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[191516_at] G-protein coupled receptor

Description T23C6.5 /REP_DB=WormBase Gene ID /WP=CE07530 /TR=O02043 /GB=AAB52881.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_total > 27      4grp_total > 21      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 187.3
(1.2)
P 		195.1
(1.5)
A 		78.7
(-3)
A 		233.4
(3)
A 		155
B 201
(2.6)
A 		164.3
(0.2)
A 		116.9
(-3)
A 		207.1
(3)
A 		90
C 195.2
(0.4)
A 		163.2
(-1.6)
A 		139.6
(-3)
A 		239
(3)
A 		99
avr 194.5
(1.3) 		174.2
(0.3) 		111.7
(-3) 		226.5
(3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 111
1 2 3 4 5 6 range
A 146.2
(3)
A 		88
(-1.9)
A 		103.7
(-0.5)
A 		74.3
(-3)
A 		95.1
(-1.3)
A 		88.4
(-1.8)
A 		72
B 164.1
(3)
A 		120.1
(0.6)
A 		54.3
(-3)
A 		98.9
(-0.6)
A 		87.1
(-1.2)
A 		87.1
(-1.2)
A 		110
C 131.5
(3)
A 		131.3
(3)
A 		122.8
(2.3)
A 		53
(-3)
A 		98.7
(0.5)
A 		109.5
(1.3)
A 		79
avr 147.3
(3) 		113.1
(0.1) 		93.6
(-1.5) 		75.4
(-3) 		93.6
(-1.5) 		95
(-1.4) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 68.2
(3)
P 		42.4
(-0.3)
A 		20.6
(-3)
A 		34.2
(-1.3)
A 		48
B 64.1
(3)
A 		40.6
(-0.8)
A 		27.1
(-3)
A 		44.1
(-0.2)
A 		37
C 64.6
(3)
A 		46.9
(-1.4)
A 		48.7
(-0.9)
A 		40.3
(-3)
A 		24
avr 65.6
(3) 		43.3
(-1) 		32.1
(-3) 		39.5
(-1.7) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 27
(0.9)
A 		31.3
(3)
A 		22.6
(-1.2)
A 		18.8
(-3)
A 		28.7
(1.8)
A 		21.5
(-1.7)
A 		13
B 57.1
(3)
A 		40
(0.7)
A 		13.2
(-3)
A 		26.2
(-1.2)
A 		35.4
(0)
A 		32.5
(-0.4)
A 		44
C 48.7
(3)
A 		40.1
(1.6)
A 		31.3
(0.3)
A 		10.7
(-3)
A 		31.9
(0.3)
A 		36.6
(1.1)
A 		38
avr 44.3
(3) 		37.1
(1.3) 		22.4
(-2.1) 		18.6
(-3) 		32
(0.1) 		30.2
(-0.3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191516_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17977
Sequence Type Exemplar sequence
Representative Public ID T23C6.5 NCBI
Target Description T23C6.5 /REP_DB=WormBase Gene ID /WP=CE07530 /TR=O02043 /GB=AAB52881.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078378(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078378 NCBI Caenorhabditis elegans T23C6.5 (T23C6.5) mRNA, complete cds. 11/11 None
SNAP00000033357 ENSEMBL cdna:SNAP chromosome:CEL140:X:17155207:17157329:-1 11/11 None
GENEFINDER00000033359 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:17155207:17157869:-1 11/11 None
T23C6.5 ENSEMBL cdna:known chromosome:CEL140:X:17155207:17157869:-1 gene:T23C6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:17155206-17157869(-) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Ensembl T23C6.5
Entrez Gene 188781 Entrez gene
SwissProt O02043 EMBL-EBI
WormBase CE35783 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1633531_AT dm
DROSGENOME1:145851_R_AT dm
DROSGENOME1:145850_I_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52881 Hypothetical protein T23C6.5 [Caenorhabditis elegans] ref|NP_510779.2| T23C6.5 [Caenorhabditis elegans] 0.0
blast CAE69697 Hypothetical protein CBG15954 [Caenorhabditis briggsae] 0.0
blast AAB52881 Hypothetical protein T23C6.5 [Caenorhabditis elegans] ref|NP_510779.2| T23C6.5 [Caenorhabditis elegans] 1.0E-173
blast CAE69697 Hypothetical protein CBG15954 [Caenorhabditis briggsae] 1.0E-161
blast CAE59753 Hypothetical protein CBG03199 [Caenorhabditis briggsae] 6.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.2E-34
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.4E-19
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.0E-23
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.1E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510779.2 7 15-37,50-72,92-111,132-154,191-213,255-277,292-314
T23C6.5 7 15-37,50-72,92-111,132-154,191-213,255-277,292-314
SNAP00000033357 6 7-27,37-59,79-101,138-160,202-224,239-261
GENEFINDER00000033359 7 15-37,50-72,92-111,132-154,237-259,301-323,338-360
Sequence
Target Sequence 
>C. ELEGANS:191516_AT
gaagctagtcgccactatcaacttcatcgtctggtacgccgtccctctagtaatccttct
ttgcatctacgctaccatcggcctcgtggtgagcaaggccgcaacaatcacgaaacactc
cgccaacaagcttttgccaagatcaagctggcgatccgaagcctcaaccggcggtgtgtc
agtggagaagaggagaaaggttggtcgtttggcagtcggaatcgtcgtggcattcgcgtt
cttctcactcccgagatatgtatattttatgtggaccgtctggcgagatccgatggcgcc
aagatgtttgaactgccttcaaagtgtactccaacccatcagtttcctgcttctgttctt
taatgccgccgtcgacccattcctctacgcattcctttcaacaagatttcgtcaaagcat
caaagaaacatttcatcttggaaaggtgcgtgaaagcaacatcgagcagtcatcacgagt
gcggcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCTAGTCGCCACTATCAACTTC 199 343 699 Antisense
CTATCAACTTCATCGTCTGGTACGC 677 223 713 Antisense
TAGTAATCCTTCTTTGCATCTACGC 92 653 746 Antisense
TCCGCCAACAAGCTTTTGCCAAGAT 350 595 817 Antisense
AAGCCTCAACCGGCGGTGTGTCAGT 344 147 857 Antisense
TTGGCAGTCGGAATCGTCGTGGCAT 644 707 907 Antisense
GTTCTTCTCACTCCCGAGATATGTA 124 445 936 Antisense
TTATGTGGACCGTCTGGCGAGATCC 663 677 965 Antisense
TGCCTTCAAAGTGTACTCCAACCCA 294 535 1012 Antisense
TTTCCTGCTTCTGTTCTTTAATGCC 640 669 1041 Antisense
TCGAGCAGTCATCACGAGTGCGGCG 156 603 1160 Antisense
Sequence Source Affymetrix Proprietary Database