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[191572_s_at] elongation factor

Description F22B5.7 /REP_DB=WormBase Gene ID /WP=CE20707 /GEN=zyg-9 /TR=O61442 /GB=CAA90359.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=elongation factor
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 30      6grp_total > 27      4grp_total > 44      6grp_highest_sub > De      6grp_cgc > 14      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 450
1 2 3 4 range
A 1002.9
(1.2)
P 		1112
(3)
P 		785.5
(-2.3)
P 		743.1
(-3)
P 		369
B 1006.5
(3)
P 		942.3
(1.9)
P 		822.2
(-0.2)
P 		662.2
(-3)
P 		344
C 811.2
(-3)
P 		891
(3)
P 		818.5
(-2.5)
P 		881.6
(2.3)
P 		80
avr 940.2
(1.9) 		981.8
(3) 		808.7
(-1.7) 		762.3
(-3) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 719
1 2 3 4 5 6 range
A 1288.3
(3)
P 		1249.4
(2.6)
P 		667.6
(-3)
P 		665.6
(-3)
P 		737.3
(-2.3)
P 		905.2
(-0.7)
P 		623
B 1263.5
(3)
P 		918.4
(0)
P 		568.9
(-3)
P 		778.4
(-1.2)
P 		644.1
(-2.4)
P 		821.4
(-0.8)
P 		695
C 1071
(3)
P 		890.5
(0.6)
P 		862.7
(0.3)
P 		612
(-3)
P 		726.7
(-1.5)
P 		791.1
(-0.7)
P 		459
avr 1207.6
(3) 		1019.4
(0.8) 		699.7
(-2.8) 		685.3
(-3) 		702.7
(-2.8) 		839.2
(-1.2) 		522
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 256
1 2 3 4 range
A 365.1
(3)
P 		241.4
(0.1)
P 		205.3
(-0.7)
P 		108.8
(-3)
P 		256
B 321
(3)
P 		232.7
(0.1)
P 		190.5
(-1.4)
P 		141.1
(-3)
P 		180
C 268.5
(2.3)
P 		256.2
(1.7)
P 		285.3
(3)
P 		148.7
(-3)
P 		137
avr 318.2
(3) 		243.4
(0.6) 		227
(0) 		132.9
(-3) 		185

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 321
1 2 3 4 5 6 range
A 237.5
(-1.2)
P 		444.3
(3)
P 		145.4
(-3)
P 		168.8
(-2.5)
P 		222.5
(-1.5)
P 		220.4
(-1.5)
P 		299
B 439.6
(3)
P 		305.7
(0.3)
P 		138.1
(-3)
P 		206.2
(-1.6)
P 		261.7
(-0.5)
P 		306.3
(0.3)
P 		302
C 397
(3)
P 		271.7
(0.3)
P 		219.6
(-0.9)
P 		123.1
(-3)
P 		235.2
(-0.5)
P 		264.8
(0.1)
P 		274
avr 358
(3) 		340.6
(2.5) 		167.7
(-2.9) 		166
(-3) 		239.8
(-0.7) 		263.8
(0.1) 		192
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191572_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3891
Sequence Type Exemplar sequence
Representative Public ID F22B5.7 NCBI
Target Description F22B5.7 /REP_DB=WormBase Gene ID /WP=CE20707 /GEN=zyg-9 /TR=O61442 /GB=CAA90359.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=elongation factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063383(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063383 NCBI Caenorhabditis elegans ZYGote defective: embryonic lethal family member (zyg-9) (zyg-9) mRNA, complete cds. 11/11 A 
SNAP00000010406 ENSEMBL cdna:SNAP chromosome:CEL140:II:8457743:8464171:1 11/11 None
GENEFINDER00000010415 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8457743:8464171:1 11/11 None
F22B5.7 ENSEMBL cdna:known chromosome:CEL140:II:8457740:8464439:1 gene:F22B5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8457739-8464168(+) 100.0 100.0
Public Domain and Genome References
Gene Title elongation factor
Gene Symbol zyg-9
Ensembl F22B5.7
Entrez Gene 174350 Entrez gene
SwissProt O61442 EMBL-EBI
WormBase CE20707 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18421_AT microtubule organization 1 protein (MOR1) at
ATH1-121501:265800_AT microtubule organization 1 protein (MOR1) at
CANINE:1605459_AT similar to colonic and hepatic tumor over-expressed protein isoform a cfa
CANINE_2:CFAAFFX.14292.1.S1_S_AT similar to colonic and hepatic tumor over-expressed protein isoform a cfa
CANINE_2:CFAAFFX.14344.1.S1_S_AT similar to colonic and hepatic tumor over-expressed protein isoform a cfa
DROSOPHILA_2:1628172_AT mini spindles dm
DROSGENOME1:152738_AT mini spindles dm
CHICKEN:GGA.10463.1.S1_S_AT cytoskeleton associated protein 5 gga
