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[191548_s_at]

Description C47E12.5 /REP_DB=WormBase Gene ID /WP=CE05449 /TR=Q27481 /GB=CAA93101.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=ubiquitin-activating enzyme
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 04      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 56      6grp_total > 15      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1742
1 2 3 4 range
A 2554.3
(1.9)
P 		2680.4
(2.5)
P 		2796.1
(3)
P 		1515
(-3)
P 		1281
B 2463.6
(0.7)
P 		2694.3
(1.7)
P 		2986.6
(3)
P 		1622.3
(-3)
P 		1364
C 2466.1
(1.5)
P 		2596.4
(2)
P 		2879.1
(3)
P 		1244.4
(-3)
P 		1635
avr 2494.7
(1.3) 		2657
(2) 		2887.3
(3) 		1460.6
(-3) 		1427

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1135
1 2 3 4 5 6 range
A 2195.4
(0.3)
P 		2594.3
(3)
P 		1910.5
(-1.6)
P 		1709.2
(-3)
P 		1941.4
(-1.4)
P 		2225.2
(0.5)
P 		885
B 2410.6
(3)
P 		2329.1
(2.3)
P 		2170.6
(1)
P 		1985.7
(-0.6)
P 		1743.8
(-2.7)
P 		1707.8
(-3)
P 		703
C 2638.6
(3)
P 		1928.4
(-0.8)
P 		2458.1
(2)
P 		1656.1
(-2.2)
P 		2353.4
(1.5)
P 		1503.5
(-3)
P 		1135
avr 2414.9
(3) 		2283.9
(1.8) 		2179.7
(0.8) 		1783.7
(-3) 		2012.9
(-0.8) 		1812.2
(-2.7) 		631
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 794
1 2 3 4 range
A 930
(3)
P 		581.8
(0)
P 		731
(1.3)
P 		221.8
(-3)
P 		708
B 785.6
(3)
P 		665.4
(1.4)
P 		692.1
(1.7)
P 		345.7
(-3)
P 		440
C 816.3
(1.6)
P 		746.5
(1.1)
P 		1003.5
(3)
P 		209.8
(-3)
P 		794
avr 844
(3) 		664.6
(1.2) 		808.9
(2.6) 		259.1
(-3) 		585

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 645
1 2 3 4 5 6 range
A 404.7
(-3)
P 		922.7
(3)
P 		416.1
(-2.9)
P 		433.5
(-2.7)
P 		585.8
(-0.9)
P 		541.8
(-1.4)
P 		518
B 838.8
(3)
P 		775.3
(1.8)
P 		527.1
(-3)
P 		525.9
(-3)
P 		708.5
(0.5)
P 		636.8
(-0.9)
P 		313
C 978
(3)
P 		588.4
(-0.6)
P 		625.6
(-0.3)
P 		333
(-3)
P 		761.5
(1)
P 		503.3
(-1.4)
P 		645
avr 740.5
(2.6) 		762.1
(3) 		522.9
(-1.3) 		430.8
(-3) 		685.3
(1.6) 		560.6
(-0.6) 		331
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191548_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9867
Sequence Type Exemplar sequence
Representative Public ID C47E12.5 NCBI
Target Description C47E12.5 /REP_DB=WormBase Gene ID /WP=CE05449 /TR=Q27481 /GB=CAA93101.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=ubiquitin-activating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069399(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069399 NCBI Caenorhabditis elegans UBA (human ubiquitin) related family member (uba-1) (uba-1) mRNA, complete cds. 11/11 None
SNAP00000021567 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9989904:9993357:-1 11/11 None
GENEFINDER00000021589 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9989904:9994895:-1 11/11 None
C47E12.5.2 ENSEMBL cdna:known chromosome:CEL140:IV:9989428:9994917:-1 gene:C47E12.5 11/11 A 
C47E12.5.3 ENSEMBL cdna:known chromosome:CEL140:IV:9989645:9995006:-1 gene:C47E12.5 11/11 A 
C47E12.5.1 ENSEMBL cdna:known chromosome:CEL140:IV:9989645:9994917:-1 gene:C47E12.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9989909-9994901(-) 100.0 100.0
Public Domain and Genome References
Ensembl C47E12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93101 Hypothetical protein C47E12.5a [Caenorhabditis elegans] ref|NP_501800.1| UBA (human ubiquitin) related family member (uba-1) [Caenorhabditis elegans] 0.0
blast CAJ30225 Hypothetical protein C47E12.5b [Caenorhabditis elegans] 0.0
blast CAE59952 Hypothetical protein CBG03440 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000127 EMBL-EBI Ubiquitin-activating enzyme repeat 2.8E-41
Pfam IPR000594 EMBL-EBI UBA/THIF-type NAD/FAD binding fold 3.1E-42
Pfam IPR000594 EMBL-EBI UBA/THIF-type NAD/FAD binding fold 3.9E-63
Pfam IPR000127 EMBL-EBI Ubiquitin-activating enzyme repeat 2.8E-41
Pfam IPR000594 EMBL-EBI UBA/THIF-type NAD/FAD binding fold 3.1E-42
Pfam IPR000594 EMBL-EBI UBA/THIF-type NAD/FAD binding fold 3.9E-63
Sequence
Target Sequence 
>C. ELEGANS:191548_S_AT
agttcatcacggctgcatcgaatctccgagccgaaaactatgatattcttccagctgata
gaatgagaaccaaacaaattgctggaaaaattattccagccatcgctaccaccaccgcag
cagttgccggtcttgtttgcatcgaactgtacaaggttgttgacgcaaacggtattccta
agacaccaatggagcgtttcaagaacactttcctaaatctgtcaatgccattcttctcct
ctgccgaaccaattggcgctccaaagaaaacttacatggaccgagagtttactctctggg
atagaattgacgttcaaggaccgctcacacttcaagaattcatcgacaatgtacaaaacc
aaactggtggatgcgaggtgtcgatgttatccgctggagcgtgtcttttgttctcgtttt
tcatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTCATCACGGCTGCATCGAATCT 647 63 2762 Antisense
GCATCGAATCTCCGAGCCGAAAACT 604 307 2776 Antisense
AAAATTATTCCAGCCATCGCTACCA 251 133 2848 Antisense
TTGCCGGTCTTGTTTGCATCGAACT 241 699 2885 Antisense
GACGCAAACGGTATTCCTAAGACAC 162 381 2923 Antisense
GGAGCGTTTCAAGAACACTTTCCTA 537 519 2952 Antisense
CACTTTCCTAAATCTGTCAATGCCA 251 197 2967 Antisense
AATTGACGTTCAAGGACCGCTCACA 552 175 3066 Antisense
GCGAGGTGTCGATGTTATCCGCTGG 562 289 3134 Antisense
ATCCGCTGGAGCGTGTCTTTTGTTC 18 39 3150 Antisense
TGTCTTTTGTTCTCGTTTTTCATGA 300 559 3163 Antisense
Sequence Source Affymetrix Proprietary Database