTGTCCAGATC 45 295 1218 Antisense
GAGCACAATGGTTCCAAACCTCTCC 671 389 1392 Antisense
Sequence Source Affymetrix Proprietary Database