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[191562_at]

Description C25F6.2 /REP_DB=WormBase Gene ID /WP=CE26970 /GEN=dlg-1 /TR=Q18165 /GB=AAK39197.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanylate kinase
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      4grp_high_8_8 > 41      6grp_highest_nonorm > 50      6grp_total > 16      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 601
1 2 3 4 range
A 759.1
(3)
P 		359.4
(-2.5)
P 		325.9
(-2.9)
P 		319.1
(-3)
A 		440
B 794.3
(3)
P 		440.3
(-0.9)
P 		450.8
(-0.8)
P 		245.9
(-3)
P 		548
C 786
(3)
P 		510.5
(0.2)
P 		475.1
(-0.1)
P 		192.9
(-3)
A 		593
avr 779.8
(3) 		436.7
(-0.9) 		417.3
(-1.1) 		252.6
(-3) 		527

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1118
1 2 3 4 5 6 range
A 582.1
(0.3)
P 		872.3
(3)
P 		409.9
(-1.3)
P 		346.1
(-1.9)
P 		231.3
(-3)
P 		481.3
(-0.7)
P 		641
B 837.2
(3)
P 		736.7
(2)
P 		405.9
(-1.3)
P 		293.7
(-2.4)
P 		235.8
(-3)
P 		384.2
(-1.5)
P 		601
C 1348.8
(3)
P 		654
(-0.9)
P 		523.2
(-1.7)
P 		292.3
(-3)
P 		443.6
(-2.1)
P 		331.8
(-2.8)
P 		1057
avr 922.7
(3) 		754.3
(1.4) 		446.3
(-1.6) 		310.7
(-2.9) 		303.6
(-3) 		399.1
(-2.1) 		619
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 244
1 2 3 4 range
A 276.4
(3)
P 		78
(-2.2)
P 		85.2
(-2)
P 		46.7
(-3)
A 		230
B 253.3
(3)
P 		108.7
(-1.3)
P 		104.5
(-1.4)
P 		52.4
(-3)
P 		201
C 260.2
(3)
P 		146.8
(0)
P 		165.6
(0.5)
P 		32.5
(-3)
A 		228
avr 263.3
(3) 		111.2
(-1.2) 		118.4
(-1) 		43.9
(-3) 		219

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 441
1 2 3 4 5 6 range
A 107.3
(-2.1)
P 		310.2
(3)
P 		89.3
(-2.5)
P 		87.8
(-2.6)
P 		69.8
(-3)
P 		117.2
(-1.8)
P 		240
B 291.3
(3)
P 		245.2
(1.7)
P 		98.6
(-2.4)
P 		77.8
(-3)
P 		95.8
(-2.5)
P 		143.3
(-1.2)
P 		214
C 499.9
(3)
P 		199.6
(-1.1)
P 		133.2
(-2)
P 		58.8
(-3)
P 		143.5
(-1.8)
P 		111.1
(-2.3)
P 		441
avr 299.5
(3) 		251.7
(1.7) 		107
(-2.1) 		74.8
(-3) 		103
(-2.2) 		123.9
(-1.7) 		225
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191562_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17246
Sequence Type Exemplar sequence
Representative Public ID C25F6.2 NCBI
Target Description C25F6.2 /REP_DB=WormBase Gene ID /WP=CE26970 /GEN=dlg-1 /TR=Q18165 /GB=AAK39197.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanylate kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029260(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029260 NCBI Caenorhabditis elegans Drosophila Discs Large homolog family member (dlg-1) (dlg-1) mRNA, complete cds. 11/11 None
GENEFINDER00000000869 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5479117:5484390:1 10/11 None
SNAP00000000867 ENSEMBL cdna:SNAP chromosome:CEL140:X:5480745:5484090:1 10/11 None
C25F6.2a ENSEMBL cdna:known chromosome:CEL140:X:5479662:5484902:1 gene:C25F6.2 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180819 2 1 NM_001029260 174906_s_at (11), 191562_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5479433-5484858(+) 97.26 100.0
Public Domain and Genome References
Ensembl C25F6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39197 Drosophila discs large homolog protein 1, isoform a [Caenorhabditis elegans] emb|CAC35153.1| MAGUK protein DLG-1 [Caenorhabditis elegans] ref|NP_001024431.1| Drosophila Discs Large homolog family member (dlg-1) [Caenorhabditis elegans] gb|AAL01376.1| SAP97-like protein DLG-1 [Caenorhabditis elegans] 0.0
blast T15617 hypothetical protein C25F6.2 - Caenorhabditis elegans 0.0
blast CAE68757 Hypothetical protein CBG14691 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 7.1E-22
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 8.8E-21
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 7.4E-23
Pfam IPR001452 EMBL-EBI SH3 1.6E-7
Pfam IPR008144 EMBL-EBI Guanylate kinase 1.1E-41
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 7.1E-22
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 8.8E-21
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 7.4E-23
Pfam IPR001452 EMBL-EBI SH3 1.6E-7
Pfam IPR008144 EMBL-EBI Guanylate kinase 1.1E-41
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 7.1E-22
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 8.8E-21
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 7.4E-23
Pfam IPR001452 EMBL-EBI SH3 1.6E-7
Pfam IPR008144 EMBL-EBI Guanylate kinase 1.1E-41
Sequence
Target Sequence 
>C. ELEGANS:191562_AT
aactagcattcaaagcgtccgagatgtcgccaaccaaggaagacactgtattctggacgt
cagtggaaatgctatccgacgacttcaaagcaatgccaatattcaaccaatctcgatctt
catcaagcccagctctgcacaacagattcttgaattggacagccaacttgccaccaaccg
acaagacgatcgagcgatgagtggcgaagaagctcaagcgcagtattccagatgtcatag
aattgagcaaactttcggggatttgttcacgcaagaaatctcaaatacgccaatttgggt
gccacaaactctactactcgaactcagaaacctttcctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTAGCATTCAAAGCGTCCGAGAT 167 139 2835 Antisense
GTCCGAGATGTCGCCAACCAAGGAA 698 473 2851 Antisense
GAAGACACTGTATTCTGGACGTCAG 129 341 2873 Antisense
AAATGCTATCCGACGACTTCAAAGC 269 119 2901 Antisense
GCAATGCCAATATTCAACCAATCTC 163 323 2924 Antisense
AACCAATCTCGATCTTCATCAAGCC 81 149 2939 Antisense
GAATTGGACAGCCAACTTGCCACCA 461 323 2986 Antisense
TGCCACCAACCGACAAGACGATCGA 271 585 3003 Antisense
GCTCAAGCGCAGTATTCCAGATGTC 32 303 3046 Antisense
ACTTTCGGGGATTTGTTCACGCAAG 330 99 3085 Antisense
TACTCGAACTCAGAAACCTTTCCTT 210 645 3149 Antisense
Sequence Source Affymetrix Proprietary Database