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[191517_at] 7TM chemoreceptor, srh family

Description T22F3.6 /REP_DB=WormBase Gene ID /WP=CE13972 /GEN=srh-212 /TR=Q94303 /GB=AAB18309.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 21      4grp_total > 31      6grp_cgc > 62      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 74.5
(3)
A 		7.9
(-2.9)
A 		6.8
(-3)
A 		59.4
(1.7)
A 		68
B 60.3
(3)
A 		12.3
(-2.6)
A 		9.3
(-3)
A 		15.6
(-2.3)
A 		51
C 92.8
(3)
A 		20.4
(-2.2)
A 		9.2
(-3)
A 		50.3
(-0.1)
A 		84
avr 75.9
(3) 		13.5
(-2.5) 		8.4
(-3) 		41.8
(-0) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 15
(-1.6)
A 		56.7
(3)
A 		34.4
(0.6)
A 		36.6
(0.8)
A 		1.9
(-3)
A 		6.9
(-2.5)
A 		55
B 64.6
(3)
A 		49.2
(1.4)
A 		9.2
(-2.8)
A 		11.6
(-2.6)
A 		7.5
(-3)
A 		7.7
(-3)
A 		57
C 8.8
(-2.6)
A 		61.6
(1.9)
A 		75
(3)
A 		27.1
(-1.1)
A 		7.1
(-2.8)
A 		4.4
(-3)
A 		71
avr 29.5
(-0.1) 		55.8
(3) 		39.5
(1.1) 		25.1
(-0.7) 		5.5
(-3) 		6.3
(-2.9) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 27.1
(3)
A 		1.7
(-3)
A 		1.8
(-3)
A 		8.7
(-1.3)
A 		25
B 19.2
(3)
A 		3
(-2.7)
A 		2.2
(-3)
A 		3.3
(-2.6)
A 		17
C 30.7
(3)
A 		5.9
(-2.4)
A 		3.2
(-3)
A 		8.5
(-1.8)
A 		28
avr 25.7
(3) 		3.5
(-2.7) 		2.4
(-3) 		6.8
(-1.9) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 2.8
(-2.3)
A 		20.2
(3)
A 		7.5
(-0.9)
A 		9.3
(-0.3)
A 		0.6
(-3)
A 		1.7
(-2.7)
A 		20
B 22.5
(3)
A 		16.4
(1.2)
A 		2.2
(-3)
A 		3.1
(-2.7)
A 		3.1
(-2.7)
A 		2.9
(-2.8)
A 		20
C 3.3
(-2.4)
A 		18.8
(2.9)
A 		19.1
(3)
A 		5.5
(-1.6)
A 		2.3
(-2.7)
A 		1.5
(-3)
A 		18
avr 9.5
(-0.3) 		18.5
(3) 		9.6
(-0.2) 		6
(-1.5) 		2
(-3) 		2
(-3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191517_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15294
Sequence Type Exemplar sequence
Representative Public ID T22F3.6 NCBI
Target Description T22F3.6 /REP_DB=WormBase Gene ID /WP=CE13972 /GEN=srh-212 /TR=Q94303 /GB=AAB18309.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071610(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071610 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-212) (srh-212) mRNA, complete cds. 11/11 None
SNAP00000034228 ENSEMBL cdna:SNAP chromosome:CEL140:V:3595006:3596337:-1 11/11 None
GENEFINDER00000034236 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3595006:3600407:-1 11/11 None
T22F3.6 ENSEMBL cdna:known chromosome:CEL140:V:3595006:3596960:-1 gene:T22F3.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3595005-3596960(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-212
Ensembl T22F3.6
Entrez Gene 188750 Entrez gene
SwissProt Q94303 EMBL-EBI
WormBase CE13972 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB18310 Hypothetical protein T22F3.7 [Caenorhabditis elegans] ref|NP_504012.1| T22F3.7 [Caenorhabditis elegans] 0.0
blast AAB18311 Hypothetical protein T22F3.8 [Caenorhabditis elegans] ref|NP_504013.1| T22F3.8 [Caenorhabditis elegans] 0.0
blast AAB18309 Serpentine receptor, class h protein 212 [Caenorhabditis elegans] ref|NP_504011.1| Serpentine Receptor, class H family member (srh-212) [Caenorhabditis elegans] 1.0E-178
blast AAD56301 Hypothetical protein T22F3.11a [Caenorhabditis elegans] ref|NP_504015.1| T22F3.11a [Caenorhabditis elegans] 1.0E-166
blast AAB18308 Serpentine receptor, class h protein 213 [Caenorhabditis elegans] ref|NP_504010.1| Serpentine Receptor, class H family member (srh-213) [Caenorhabditis elegans] 1.0E-145
blast AAB18309 Serpentine receptor, class h protein 212 [Caenorhabditis elegans] ref|NP_504011.1| Serpentine Receptor, class H family member (srh-212) [Caenorhabditis elegans] 1.0E-110
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 6.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 6.5E-82
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.7E-118
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504011.1 8 20-42,67-89,101-123,138-160,173-195,200-222,243-265,275-297
T22F3.6 8 20-42,67-89,101-123,138-160,173-195,200-222,243-265,275-297
SNAP00000034228 5 30-49,62-80,84-106,138-160,165-187
GENEFINDER00000034236 19 39-61,95-117,121-143,150-172,182-204,217-236,282-304,317-334,349-371,378-397,412-434,443-465,510-532,544-566,581-603,616-638,643-665,686-708,718-740
Sequence
Target Sequence 
>C. ELEGANS:191517_AT
cgagttccgtggtttattgcccatttaattcttgtcaccgtgttcttgctaccaatctat
tcactgattcccgatcaggaagtggcgaaaaactcgatcagaagaattgctccgtgcatt
ccgctatacgtgaatgttgacttggtttttatcctcttcattgaaactgggcttttcatg
agaacgactggagttcttattttattaggattcattgagacttggattttagcctttatc
acgaatgaacagttgggaaagcagatcaacctgattttgtcgaaaagaactgtcgagttg
cataggaagtttcagaaagcatttatcacacagctaacaattccaatcatcatccttata
attcccataatctatgtaggtgtgaccagtcttttatatttccacaatcaggcaatcaat
aattttattgtgatcattgtgtcgtcccatggatttttctcgacaatcgtgatgatttgc
attcacgtgccgtaccgggagtttacgcttcggtggctcactgtcttggcacatgttcga
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAGTTCCGTGGTTTATTGCCCATT 92 249 421 Antisense
TAATTCTTGTCACCGTGTTCTTGCT 340 635 446 Antisense
GTTCTTGCTACCAATCTATTCACTG 120 445 462 Antisense
TCCGTGCATTCCGCTATACGTGAAT 217 595 531 Antisense
GACTTGGTTTTTATCCTCTTCATTG 535 365 559 Antisense
GACTTGGATTTTAGCCTTTATCACG 506 371 639 Antisense
AACAATTCCAATCATCATCCTTATA 174 135 756 Antisense
GTGTGACCAGTCTTTTATATTTCCA 144 489 800 Antisense
GATCATTGTGTCGTCCCATGGATTT 94 421 852 Antisense
GATGATTTGCATTCACGTGCCGTAC 304 411 891 Antisense
CTCACTGTCTTGGCACATGTTCGAA 289 229 937 Antisense
Sequence Source Affymetrix Proprietary Database