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[191544_s_at] cyctochrome c1 heme lyase

Description T06D8.6 /REP_DB=WormBase Gene ID /WP=CE02327 /TR=SW:P53703 /GB=CAA88969.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cyctochrome c1 heme lyase
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 68      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 77      6grp_total > 69      4grp_total > 09      6grp_highest_sub > Cu      6grp_cgc > 29      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2297
1 2 3 4 range
A 1244.9
(-3)
P 		1604.8
(-2)
P 		1592
(-2.1)
P 		3516.2
(3)
P 		2271
B 1411.3
(-3)
P 		1741.1
(-2)
P 		1799
(-1.8)
P 		3411.3
(3)
P 		2000
C 1219.7
(-3)
P 		1709.5
(-1.3)
P 		1441
(-2.2)
P 		2961.1
(3)
P 		1741
avr 1292
(-3) 		1685.1
(-1.8) 		1610.7
(-2) 		3296.2
(3) 		2004

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 696
1 2 3 4 5 6 range
A 1447.4
(3)
P 		1249.7
(0.4)
P 		1125.2
(-1.2)
P 		985.8
(-3)
P 		1275.9
(0.8)
P 		1313.7
(1.3)
P 		462
B 1131.3
(-3)
P 		1329.1
(-0.8)
P 		1277.5
(-1.4)
P 		1257.8
(-1.6)
P 		1295.8
(-1.2)
P 		1681.9
(3)
P 		551
C 1295.8
(-0.9)
P 		1342.9
(-0.4)
P 		1091.1
(-3)
P 		1141.8
(-2.5)
P 		1232.2
(-1.5)
P 		1673.6
(3)
P 		583
avr 1291.5
(-0.7) 		1307.2
(-0.5) 		1164.6
(-2.5) 		1128.5
(-3) 		1268
(-1) 		1556.4
(3) 		428
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 379
1 2 3 4 range
A 453.3
(0.8)
P 		348.4
(-3)
P 		416.2
(-0.6)
P 		514.7
(3)
P 		166
B 450.1
(-2.4)
P 		430
(-2.7)
P 		416.9
(-3)
P 		727
(3)
P 		310
C 403.7
(-3)
P 		491.5
(2.3)
P 		502.2
(3)
P 		499.3
(2.8)
P 		99
avr 435.7
(-2.5) 		423.3
(-3) 		445.1
(-2.2) 		580.3
(3) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 398
1 2 3 4 5 6 range
A 266.8
(-2.3)
P 		444.4
(3)
P 		245.1
(-3)
P 		250
(-2.9)
P 		385
(1.2)
P 		319.9
(-0.7)
P 		199
B 393.6
(-1.4)
P 		442.4
(-0.5)
P 		310.2
(-3)
P 		333.2
(-2.6)
P 		526.5
(1.1)
P 		627.2
(3)
P 		317
C 480.3
(1.5)
P 		409.8
(0.3)
P 		277.7
(-2.1)
P 		229.6
(-3)
P 		398.7
(0.1)
P 		560.3
(3)
P 		331
avr 380.2
(-0.2) 		432.2
(1.2) 		277.7
(-2.8) 		270.9
(-3) 		436.7
(1.3) 		502.5
(3) 		232
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191544_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3114
Sequence Type Exemplar sequence
Representative Public ID T06D8.6 NCBI
Target Description T06D8.6 /REP_DB=WormBase Gene ID /WP=CE02327 /TR=SW:P53703 /GB=CAA88969.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cyctochrome c1 heme lyase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064002(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064002 NCBI Caenorhabditis elegans T06D8.6 (T06D8.6) mRNA, complete cds. 11/11 None
SNAP00000001112 ENSEMBL cdna:SNAP chromosome:CEL140:II:11235243:11236280:-1 11/11 None
GENEFINDER00000001123 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11235243:11236280:-1 11/11 None
T06D8.6.2 ENSEMBL cdna:known chromosome:CEL140:II:11234969:11236293:-1 gene:T06D8.6 11/11 None
T06D8.6.1 ENSEMBL cdna:known chromosome:CEL140:II:11234969:11236356:-1 gene:T06D8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11235239-11236277(-) 100.0 100.0
Public Domain and Genome References
Gene Title cyctochrome c1 heme lyase
Gene Symbol cchl-1
Ensembl T06D8.6
Entrez Gene 174713 Entrez gene
SwissProt P53703 EMBL-EBI
WormBase CE02327 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1585361_X_AT similar to Cytochrome c-type heme lyase (CCHL) (Holocytochrome c-type synthase) cfa
CANINE:1605399_AT similar to Cytochrome c-type heme lyase (CCHL) (Holocytochrome c-type synthase) cfa
CANINE:1584682_S_AT similar to Cytochrome c-type heme lyase (CCHL) (Holocytochrome c-type synthase) cfa
CANINE_2:CFAAFFX.18006.1.S1_S_AT similar to Cytochrome c-type heme lyase (CCHL) (Holocytochrome c-type synthase) cfa
DROSOPHILA_2:1637165_AT dm
DROSGENOME1:153443_AT dm
CHICKEN:GGA.15865.1.S1_AT similar to Cytochrome c-type heme lyase (CCHL) (Holocytochrome c-type synthase) gga