CHICKEN:GGA.7921.1.S1_AT cytoskeleton associated protein 5 gga
CHICKEN:GGA.7921.1.S1_S_AT cytoskeleton associated protein 5 gga
CHICKEN:GGAAFFX.22534.1.S1_S_AT cytoskeleton associated protein 5 gga
CHICKEN:GGAAFFX.22534.3.S1_S_AT cytoskeleton associated protein 5 gga
HU35KSUBD:RC_AA496099_S_AT cytoskeleton associated protein 5 hs
HUGENEFL:D43948_AT cytoskeleton associated protein 5 hs
U133_X3P:HS2.76989.2.S1_3P_S_AT cytoskeleton associated protein 5 hs
U133_X3P:HS.76989.0.S1_3P_A_AT cytoskeleton associated protein 5 hs
HG-U133_PLUS_2:1555278_A_AT cytoskeleton associated protein 5 hs
HG-U133A_2:212832_S_AT cytoskeleton associated protein 5 hs
HG-U133_PLUS_2:212832_S_AT cytoskeleton associated protein 5 hs
HG-U133A:212832_S_AT cytoskeleton associated protein 5 hs
HG-U95AV2:37293_AT cytoskeleton associated protein 5 hs
MOE430B:1430647_AT cytoskeleton associated protein 5 mm
MOUSE430_2:1430647_AT cytoskeleton associated protein 5 mm
MU11KSUBA:AA214783_S_AT cytoskeleton associated protein 5 mm
MG-U74AV2:94243_AT cytoskeleton associated protein 5 mm
MOE430A:1437349_AT cytoskeleton associated protein 5 mm
MOUSE430A_2:1437349_AT cytoskeleton associated protein 5 mm
MOUSE430_2:1437349_AT cytoskeleton associated protein 5 mm
MU19KSUBB:TC34282_I_AT cytoskeleton associated protein 5 mm
MOE430B:1442271_AT Cytoskeleton associated protein 5, mRNA (cDNA clone IMAGE:4484883) mm
MOUSE430_2:1442271_AT Cytoskeleton associated protein 5, mRNA (cDNA clone IMAGE:4484883) mm
MU11KSUBA:AA204381_S_AT Cytoskeleton associated protein 5, mRNA (cDNA clone IMAGE:4484883) mm
RG-U34B:RC_AA946066_AT similar to colonic and hepatic tumor over-expressed protein isoform a rn
RAE230A:1388405_AT similar to colonic and hepatic tumor over-expressed protein isoform a rn
RAT230_2:1388405_AT similar to colonic and hepatic tumor over-expressed protein isoform a rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
922 spindle pole inferred from direct assay QuickGO AmiGO
5813 centrosome inferred from direct assay QuickGO AmiGO
5737 cytoplasm inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8017 microtubule binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE57635 Hypothetical protein CBG00620 [Caenorhabditis briggsae] 0.0
blast CAA90359 Hypothetical protein F22B5.7 [Caenorhabditis elegans] ref|NP_495784.1| ZYGote defective: embryonic lethal family member (zyg-9) [Caenorhabditis elegans] gb|AAC17865.1| ZYG-9 [Caenorhabditis elegans] 0.0
blast CAF93042 unnamed protein product [Tetraodon nigroviridis] 9.0E-56
Sequence
Target Sequence 
>C. ELEGANS:191572_S_AT
aggagacgtcgtcttggatgctgcccgtcgtctttctcgaccacatatgcacttgaccag
tatgatcaacaagattttacaaatgatgagagaacgaaaaattgatcctatcatgcctgg
aacattggaagccagaatgccacaagaggatgaagcagttgtagtgcgaagcggcgttca
agtttcgatcgacaatattctgcgtgatacctcgatggcagttaaacatattgagcaact
gaatattctcattgcatccagtgaccgatcttggaatgagtatatggaatatctcaaaaa
taatccaatgggagagctcatcaaggagctagtcggcgagtgttccagaaagaagaggat
tgactttaacctctctcatgtcgttaaatcgtcaatggcagttttcaaagccatggcagc
tactggtcctgttcaagaggaaggtagaataactccaacagatataaacagaatggatac
tatgattgttggaacacctctatctcgtggagacgcaaccatcactcgtgcgcgtggaaa
catgatccgtccaaagagaaccacattgagccgagacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGAGACGTCGTCTTGGATGCTGCC 213 57 3636 Antisense
TTTCTCGACCACATATGCACTTGAC 440 671 3668 Antisense
GAAGCGGCGTTCAAGTTTCGATCGA 676 343 3803 Antisense
TATTCTGCGTGATACCTCGATGGCA 168 663 3831 Antisense
TTCTCATTGCATCCAGTGACCGATC 297 691 3881 Antisense
GGAGCTAGTCGGCGAGTGTTCCAGA 384 519 3960 Antisense
GATTGACTTTAACCTCTCTCATGTC 14 435 3993 Antisense
CAAAGCCATGGCAGCTACTGGTCCT 669 189 4041 Antisense
GAACACCTCTATCTCGTGGAGACGC 414 351 4127 Antisense
CCATCACTCGTGCGCGTGGAAACAT 363 271 4154 Antisense
AGAGAACCACATTGAGCCGAGACCA 602 69 4190 Antisense
Sequence Source Affymetrix Proprietary Database