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[191540_at] protein releasing factor

Description W03F8.3 /REP_DB=WormBase Gene ID /WP=CE17273 /TR=SW:O44568 /GB=AAB94190.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein releasing factor
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 91      6grp_total > 47      4grp_total > 54      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 231.5
(3)
P 		178.6
(-0.9)
P 		157.2
(-2.5)
P 		150.8
(-3)
P 		81
B 237.7
(3)
P 		174.4
(0.3)
P 		174
(0.3)
P 		98.3
(-3)
P 		139
C 283.1
(3)
P 		204
(-2.2)
P 		232.1
(-0.4)
P 		191.9
(-3)
P 		91
avr 250.8
(3) 		185.7
(-0.8) 		187.8
(-0.6) 		147
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 256.4
(3)
P 		174.7
(-3)
P 		212
(-0.3)
P 		186.3
(-2.1)
P 		202.6
(-1)
P 		244.8
(2.1)
P 		82
B 268.7
(3)
P 		232.8
(1)
P 		161.8
(-3)
P 		163
(-2.9)
P 		237.4
(1.2)
P 		202.5
(-0.7)
P 		107
C 136.3
(-3)
P 		251.9
(2.7)
P 		170.8
(-1.3)
P 		171.7
(-1.2)
P 		140.8
(-2.8)
P 		257.2
(3)
P 		121
avr 220.5
(1.6) 		219.8
(1.5) 		181.5
(-2.2) 		173.7
(-3) 		193.6
(-1) 		234.8
(3) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 84.3
(3)
P 		38.8
(-1.4)
P 		41.1
(-1.2)
P 		22.1
(-3)
P 		62
B 75.8
(3)
P 		43.1
(-0.6)
P 		40.3
(-0.9)
P 		21
(-3)
P 		55
C 93.7
(3)
P 		58.6
(-0.4)
P 		80.9
(1.7)
P 		32.4
(-3)
P 		61
avr 84.6
(3) 		46.8
(-0.8) 		54.1
(-0.1) 		25.2
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 47.3
(-2.6)
P 		62.1
(3)
P 		46.2
(-3)
P 		47.3
(-2.6)
P 		61.1
(2.6)
P 		59.6
(2.1)
P 		16
B 93.5
(2.7)
P 		77.5
(1)
P 		39.3
(-3)
P 		43.2
(-2.6)
P 		96.4
(3)
P 		75.5
(0.8)
P 		57
C 50.5
(-1.1)
P 		76.9
(1.9)
P 		43.5
(-2)
P 		34.5
(-3)
P 		45.6
(-1.7)
P 		86.1
(3)
P 		52
avr 63.8
(1.1) 		72.2
(2.7) 		43
(-2.8) 		41.7
(-3) 		67.7
(1.9) 		73.7
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191540_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10924
Sequence Type Exemplar sequence
Representative Public ID W03F8.3 NCBI
Target Description W03F8.3 /REP_DB=WormBase Gene ID /WP=CE17273 /TR=SW:O44568 /GB=AAB94190.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein releasing factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068336(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068336 NCBI Caenorhabditis elegans W03F8.3 (W03F8.3) mRNA, complete cds. 11/11 None
SNAP00000036704 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5187145:5188982:1 11/11 None
GENEFINDER00000036715 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5187145:5188982:1 11/11 None
W03F8.3 ENSEMBL cdna:known chromosome:CEL140:IV:5185323:5189021:1 gene:W03F8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5187150-5188988(+) 100.0 100.0
Public Domain and Genome References
Gene Title protein releasing factor
Ensembl W03F8.3
Entrez Gene 177294 Entrez gene
SwissProt O44568 EMBL-EBI
WormBase CE17273 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:266751_AT peptide chain release factor, putative at
CANINE:1604898_AT similar to mitochondrial translational release factor 1-like cfa
CANINE_2:CFA.19419.1.S1_S_AT similar to mitochondrial translational release factor 1-like cfa
DROSOPHILA_2:1637221_AT dm
DROSGENOME1:153925_AT dm
CHICKEN:GGA.15726.1.S1_AT mitochondrial translational release factor 1-like gga
HU35KSUBA:W87313_AT mitochondrial translational release factor 1-like hs
HU35KSUBD:RC_AA405016_S_AT mitochondrial translational release factor 1-like hs
HU35KSUBD:RC_AA620381_AT mitochondrial translational release factor 1-like hs
U133_X3P:HS.70488.0.A2_3P_A_AT mitochondrial translational release factor 1-like hs
HG-U133_PLUS_2:225215_S_AT mitochondrial translational release factor 1-like hs
HG-U133B:225215_S_AT mitochondrial translational release factor 1-like hs
HG-U95B:58699_AT mitochondrial translational release factor 1-like hs
HG-U95B:58701_G_AT mitochondrial translational release factor 1-like hs
HG-U95C:64910_AT mitochondrial translational release factor 1-like hs
HG-U133_PLUS_2:215560_X_AT Mitochondrial translational release factor 1-like hs
HG-U133A_2:215560_X_AT Mitochondrial translational release factor 1-like hs
HG-U133A:215560_X_AT Mitochondrial translational release factor 1-like hs
HG-U133_PLUS_2:217204_AT Mitochondrial translational release factor 1-like hs
HG-U133A_2:217204_AT Mitochondrial translational release factor 1-like hs
HG-U133A:217204_AT Mitochondrial translational release factor 1-like hs