CHICKEN:GGA.9645.1.S1_A_AT similar to Cytochrome c-type heme lyase (CCHL) (Holocytochrome c-type synthase) gga
HG-U95AV2:38943_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-U133_PLUS_2:203746_S_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-U133A:203746_S_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-U133A_2:203746_S_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HU35KSUBA:U36787_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HUGENEFL:U36787_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HU35KSUBB:RC_T89379_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
U133_X3P:G4885400_3P_A_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
U133_X3P:HS.211571.0.S2_3P_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-U133A_2:203745_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-FOCUS:203745_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-U133_PLUS_2:203745_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-U133A:203745_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
HG-U95E:90576_AT holocytochrome c synthase (cytochrome c heme-lyase) hs
MOE430A:1420889_AT holocytochrome c synthetase mm
MOUSE430A_2:1420889_AT holocytochrome c synthetase mm
MOUSE430_2:1420889_AT holocytochrome c synthetase mm
MU11KSUBB:MSA.3227.0_S_AT holocytochrome c synthetase mm
MG-U74AV2:102007_AT holocytochrome c synthetase mm
MG-U74AV2:160573_AT holocytochrome c synthetase mm
MOE430A:1420890_AT holocytochrome c synthetase mm
MOUSE430A_2:1420890_AT holocytochrome c synthetase mm
MOUSE430_2:1420890_AT holocytochrome c synthetase mm
MU19KSUBB:TC30605_AT Holocytochrome c synthetase (Hccs), mRNA mm
MU19KSUBB:TC30605_G_AT Holocytochrome c synthetase (Hccs), mRNA mm
MU19KSUBC:TC37421_AT Holocytochrome c synthetase (Hccs), mRNA mm
RG-U34B:RC_AA899828_AT similar to Hccs protein rn
RAE230A:1390408_AT similar to Hccs protein rn
RAT230_2:1390408_AT similar to Hccs protein rn
RAE230A:1377265_AT Similar to Hccs protein rn
RAT230_2:1377265_AT Similar to Hccs protein rn
YEAST_2:1777723_AT Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5739 mitochondrion inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4408 holocytochrome-c synthase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88969 Hypothetical protein T06D8.6 [Caenorhabditis elegans] ref|NP_496403.1| T06D8.6 [Caenorhabditis elegans] sp|P53703|CCHL_CAEEL Probable cytochrome C-type heme lyase (CCHL) (Holocytochrome-C-type synthase) 1.0E-169
blast CAE59548 Hypothetical protein CBG02944 [Caenorhabditis briggsae] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR000511 EMBL-EBI Cytochrome c and c1 heme-lyase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191544_S_AT
gccattcgcacttccaacgaaacgagagaagtctacaatcccgaaagctggaacagaaac
tgaaacctggacctatccaagtccccaaatgttctggaacgccatgctgaaaaaaggatg
gagatggcaagatgactcgctgagcaaaagcgacatggaaaacattatcagcatccataa
cgctaacaacgaggaggcatggagagaggtgttgaaatgggagaatcttcttcatccgga
atgcgcagagccgaaactgaaaagcttcaaaggagatgccaagaacctatcgccaagagc
tcgtttccgcaacttgttcctcggatacgatctcccattcgatcgtcacgattggattgt
tgatagatgcggaaccaagcaagttcagtacgtcattgactactacgatggtggtgctgt
ggatccaagctcaaagcttttcactattcttgatgttcgaccggccgtgaatgatattgg
aaacatctgggatcgaatggttgttgcttattggagattcaaattcgagacacttggatt
cacaccgagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCATTCGCACTTCCAACGAAACGA 689 279 264 Antisense
GAGAAGTCTACAATCCCGAAAGCTG 636 391 289 Antisense
CTGGACCTATCCAAGTCCCCAAATG 419 241 330 Antisense
AAGAACCTATCGCCAAGAGCTCGTT 508 151 544 Antisense
GTTTCCGCAACTTGTTCCTCGGATA 430 451 566 Antisense
TCTCCCATTCGATCGTCACGATTGG 300 611 594 Antisense
GACTACTACGATGGTGGTGCTGTGG 403 371 661 Antisense
GGTGCTGTGGATCCAAGCTCAAAGC 436 501 676 Antisense
TCACTATTCTTGATGTTCGACCGGC 212 619 704 Antisense
GTTCGACCGGCCGTGAATGATATTG 7 443 718 Antisense
CGAGACACTTGGATTCACACCGAGC 264 251 789 Antisense
Sequence Source Affymetrix Proprietary Database