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[191520_s_at] intiation factor/helicase

Description F33D11.10 /REP_DB=WormBase Gene ID /WP=CE09901 /TR=O44781 /GB=AAB96704.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=intiation factor-helicase
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 67      6grp_total > 56      4grp_total > 03      6grp_high1_sub > Cu      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1311
1 2 3 4 range
A 1808
(-0.5)
P 		1518.1
(-2.8)
P 		1496.6
(-3)
P 		2243
(3)
P 		746
B 1376.9
(-3)
P 		1454.7
(-2.6)
P 		1485.3
(-2.5)
P 		2688.3
(3)
P 		1311
C 1911.8
(-0.4)
P 		1707.6
(-3)
P 		1920.1
(-0.3)
P 		2175.6
(3)
P 		468
avr 1698.9
(-2) 		1560.1
(-3) 		1634
(-2.5) 		2369
(3) 		809

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1178
1 2 3 4 5 6 range
A 2046.9
(3)
P 		1481.3
(-3)
P 		1652.1
(-1.2)
P 		1717.7
(-0.5)
P 		1573.4
(-2)
P 		1892
(1.4)
P 		566
B 2038.4
(0.6)
P 		2212
(1.7)
P 		2218.8
(1.8)
P 		1720.3
(-1.6)
P 		1506.6
(-3)
P 		2403.2
(3)
P 		897
C 1546.8
(-1.9)
P 		2149.5
(1.2)
P 		1359.8
(-2.8)
P 		1705.7
(-1.1)
P 		1326.9
(-3)
P 		2504.4
(3)
P 		1178
avr 1877.4
(0.1) 		1947.6
(0.6) 		1743.6
(-0.9) 		1714.6
(-1.2) 		1469
(-3) 		2266.5
(3) 		798
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 341
1 2 3 4 range
A 658.3
(3)
P 		329.5
(-3)
P 		391.3
(-1.9)
P 		328.3
(-3)
P 		330
B 439.1
(-0.5)
P 		359.3
(-2.6)
P 		344.2
(-3)
P 		572.9
(3)
P 		229
C 632.8
(2.3)
P 		490.9
(-0.5)
P 		669.2
(3)
P 		366.8
(-3)
P 		302
avr 576.7
(3) 		393.2
(-3) 		468.2
(-0.5) 		422.7
(-2) 		184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 553
1 2 3 4 5 6 range
A 377.3
(-2.4)
P 		526.8
(3)
P 		359.8
(-3)
P 		435.6
(-0.3)
P 		474.8
(1.1)
P 		460.6
(0.6)
P 		167
B 709.3
(0.5)
P 		736.3
(0.8)
P 		538.8
(-1.9)
P 		455.7
(-3)
P 		612.1
(-0.9)
P 		896.1
(3)
P 		440
C 573.3
(-0.2)
P 		655.9
(0.8)
P 		346.1
(-3)
P 		343
(-3)
P 		429.4
(-2)
P 		838.4
(3)
P 		495
avr 553.3
(-0.3) 		639.7
(1.3) 		414.9
(-2.9) 		411.4
(-3) 		505.4
(-1.2) 		731.7
(3) 		320
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191520_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1581
Sequence Type Exemplar sequence
Representative Public ID F33D11.10 NCBI
Target Description F33D11.10 /REP_DB=WormBase Gene ID /WP=CE09901 /TR=O44781 /GB=AAB96704.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=intiation factor-helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059302(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059302 NCBI Caenorhabditis elegans F33D11.10 (F33D11.10) mRNA, complete cds. 11/11 None
SNAP00000028960 ENSEMBL cdna:SNAP chromosome:CEL140:I:5874899:5876265:-1 11/11 None
GENEFINDER00000028969 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5874899:5876265:-1 11/11 None
F33D11.10.2 ENSEMBL cdna:known chromosome:CEL140:I:5874559:5876279:-1 gene:F33D11.10 11/11 None
F33D11.10.1 ENSEMBL cdna:known chromosome:CEL140:I:5874710:5876275:-1 gene:F33D11.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5874898-5876265(-) 100.0 100.0
Public Domain and Genome References
Gene Title intiation factor/helicase
Ensembl F33D11.10
Entrez Gene 172258 Entrez gene
SwissProt O44781 EMBL-EBI
WormBase CE09901 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17470_AT eukaryotic translation initiation factor 4A, putative / eIF-4A, putative / DEAD box RNA helicase, putative at
ATH1-121501:257023_AT eukaryotic translation initiation factor 4A, putative / eIF-4A, putative / DEAD box RNA helicase, putative at
CANINE:1603683_S_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 cfa
CANINE:1599587_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 cfa
