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[191525_at] olfacory channel

Description B0212.5 /REP_DB=WormBase Gene ID /WP=CE20445 /GEN=osm-9 /TR=O17469 /GB=AAC02569.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=olfacory channel
Cluster 6grp_total > 70      4grp_total > 04      6grp_cgc > 95      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 19.6
(-1.8)
A 		8.4
(-3)
A 		13.4
(-2.5)
A 		64.4
(3)
A 		56
B 13.5
(3)
A 		12.3
(2)
A 		7.2
(-2.3)
A 		6.3
(-3)
A 		7
C 6.7
(-3)
A 		11
(3)
A 		8.4
(-0.6)
A 		7.5
(-1.9)
A 		4
avr 13.3
(-1.7) 		10.6
(-2.7) 		9.7
(-3) 		26.1
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 28.2
(2)
A 		5.9
(-3)
A 		27.8
(2)
A 		7.8
(-2.6)
A 		9.9
(-2.1)
A 		32.4
(3)
A 		27
B 6.4
(-0.8)
A 		9.4
(1.4)
A 		7.7
(0.2)
A 		7.3
(-0.1)
A 		11.5
(3)
A 		3.4
(-3)
A 		8
C 5.4
(-3)
A 		5.3
(-3)
A 		9.2
(-2.7)
A 		85
(3)
A 		9.4
(-2.7)
A 		20.9
(-1.8)
A 		80
avr 13.3
(-1.6) 		6.9
(-3) 		14.9
(-1.2) 		33.4
(3) 		10.3
(-2.2) 		18.9
(-0.3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 7.1
(1.2)
A 		1.8
(-3)
A 		3.5
(-1.7)
A 		9.4
(3)
A 		8
B 4.3
(3)
A 		3
(0.4)
A 		1.7
(-2.2)
A 		1.3
(-3)
A 		3
C 2.2
(-0.2)
A 		3.2
(3)
A 		2.9
(2.1)
A 		1.3
(-3)
A 		2
avr 4.5
(3) 		2.7
(-3) 		2.7
(-3) 		4
(1.3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 5.2
(0.3)
A 		2.1
(-2.9)
A 		6.1
(1.2)
A 		2
(-3)
A 		3
(-2)
A 		7.9
(3)
A 		6
B 2.2
(-1.4)
A 		3.1
(0.2)
A 		1.9
(-1.9)
A 		1.9
(-1.9)
A 		4.7
(3)
A 		1.3
(-3)
A 		3
C 2
(-2.8)
A 		1.6
(-3)
A 		2.3
(-2.7)
A 		17.1
(3)
A 		3.1
(-2.4)
A 		7
(-0.9)
A 		16
avr 3.1
(-2) 		2.3
(-3) 		3.4
(-1.6) 		7
(3) 		3.6
(-1.3) 		5.4
(1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191525_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10494
Sequence Type Exemplar sequence
Representative Public ID B0212.5 NCBI
Target Description B0212.5 /REP_DB=WormBase Gene ID /WP=CE20445 /GEN=osm-9 /TR=O17469 /GB=AAC02569.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=olfacory channel
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067971(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067971 NCBI Caenorhabditis elegans OSMotic avoidance abnormal family member (osm-9) (osm-9) mRNA, complete cds. 11/11 None
SNAP00000040979 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3551147:3556677:-1 11/11 None
GENEFINDER00000040987 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3551147:3557427:-1 11/11 None
B0212.5 ENSEMBL cdna:known chromosome:CEL140:IV:3551086:3557427:-1 gene:B0212.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3551152-3557433(-) 100.0 100.0
Public Domain and Genome References
Gene Title olfacory channel
Gene Symbol osm-9
Ensembl B0212.5
Entrez Gene 177117 Entrez gene
SwissProt O17469 EMBL-EBI
WormBase CE20445 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1625881_AT dm
DROSGENOME1:144617_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC02569 Osmotic avoidance abnormal protein 9 [Caenorhabditis elegans] ref|NP_500372.1| OSMotic avoidance abnormal family member (osm-9) [Caenorhabditis elegans] gb|AAB87064.1| olfactory channel [Caenorhabditis elegans] 0.0
blast CAE69021 Hypothetical protein CBG15022 [Caenorhabditis briggsae] 0.0
blast EAL32637 GA18240-PA [Drosophila pseudoobscura] 1.0E-164
Protein Domains
Database ID Description E-Value
Pfam IPR002110 EMBL-EBI Ankyrin 3.4E-7
Pfam IPR002110 EMBL-EBI Ankyrin 5.4E-8
Pfam IPR002110 EMBL-EBI Ankyrin 0.72
Pfam IPR002110 EMBL-EBI Ankyrin 0.18
Pfam IPR002110 EMBL-EBI Ankyrin 3.4E-7
Pfam IPR002110 EMBL-EBI Ankyrin 5.4E-8
Pfam IPR002110 EMBL-EBI Ankyrin 0.72
Pfam IPR002110 EMBL-EBI Ankyrin 0.18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500372.1 7 395-414,429-448,468-490,500-519,540-558,578-600,621-643
B0212.5 7 395-414,429-448,468-490,500-519,540-558,578-600,621-643
SNAP00000040979 7 356-375,390-409,429-451,461-480,501-519,539-561,582-604
GENEFINDER00000040987 7 395-414,429-448,468-490,500-519,540-558,578-600,621-643
Sequence
Target Sequence 
>C. ELEGANS:191525_AT
cgaacaagcagtactccttcttcatggccacgatcgcctcgacagagtctacgaagatca
tgttcagcctgagaaagttcccagtcgttcgagaactccaacacgtatcggaactacact
gaattcttcaaaacgacttaagactactacaatggttgttggcgccgctgtgaccaacac
tcatgaggtacgaacggatgaagctgtgaatagcatgctcctgtcagctccgccgagtct
atcaggtgaaggtgcaacgatggactggcagccgtctattacaccggttgaggaacggag
tgaatcgaaatcacaggaggatcggtcagaagcttccacaccaaatctgggaattcatag
gacgaccccaaaagctgattcgccgattagagttgtggaatatagccgcacaatccgagt
ccgcgcggccgacactatcccgtccatcgaacttccgaacatcccgacgaagcagacgtc
ttcaacgcccccacatcgtgcagtatcaccacgtctccgtgcggatatgttcaggagaca
ccagcaaccggcttcgtttgatcagagtcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAACAAGCAGTACTCCTTCTTCAT 1 253 2223 Antisense
CGATCGCCTCGACAGAGTCTACGAA 286 249 2253 Antisense
GCCTGAGAAAGTTCCCAGTCGTTCG 217 283 2289 Antisense
CTCCGCCGAGTCTATCAGGTGAAGG 203 235 2450 Antisense
TGCAACGATGGACTGGCAGCCGTCT 288 577 2475 Antisense
GCAGCCGTCTATTACACCGGTTGAG 134 313 2490 Antisense
GAGGATCGGTCAGAAGCTTCCACAC 253 401 2539 Antisense
GAATATAGCCGCACAATCCGAGTCC 707 325 2620 Antisense
TCCGAACATCCCGACGAAGCAGACG 97 595 2676 Antisense
TCACCACGTCTCCGTGCGGATATGT 26 625 2728 Antisense
AACCGGCTTCGTTTGATCAGAGTCC 494 145 2768 Antisense
Sequence Source Affymetrix Proprietary Database