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[191555_s_at] methionyl-tRNA synthetase

Description F58B3.5 /REP_DB=WormBase Gene ID /WP=CE06007 /TR=SW:Q20970 /GB=CAA97803.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=methionyl-tRNA synthetase
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 10      4grp_high_8_8 > 62      6grp_highest_nonorm > 76      6grp_total > 28      4grp_total > 56      6grp_highest_sub > Cu      6grp_cgc > 04      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 898
1 2 3 4 range
A 1563.6
(-1.8)
P 		1923.6
(1.3)
P 		2124.6
(3)
P 		1420.5
(-3)
P 		704
B 1447.6
(-2.1)
P 		1846.9
(0.8)
P 		2152.1
(3)
P 		1321.8
(-3)
P 		830
C 1435.8
(-1.1)
P 		1671.8
(1.3)
P 		1842.7
(3)
P 		1253.8
(-3)
P 		589
avr 1482.3
(-1.7) 		1814.1
(1.1) 		2039.8
(3) 		1332
(-3) 		708

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 893
1 2 3 4 5 6 range
A 1361.5
(2.3)
P 		1300.2
(1.6)
P 		894.1
(-3)
P 		989.7
(-1.9)
P 		1028.3
(-1.5)
P 		1427.5
(3)
P 		533
B 1310.3
(3)
P 		1231.6
(1.8)
P 		914.2
(-3)
P 		1125.7
(0.2)
P 		1037.6
(-1.1)
P 		912.5
(-3)
P 		398
C 1621.7
(3)
P 		1214.6
(0.3)
P 		1143.9
(-0.2)
P 		728.3
(-3)
P 		1252.5
(0.5)
P 		1000.4
(-1.2)
P 		893
avr 1431.2
(3) 		1248.8
(0.7) 		984.1
(-2.6) 		947.9
(-3) 		1106.1
(-1) 		1113.5
(-0.9) 		483
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 434
1 2 3 4 range
A 569.3
(3)
P 		417.6
(0.5)
P 		555.4
(2.8)
P 		207.9
(-3)
P 		361
B 461.6
(2)
P 		456.1
(1.8)
P 		498.7
(3)
P 		281.7
(-3)
P 		217
C 475.3
(0.7)
P 		480.7
(0.8)
P 		642.2
(3)
P 		211.4
(-3)
P 		431
avr 502.1
(1.9) 		451.5
(0.9) 		565.4
(3) 		233.7
(-3) 		332

