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[191530_at] Putative membrane protein

Description ZK849.5 /REP_DB=WormBase Gene ID /WP=CE16750 /TR=SW:O18304 /GB=CAB05028.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster 6grp_total > 86      4grp_total > 42      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 6.2
(-3)
A 		19.4
(3)
A 		14.5
(0.8)
A 		17.3
(2)
A 		13
B 9.4
(-2.1)
A 		24
(3)
A 		16.2
(0.3)
A 		6.9
(-3)
A 		17
C 8.7
(-3)
A 		26
(3)
A 		10.2
(-2.5)
A 		17
(-0.1)
A 		17
avr 8.1
(-3) 		23.1
(3) 		13.6
(-0.8) 		13.7
(-0.8) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 9.4
(-2)
A 		7.3
(-3)
A 		7.8
(-2.8)
A 		17
(1.6)
A 		18.8
(2.4)
A 		20
(3)
A 		13
B 11.2
(-1.6)
A 		13.5
(-0.5)
A 		20.8
(3)
A 		8.2
(-3)
A 		14.4
(-0)
A 		15.3
(0.4)
A 		13
C 8.5
(-3)
A 		13.4
(-0.4)
A 		9.2
(-2.6)
A 		16.2
(1.1)
A 		9.3
(-2.6)
A 		19.8
(3)
A 		11
avr 9.7
(-3) 		11.4
(-1.8) 		12.6
(-1) 		13.8
(-0.2) 		14.2
(0.1) 		18.4
(3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 2.2
(-3)
A 		4.2
(3)
A 		3.8
(1.8)
A 		2.5
(-2.1)
A 		2
B 3
(-1)
A 		5.9
(3)
A 		3.8
(0.1)
A 		1.5
(-3)
A 		4
C 2.9
(-3)
A 		7.5
(3)
A 		3.5
(-2.2)
A 		2.9
(-3)
A 		5
avr 2.7
(-2.3) 		5.9
(3) 		3.7
(-0.7) 		2.3
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 1.7
(-3)
A 		2.6
(-1.7)
A 		1.7
(-3)
A 		4.3
(0.9)
A 		5.7
(3)
A 		4.9
(1.8)
A 		4
B 3.9
(-0.2)
A 		4.5
(0.7)
A 		5.1
(1.7)
A 		2.2
(-3)
A 		5.9
(3)
A 		5.7
(2.7)
A 		4
C 3.1
(-1.9)
A 		4.1
(-0.5)
A 		2.3
(-3)
A 		3.3
(-1.6)
A 		3
(-2)
A 		6.6
(3)
A 		4
avr 2.9
(-3) 		3.7
(-1.3) 		3
(-2.8) 		3.3
(-2.1) 		4.9
(1.3) 		5.7
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191530_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1813
Sequence Type Exemplar sequence
Representative Public ID ZK849.5 NCBI
Target Description ZK849.5 /REP_DB=WormBase Gene ID /WP=CE16750 /TR=SW:O18304 /GB=CAB05028.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061077(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061077 NCBI Caenorhabditis elegans ZK849.5 (ZK849.5) mRNA, complete cds. 11/11 None
GENEFINDER00000021698 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14210182:14212761:1 11/11 None
ZK849.5 ENSEMBL cdna:known chromosome:CEL140:I:14208138:14212761:1 gene:ZK849.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021691 4/11 Cross Hyb Matching Probes None
SNAP00000021692 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14208269-14212692(+) 100.0 100.0
Public Domain and Genome References
Gene Title Putative membrane protein
Ensembl ZK849.5
Entrez Gene 173291 Entrez gene
SwissProt O18304 EMBL-EBI
WormBase CE16750 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05028 Hypothetical protein ZK849.5 [Caenorhabditis elegans] ref|NP_493478.1| ZK849.5 [Caenorhabditis elegans] sp|O18304|YHS5_CAEEL Hypothetical protein ZK849.5 in chromosome I 0.0
blast CAB05028 Hypothetical protein ZK849.5 [Caenorhabditis elegans] ref|NP_493478.1| ZK849.5 [Caenorhabditis elegans] sp|O18304|YHS5_CAEEL Hypothetical protein ZK849.5 in chromosome I 1.0E-179
blast CAB05710 Hypothetical protein C49A1.2 [Caenorhabditis elegans] emb|CAB05029.1| Hypothetical protein C49A1.2 [Caenorhabditis elegans] ref|NP_493480.1| C49A1.2 [Caenorhabditis elegans] 2.0E-93
blast CAB05027 Hypothetical protein ZK849.4 [Caenorhabditis elegans] ref|NP_493479.1| ZK849.4 [Caenorhabditis elegans] sp|O18303|YHS4_CAEEL Hypothetical protein ZK849.4 in chromosome I 4.0E-72
blast CAB05027 Hypothetical protein ZK849.4 [Caenorhabditis elegans] ref|NP_493479.1| ZK849.4 [Caenorhabditis elegans] sp|O18303|YHS4_CAEEL Hypothetical protein ZK849.4 in chromosome I 5.0E-72
blast CAB05710 Hypothetical protein C49A1.2 [Caenorhabditis elegans] emb|CAB05029.1| Hypothetical protein C49A1.2 [Caenorhabditis elegans] ref|NP_493480.1| C49A1.2 [Caenorhabditis elegans] 5.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493478.1 3 29-51,235-257,270-292
ZK849.5 3 29-51,235-257,270-292
GENEFINDER00000021698 2 174-196,209-231
Sequence
Target Sequence 
>C. ELEGANS:191530_AT
ggcgaacgagttcacaatcctgtggggtcaaatcaaggagtggcagacaaagctctcact
tttgcggaactgcgactacatcccgattccgttggcgtacccgcaggctgtgttcctagc
cgttcgttgttactttgcagtgtgtgtgttcacaagacagcacttggatagatatgattc
gaagatgcacacctggataactttcttcccagtgctcacaacattccaatacattttcat
gatgggctggatgaaggtcgccgaaatcctgctcaacccaatgggagaagacgaagacga
ttttgagctgaactttatcattgataacaatcttaaaaatggattggatattgtgagtgg
attatgtggaaaccataggaaacttgcagaacatgaaattgaaaatgattgtcgtccata
ttatcaaaccaatgaacaagatcgtaaaaagaatcgtgcaccgccggaatccttgaagaa
cgtggagttcaaatcattcacaatggaaaaggcttctaaggactcgccaatggttgtgaa
atcggaatctgtttgttgcctacgccggagattcaatag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCGAACGAGTTCACAATCCTGTGG 517 539 636 Antisense
GGCAGACAAAGCTCTCACTTTTGCG 47 537 677 Antisense
TTTTGCGGAACTGCGACTACATCCC 270 677 695 Antisense
TCCTAGCCGTTCGTTGTTACTTTGC 542 591 749 Antisense
GCACACCTGGATAACTTTCTTCCCA 389 317 822 Antisense
TTCCCAGTGCTCACAACATTCCAAT 601 697 841 Antisense
GATTGTCGTCCATATTATCAAACCA 293 435 1042 Antisense
AAAAAGAATCGTGCACCGCCGGAAT 662 129 1081 Antisense
GCTTCTAAGGACTCGCCAATGGTTG 556 303 1147 Antisense
AATCGGAATCTGTTTGTTGCCTACG 442 169 1175 Antisense
GTTGCCTACGCCGGAGATTCAATAG 319 437 1190 Antisense
Sequence Source Affymetrix Proprietary Database