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[191552_s_at] MIPP repeats

Description R10H10.2 /REP_DB=WormBase Gene ID /WP=CE06293 /GEN=spe-26 /TR=SW:Q10579 /GB=CAA94610.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=MIPP repeats
Cluster 6grp_total > 87      4grp_total > 21      6grp_cgc > 58      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 28.9
(-1.7)
A 		8.1
(-3)
A 		9
(-2.9)
A 		101.3
(3)
A 		93
B 58.2
(3)
A 		35
(0.4)
A 		5.2
(-3)
A 		54.7
(2.6)
A 		53
C 46.1
(1.9)
A 		55.7
(3)
A 		5.5
(-3)
A 		43.7
(1.6)
A 		50
avr 44.4
(0.8) 		32.9
(-0.4) 		6.6
(-3) 		66.6
(3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 11.9
(-2.7)
A 		3.9
(-3)
A 		19.9
(-2.4)
A 		28.5
(-2.2)
A 		76.6
(-0.5)
A 		177.8
(3)
A 		174
B 39.6
(-2.1)
A 		41.7
(-2)
A 		57.5
(-1.2)
A 		22.3
(-3)
A 		75.8
(-0.3)
A 		142.5
(3)
A 		120
C 4.9
(-3)
A 		84.8
(2.7)
A 		6.9
(-2.9)
A 		75.1
(2)
A 		57.8
(0.7)
A 		89.7
(3)
P 		85
avr 18.8
(-3) 		43.5
(-1.7) 		28.1
(-2.5) 		42
(-1.8) 		70.1
(-0.4) 		136.7
(3) 		118
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 10.5
(1)
A 		1.8
(-3)
A 		2.4
(-2.7)
A 		14.8
(3)
A 		13
B 18.5
(3)
A 		8.6
(-0.4)
A 		1.2
(-3)
A 		11.7
(0.6)
A 		17
C 15.3
(2.7)
A 		16
(3)
A 		1.9
(-3)
A 		7.4
(-0.7)
A 		14
avr 14.8
(3) 		8.8
(0.2) 		1.8
(-3) 		11.3
(1.4) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 2.2
(-2.9)
A 		1.4
(-3)
A 		4.3
(-2.6)
A 		7.2
(-2.2)
A 		23.1
(0.1)
A 		43.3
(3)
A 		42
B 13.8
(-2)
A 		13.9
(-2)
A 		14
(-2)
A 		5.9
(-3)
A 		30.8
(0.2)
A 		53.1
(3)
A 		47
C 1.8
(-3)
A 		25.9
(2.1)
A 		1.8
(-3)
A 		15.1
(-0.2)
A 		18.7
(0.6)
A 		30
(3)
P 		28
avr 5.9
(-3) 		13.7
(-1.7) 		6.7
(-2.9) 		9.4
(-2.4) 		24.2
(0) 		42.1
(3) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191552_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10092
Sequence Type Exemplar sequence
Representative Public ID R10H10.2 NCBI
Target Description R10H10.2 /REP_DB=WormBase Gene ID /WP=CE06293 /GEN=spe-26 /TR=SW:Q10579 /GB=CAA94610.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=MIPP repeats
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069518(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069518 NCBI Caenorhabditis elegans defective SPErmatogenesis family member (spe-26) (spe-26) mRNA, complete cds. 11/11 None
SNAP00000020330 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10389595:10391882:-1 11/11 None
GENEFINDER00000020339 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10388531:10391882:-1 11/11 None
R10H10.2 ENSEMBL cdna:known chromosome:CEL140:IV:10389595:10391909:-1 gene:R10H10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10389600-10391888(-) 100.0 100.0
Public Domain and Genome References
Gene Title MIPP repeats
Gene Symbol spe-26
Ensembl R10H10.2
Entrez Gene 177929 Entrez gene
SwissProt Q10579 EMBL-EBI
WormBase CE06293 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94610 Hypothetical protein R10H10.2 [Caenorhabditis elegans] ref|NP_501919.1| defective SPErmatogenesis family member (spe-26) [Caenorhabditis elegans] sp|Q10579|SPE26_CAEEL Spermatocyte protein spe-26 gb|AAB60235.1| SPE-26 protein 0.0
blast CAE61762 Hypothetical protein CBG05722 [Caenorhabditis briggsae] 0.0
blast XP_687858 PREDICTED: similar to kelch-like 10 [Danio rerio] 4.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR006652 EMBL-EBI Kelch repeat 0.0016
Pfam IPR006652 EMBL-EBI Kelch repeat 5.5E-9
Pfam IPR006652 EMBL-EBI Kelch repeat 8.9E-12
Pfam IPR006652 EMBL-EBI Kelch repeat 2.0E-11
Pfam IPR006652 EMBL-EBI Kelch repeat 4.9E-5
Sequence
Target Sequence 
>C. ELEGANS:191552_S_AT
gagaggttttcgatccaaagaccaataaatggactgaaactgcaagcctatcagacatgc
gttccgactgtgctgctgaagtatttgagaatcaaatctacgttagtggtggtttcaatg
gtgacatgattcttgcgtctgttgaagtctacaatccaattggaaatgttttctctcgaa
ctgttgatctaccgtatccaataactggtcattgtcttctcaaccacggtaaccaattgc
ttattgttggaggatttgatggtgccgaacgacaaaacaagatttggatgtggcacagaa
ctggtgaatggcagcaacgaccggagaagttgatctatggaagatcaactagtgcagcat
gttcttacaaaggatggttattctcagtggctggatatacggagaaagtcgaagctactt
gtgaaatattgctccctgaaccgaatgcatctcgattcagttttattcctgatgttccta
gagccaaaagtgcattgaacgttttggtggcaccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGGTTTTCGATCCAAAGACCAA 130 393 1100 Antisense
CCTATCAGACATGCGTTCCGACTGT 457 269 1146 Antisense
GTTCCGACTGTGCTGCTGAAGTATT 100 441 1160 Antisense
GTGACATGATTCTTGCGTCTGTTGA 140 483 1220 Antisense
TTCTCTCGAACTGTTGATCTACCGT 31 693 1270 Antisense
ATTGTCTTCTCAACCACGGTAACCA 688 3 1310 Antisense
GTGAATGGCAGCAACGACCGGAGAA 31 479 1403 Antisense
GGATGGTTATTCTCAGTGGCTGGAT 230 513 1471 Antisense
CTCCCTGAACCGAATGCATCTCGAT 181 235 1531 Antisense
TATTCCTGATGTTCCTAGAGCCAAA 564 663 1563 Antisense
GCATTGAACGTTTTGGTGGCACCAA 162 307 1591 Antisense
Sequence Source Affymetrix Proprietary Database