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 455
1 2 3 4 5 6 range
A 251
(-1.7)
P 		462.4
(3)
P 		194.7
(-3)
P 		251
(-1.7)
P 		310.3
(-0.4)
P 		347.6
(0.4)
P 		268
B 455.9
(3)
P 		409.9
(1.8)
P 		222
(-3)
P 		298.2
(-1)
P 		421.6
(2.1)
P 		340.3
(0)
P 		234
C 601.1
(3)
P 		370.6
(-0)
P 		291.1
(-1.1)
P 		146.5
(-3)
P 		405.3
(0.4)
P 		334.9
(-0.5)
P 		455
avr 436
(3) 		414.3
(2.4) 		235.9
(-2.9) 		231.9
(-3) 		379.1
(1.3) 		340.9
(0.2) 		204
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191555_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8362
Sequence Type Exemplar sequence
Representative Public ID F58B3.5 NCBI
Target Description F58B3.5 /REP_DB=WormBase Gene ID /WP=CE06007 /TR=SW:Q20970 /GB=CAA97803.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=methionyl-tRNA synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069795(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069795 NCBI Caenorhabditis elegans Methionyl tRNA Synthetase family member (mrs-1) (mrs-1) mRNA, complete cds. 11/11 None
SNAP00000008574 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11632137:11633145:-1 11/11 None
GENEFINDER00000008588 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11632137:11635442:-1 11/11 None
F58B3.5 ENSEMBL cdna:known chromosome:CEL140:IV:11631920:11635457:-1 gene:F58B3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11632142-11635448(-) 100.0 100.0
Public Domain and Genome References
Gene Title methionyl-tRNA synthetase
Gene Symbol mrs-1
Ensembl F58B3.5
Entrez Gene 178089 Entrez gene
SwissProt Q20970 EMBL-EBI
WormBase CE06007 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:12659_AT methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative at
ATH1-121501:254734_AT methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative at
CANINE:1591673_AT similar to methionine-tRNA synthetase cfa
CANINE_2:CFA.18939.1.S1_S_AT similar to methionine-tRNA synthetase cfa
CANINE_2:CFAAFFX.1311.1.S1_AT similar to methionine-tRNA synthetase cfa
DROSOPHILA_2:1630677_AT dm
DROSGENOME1:147471_AT dm
HG-U133A:201475_X_AT methionine-tRNA synthetase hs
HG-U133_PLUS_2:201475_X_AT methionine-tRNA synthetase hs
HG-U133A_2:201475_X_AT methionine-tRNA synthetase hs
HG-U133A:213671_S_AT methionine-tRNA synthetase hs
HG-U133A_2:213671_S_AT methionine-tRNA synthetase hs
HG-FOCUS:213671_S_AT methionine-tRNA synthetase hs
HG-U133_PLUS_2:213671_S_AT methionine-tRNA synthetase hs
HUGENEFL:X94754_AT methionine-tRNA synthetase hs
U133_X3P:G4826825_3P_S_AT methionine-tRNA synthetase hs
HG-U95AV2:39342_AT methionine-tRNA synthetase hs
HG-U133A_2:213672_AT Methionine-tRNA synthetase hs
HG-U133_PLUS_2:213672_AT Methionine-tRNA synthetase hs
HG-U133A:213672_AT Methionine-tRNA synthetase hs
MU11KSUBA:AA408980_RC_S_AT methionine-tRNA synthetase mm
MU11KSUBB:MSA.17248.0_AT methionine-tRNA synthetase mm
MG-U74AV2:96138_AT methionine-tRNA synthetase mm
MOE430B:1455951_AT methionine-tRNA synthetase mm
MOUSE430_2:1455951_AT methionine-tRNA synthetase mm
RG-U34B:RC_AA924426_AT methionine-tRNA synthetase (predicted) rn
RG-U34B:RC_AA924426_G_AT methionine-tRNA synthetase (predicted) rn
RG-U34C:RC_AI231226_AT methionine-tRNA synthetase (predicted) rn
RAE230A:1389733_AT methionine-tRNA synthetase (predicted) rn
RAT230_2:1389733_AT methionine-tRNA synthetase (predicted) rn
RAE230B:1395363_AT methionine-tRNA synthetase (predicted) rn
RAT230_2:1395363_AT methionine-tRNA synthetase (predicted) rn
RG-U34C:RC_AI102047_AT Methionine-tRNA synthetase (predicted) rn
YEAST_2:1772317_AT Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6431 methionyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
49 tRNA binding inferred from electronic annotation QuickGO AmiGO
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4825 methionine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA97803 Hypothetical protein F58B3.5 [Caenorhabditis elegans] ref|NP_502196.1| Methionyl tRNA Synthetase family member (mrs-1) [Caenorhabditis elegans] sp|Q20970|SYM_CAEEL Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS) 0.0
blast CAE62085 Hypothetical protein CBG06108 [Caenorhabditis briggsae] 0.0
blast AAH57463 Mars protein [Danio rerio] 0.0
blast CAA97803 Hypothetical protein F58B3.5 [Caenorhabditis elegans] ref|NP_502196.1| Methionyl tRNA Synthetase family member (mrs-1) [Caenorhabditis elegans] sp|Q20970|SYM_CAEEL Methionyl-tRNA synthetase (Methionine--tRNA ligase) (MetRS) 1.0E-132
Protein Domains
Database ID Description E-Value
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 2.6E-26
Pfam IPR002547 EMBL-EBI t-RNA-binding region 2.8E-51
Pfam IPR002547 EMBL-EBI t-RNA-binding region 2.8E-51
Sequence
Target Sequence 
>C. ELEGANS:191555_S_AT
aagaaagaggctccttcaactccagcaccttccgagccgaagaaagctaaggagcagaag
aaaggaaagggtggggctgctgcggctccagtcgacgatacaatcgatgtgggaagactc
gatatgcgcgttggtcgaatcatcaagtgtgagaagcatcctgatgcagatgctctttac
gtggaacagatcgatgttggagagtcagccccgcgaactgtagtttctggactcgttcgt
cacgttccactcgatcaaatgcagaaccgccttgtcgttgtgctttgcaatctgaaacca
gcaaaaatgcgtggagtagagtcccgagccatggttatgtgtgcttcatcacctgacaaa
gttgagattatggaagttccggctgattccaaaccaggtactccagtcgtctgcccacca
tatacacacagacctgatgagcaattgaatccaaagaaaaagatctgggagacagttgca
gaggacttgaaggtttcggctgaaggcttcgcagagtggaaaggacaaccgcttctcatt
ggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAAAGAGGCTCCTTCAACTCCAG 462 153 2173 Antisense
GGGAAGACTCGATATGCGCGTTGGT 23 493 2283 Antisense
ATCGATGTTGGAGAGTCAGCCCCGC 502 33 2362 Antisense
TGTAGTTTCTGGACTCGTTCGTCAC 22 561 2391 Antisense
TCACGTTCCACTCGATCAAATGCAG 347 627 2412 Antisense
ATCAAATGCAGAACCGCCTTGTCGT 235 27 2426 Antisense
GTAGAGTCCCGAGCCATGGTTATGT 627 225 2488 Antisense
GGTTATGTGTGCTTCATCACCTGAC 659 505 2505 Antisense
GTTCCGGCTGATTCCAAACCAGGTA 18 441 2548 Antisense
AAGGTTTCGGCTGAAGGCTTCGCAG 527 129 2662 Antisense
AAAGGACAACCGCTTCTCATTGGAA 484 121 2692 Antisense
Sequence Source Affymetrix Proprietary Database