HU35KSUBC:RC_AA463261_AT Similar to mitochondrial translational release factor 1-like hs
U133_X3P:HS.70488.0.A3_3P_A_AT Similar to mitochondrial translational release factor 1-like hs
U133_X3P:HS.274266.0.S1_3P_X_AT Mitochondrial translational release factor 1-like hs
U133_X3P:HS.274266.0.S1_3P_AT Mitochondrial translational release factor 1-like hs
U133_X3P:HS.225836.0.S1_3P_AT Mitochondrial translational release factor 1-like hs
U133_X3P:215560_3P_X_AT Mitochondrial translational release factor 1-like hs
HG-U133_PLUS_2:225206_S_AT Similar to mitochondrial translational release factor 1-like hs
HG-U133B:225206_S_AT Similar to mitochondrial translational release factor 1-like hs
HG-U95AV2:34123_AT Mitochondrial translational release factor 1-like hs
HG-U95B:58450_S_AT Similar to mitochondrial translational release factor 1-like hs
HG-U95AV2:34124_AT Similar to mitochondrial translational release factor 1-like hs
HG-U95C:49708_AT Mitochondrial translational release factor 1-like hs
HG-U95C:49711_G_AT Mitochondrial translational release factor 1-like hs
HG-U95C:51109_AT Mitochondrial translational release factor 1-like hs
HG-U95C:66006_S_AT Mitochondrial translational release factor 1-like hs
HU35KSUBB:RC_R44397_AT Similar to mitochondrial translational release factor 1-like hs
MOE430B:1438025_AT mitochondrial translational release factor 1-like mm
MOUSE430_2:1438025_AT mitochondrial translational release factor 1-like mm
MG-U74CV2:140050_AT Mitochondrial translational release factor 1-like (Mtrf1l), mRNA mm
MG-U74AV2:98388_AT Mitochondrial translational release factor 1-like (Mtrf1l), mRNA mm
MU11KSUBB:MSA.33489.0_S_AT Mitochondrial translational release factor 1-like (Mtrf1l), mRNA mm
MU19KSUBA:TC16984_AT Mitochondrial translational release factor 1-like (Mtrf1l), mRNA mm
RG-U34A:RC_AI639148_AT similar to mitochondrial translational release factor 1-like rn
RAE230A:1392654_AT similar to mitochondrial translational release factor 1-like rn
RAT230_2:1392654_AT similar to mitochondrial translational release factor 1-like rn
RAE230A:1375260_AT Similar to mitochondrial translational release factor 1-like rn
RAT230_2:1375260_AT Similar to mitochondrial translational release factor 1-like rn
YEAST_2:1770466_AT Mitochondrial polypeptide chain release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6310 DNA recombination inferred from electronic annotation QuickGO AmiGO
6415 translational termination inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
150 recombinase activity inferred from electronic annotation QuickGO AmiGO
3747 translation release factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB94190 Hypothetical protein W03F8.3 [Caenorhabditis elegans] ref|NP_500737.1| W03F8.3 [Caenorhabditis elegans] sp|O44568|RF1M_CAEEL Probable peptide chain release factor 1, mitochondrial precursor (MRF-1) 0.0
blast CAE72827 Hypothetical protein CBG20109 [Caenorhabditis briggsae] 1.0E-168
blast XP_614102 PREDICTED: similar to mitochondrial translational release factor 1-like isoform 2 [Bos taurus] 4.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR005139 EMBL-EBI PCRF domain 2.5E-15
Pfam IPR000352 EMBL-EBI Class I peptide chain release factor domain 3.6E-57
Sequence
Target Sequence 
>C. ELEGANS:191540_AT
tggaggagttcgaagtgctctcatcgcagtgtctggggagaaagtctacgcaaaaatgcg
cttcgaagcaggcgttcatcgagttcagcgagttcctgtcaacgattctcgaatgcatac
aagcacggcgagtatctcggtgctccccgagccagaagaggtttctgtggtggttccaag
tgattcggtgaagattgaagcaatgcgagcgtctggacctggcggtcagaatgtgaataa
gcgatcgacagccgtacggatgacacataaagagacgggaattgctgtgcattgtatgga
tgagagatttcaacatttaaatattcagattgcctataaacgtctcgcagccattttgat
gcaaaaacaagttgatgccatgctggaaaagatcgtttcaaaacgaaaacttcaagttgg
atccaaagctcgcgctgaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGGAGTTCGAAGTGCTCTCATC 501 549 561 Antisense
TGCTCTCATCGCAGTGTCTGGGGAG 456 581 576 Antisense
CGAAGCAGGCGTTCATCGAGTTCAG 625 251 624 Antisense
GAGTTCAGCGAGTTCCTGTCAACGA 226 399 641 Antisense
AGCACGGCGAGTATCTCGGTGCTCC 327 75 682 Antisense
GAAGAGGTTTCTGTGGTGGTTCCAA 177 339 715 Antisense
AGCGTCTGGACCTGGCGGTCAGAAT 305 1 768 Antisense
ATAAGCGATCGACAGCCGTACGGAT 54 25 797 Antisense
GATTGCCTATAAACGTCTCGCAGCC 15 435 888 Antisense
ACGTCTCGCAGCCATTTTGATGCAA 240 95 900 Antisense
TTGGATCCAAAGCTCGCGCTGAAAA 158 709 977 Antisense
Sequence Source Affymetrix Proprietary Database