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[191539_at] trehalose phosphate synthase

Description F19H8.1 /REP_DB=WormBase Gene ID /WP=CE15870 /TR=O45380 /GB=CAB07584.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=trehalose phosphate synthase
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 86      4grp_highest_8_8 > 30      4grp_high_8_8 > 04      6grp_highest_nonorm > 89      6grp_total > 76      4grp_total > 04      6grp_highest_sub > In      6grp_cgc > 48      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1561
1 2 3 4 range
A 2284.1
(-1.1)
P 		1846.5
(-3)
P 		1838.3
(-3)
P 		3258.7
(3)
P 		1420
B 2314.5
(-1.2)
P 		1884.3
(-3)
P 		1951.8
(-2.7)
P 		3285.9
(3)
P 		1402
C 2173.6
(-1.1)
P 		1725
(-3)
P 		1978.8
(-1.9)
P 		3156.8
(3)
P 		1432
avr 2257.4
(-1.1) 		1818.6
(-3) 		1923
(-2.6) 		3233.8
(3) 		1415

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1796
1 2 3 4 5 6 range
A 985.7
(-3)
P 		1127.9
(-2.5)
P 		1039.3
(-2.8)
P 		1404.9
(-1.5)
P 		1338.9
(-1.7)
P 		2657.4
(3)
P 		1672
B 939.4
(-2.7)
P 		861.3
(-3)
P 		1297.2
(-1.3)
P 		1455.9
(-0.7)
P 		1240.3
(-1.5)
P 		2388.4
(3)
P 		1527
C 956.5
(-2.7)
P 		891.9
(-3)
P 		1510.4
(-0.6)
P 		1252.9
(-1.6)
P 		1343.7
(-1.2)
P 		2436.2
(3)
P 		1544
avr 960.5
(-3) 		960.4
(-3) 		1282.3
(-1.7) 		1371.2
(-1.4) 		1307.6
(-1.6) 		2494
(3) 		1534
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 431
1 2 3 4 range
A 831.6
(3)
P 		400.8
(-3)
P 		480.6
(-1.9)
P 		477
(-1.9)
P 		431
B 738.1
(3)
P 		465.4
(-2.7)
P 		452.3
(-3)
P 		700.2
(2.2)
P 		286
C 719.5
(3)
P 		495.9
(-3)
P 		689.7
(2.2)
P 		532.3
(-2)
P 		224
avr 763.1
(3) 		454
(-3) 		540.9
(-1.3) 		569.8
(-0.8) 		309

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 709
1 2 3 4 5 6 range
A 181.7
(-3)
P 		401.1
(-0.2)
P 		226.3
(-2.4)
P 		356.3
(-0.7)
P 		404
(-0.1)
P 		647
(3)
P 		465
B 326.9
(-2.6)
P 		286.7
(-3)
P 		315
(-2.7)
P 		385.6
(-2)
P 		503.9
(-0.8)
P 		890.6
(3)
P 		604
C 354.5
(-1.9)
P 		272.2
(-2.8)
P 		384.4
(-1.6)
P 		252
(-3)
P 		434.8
(-1.1)
P 		815.5
(3)
P 		564
avr 287.7
(-3) 		320
(-2.6) 		308.6
(-2.7) 		331.3
(-2.5) 		447.6
(-1.1) 		784.4
(3) 		497
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191539_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3632
Sequence Type Exemplar sequence
Representative Public ID F19H8.1 NCBI
Target Description F19H8.1 /REP_DB=WormBase Gene ID /WP=CE15870 /TR=O45380 /GB=CAB07584.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=trehalose phosphate synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064634(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064634 NCBI Caenorhabditis elegans Trehalose 6-Phosphate Synthase family member (tps-2) (tps-2) mRNA, complete cds. 11/11 None
SNAP00000003959 ENSEMBL cdna:SNAP chromosome:CEL140:II:14607103:14610303:1 11/11 None
GENEFINDER00000003970 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14607103:14610303:1 11/11 None
F19H8.1.2 ENSEMBL cdna:known chromosome:CEL140:II:14600787:14610442:1 gene:F19H8.1 11/11 None
F19H8.1.1 ENSEMBL cdna:known chromosome:CEL140:II:14600793:14610442:1 gene:F19H8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14600643-14610300(+) 100.0 100.0
Public Domain and Genome References
Gene Title trehalose phosphate synthase
Gene Symbol tps-2
Ensembl F19H8.1
Entrez Gene 3565050 Entrez gene
SwissProt O45380 EMBL-EBI
WormBase CE37507 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5992 trehalose biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3825 alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07584 Hypothetical protein F19H8.1 [Caenorhabditis elegans] emb|CAH18872.1| putative trehalose 6-phosphate synthase [Caenorhabditis elegans] ref|NP_497035.3| Trehalose 6-Phosphate Synthase family member (tps-2) [Caenorhabditis elegans] 0.0
blast CAD54507 trehalose-6-phosphate synthase [Caenorhabditis elegans] 0.0
blast CAE65906 Hypothetical protein CBG11073 [Caenorhabditis briggsae] 0.0
blast CAB07584 Hypothetical protein F19H8.1 [Caenorhabditis elegans] emb|CAH18872.1| putative trehalose 6-phosphate synthase [Caenorhabditis elegans] ref|NP_497035.3| Trehalose 6-Phosphate Synthase family member (tps-2) [Caenorhabditis elegans] 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR006689 EMBL-EBI ARF/SAR superfamily 3.3E-91
Pfam IPR006689 EMBL-EBI ARF/SAR superfamily 1.4E-75
Pfam IPR001830 EMBL-EBI Glycosyl transferase, family 20 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191539_AT
aaggctgatgtcctagcaagcgcgtacacacgaatctctcacttgctagaggagcccgag
ttccgtccattcacatgggttggctcaggactacaaaagcattacggtcacttgaccatt
gcgttccaagacgtctacaagacaatcaccgaggctcaaggaaaacagctacacgaagaa
attgagaaaattgtgaaggatgtggatccacatggtacacgtcttcaattggctagcact
gaatttgacattaaggtttatatgaagacagaaacagatggtcacgtattcgataaggga
gatggattaagattgctgtgtgagaaaatgcactgtgatttgacggaaggcaacgtgttg
gtatgcggagactcttccactgatattcctatgcttaaggaatgtctgattagaaatcca
aaaggagtctacacaatttgggttactgtcaatgataaattgaaggaagaggtccgcgct
ctctgcgcatcctactcaaactccaacgtcgcatttgtctcgtgcccagaggttctgctt
ggtgccatggcccaagccaccatccgagagataacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGCTGATGTCCTAGCAAGCGCGT 494 161 3184 Antisense
GCAAGCGCGTACACACGAATCTCTC 355 321 3199 Antisense
ACTTGCTAGAGGAGCCCGAGTTCCG 481 95 3224 Antisense
GTTCCGTCCATTCACATGGGTTGGC 176 441 3243 Antisense
GACCATTGCGTTCCAAGACGTCTAC 205 381 3297 Antisense
GTCTACAAGACAATCACCGAGGCTC 74 469 3316 Antisense
ATCCACATGGTACACGTCTTCAATT 343 39 3389 Antisense
GGAGACTCTTCCACTGATATTCCTA 150 519 3550 Antisense
CAAACTCCAACGTCGCATTTGTCTC 694 191 3680 Antisense
TGCCCAGAGGTTCTGCTTGGTGCCA 332 585 3706 Antisense
CCAAGCCACCATCCGAGAGATAACC 368 277 3735 Antisense
Sequence Source Affymetrix Proprietary Database