CANINE:1583525_S_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 cfa
CANINE:1583524_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 cfa
CANINE_2:CFA.12179.1.A1_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 cfa
CANINE_2:CFAAFFX.9378.1.S1_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 cfa
DROSOPHILA_2:1641498_AT dm
DROSGENOME1:149487_AT dm
CHICKEN:GGA.7844.1.S1_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 gga
HG-U133_PLUS_2:201303_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 hs
HG-U133A:201303_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 hs
HG-U133A_2:201303_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 hs
HUGENEFL:D21853_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 hs
U133_X3P:G7661919_3P_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 hs
HG-U95AV2:38031_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 hs
MU11KSUBB:MSA.30713.0_F_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MU11KSUBB:MSA.9866.0_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MU11KSUBB:MSA.10669.0_F_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MG-U74AV2:101485_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MG-U74BV2:164744_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MG-U74CV2:170484_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MOE430A:1417242_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MOUSE430A_2:1417242_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MOUSE430_2:1417242_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 mm
MOE430B:1436700_A_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 (Ddx48), mRNA mm
MOUSE430_2:1436700_A_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 (Ddx48), mRNA mm
MU19KSUBB:TC34245_F_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 (Ddx48), mRNA mm
MU19KSUBB:TC34245_I_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 (Ddx48), mRNA mm
YEAST_2:1776348_AT Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB96704 Hypothetical protein F33D11.10 [Caenorhabditis elegans] ref|NP_491703.1| F33D11.10 [Caenorhabditis elegans] 0.0
blast AAK29954 Hypothetical protein Y65B4A.6 [Caenorhabditis elegans] ref|NP_490761.2| Y65B4A.6 [Caenorhabditis elegans] 0.0
blast CAE61310 Hypothetical protein CBG05145 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.8E-37
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 3.7E-78
Sequence
Target Sequence 
>C. ELEGANS:191520_S_AT
gatatcttccacctggagcacaagtcgtacttctgtcagccacactgcctcatgaaattc
tagagatgaccagtaaattcatgacagatcctatcagaattcttgtgaaacgtgatgaac
tgacacttgaaggaatcaagcaattcttcgttgcagttgacagagaagaatggaagttcg
acactcttattgatctttatgatactcttaccatcacacaggctgtactattctgtaata
cccgtagaaaggttgattggttgacggataaaatgaaggaagccaacttcacagtgtcat
caatgcacggagatatggaacagaaggatcgtgatgaagttatgaaagagttcagagcag
gaacaacgagagtgctcatttcaactgatgtttgggcgagaggattagatgttccacaag
tctcgttggttatcaactatgatcttccaaacaatcgtgaattatacattcatcgtattg
gtcgttctggacgtttcggacgcaagggagtcgccatcaactttgtgaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATCTTCCACCTGGAGCACAAGT 99 427 593 Antisense
AGCACAAGTCGTACTTCTGTCAGCC 328 75 609 Antisense
GCCACACTGCCTCATGAAATTCTAG 209 279 631 Antisense
AGTTCGACACTCTTATTGATCTTTA 233 65 767 Antisense
ATACTCTTACCATCACACAGGCTGT 42 25 794 Antisense
GGCTGTACTATTCTGTAATACCCGT 503 509 813 Antisense
GGAAGCCAACTTCACAGTGTCATCA 270 531 870 Antisense
GAGAGTGCTCATTTCAACTGATGTT 620 395 960 Antisense
GATGTTCCACAAGTCTCGTTGGTTA 627 409 1000 Antisense
ATTCATCGTATTGGTCGTTCTGGAC 675 9 1060 Antisense
GGAGTCGCCATCAACTTTGTGAAGC 445 517 1099 Antisense
Sequence Source Affymetrix Proprietary Database