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[191510_at] CMP-sialic acid transporter

Description ZK896.9 /REP_DB=WormBase Gene ID /WP=CE15465 /TR=O02345 /GB=CAB05326.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=CMP-sialic acid transporter
Cluster 6grp_total > 64      4grp_total > 40      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 50.6
(-3)
A 		101.7
(1.2)
A 		123.9
(3)
M 		88.5
(0.1)
A 		73
B 60
(0.7)
A 		17.6
(-3)
A 		86.6
(3)
A 		52.6
(0)
A 		69
C 7
(-3)
A 		57.1
(3)
A 		36.4
(0.5)
A 		46.7
(1.8)
A 		50
avr 39.2
(-3) 		58.8
(-0.3) 		82.3
(3) 		62.6
(0.3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 4.4
(-3)
A 		6.5
(-2.8)
A 		63.1
(3)
A 		35.1
(0.1)
A 		4.9
(-2.9)
A 		44.1
(1.1)
A 		59
B 52.5
(2.9)
A 		53.4
(3)
A 		12.3
(-2.1)
A 		8
(-2.6)
A 		5
(-3)
A 		9.2
(-2.5)
A 		48
C 5.2
(-3)
A 		13.8
(-1.8)
A 		41.5
(2)
A 		48.7
(3)
A 		26.4
(-0.1)
A 		33.4
(0.9)
A 		44
avr 20.7
(-1.1) 		24.6
(-0.2) 		39
(3) 		30.6
(1.1) 		12.1
(-3) 		28.9
(0.7) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 18.4
(-1.3)
A 		22.1
(-0.2)
A 		32.4
(3)
M 		13
(-3)
A 		19
B 19.1
(2.6)
A 		4.4
(-3)
A 		20.1
(3)
A 		11.2
(-0.4)
A 		16
C 2.3
(-3)
A 		16.4
(3)
A 		12.7
(1.4)
A 		7.9
(-0.6)
A 		14
avr 13.3
(-1.6) 		14.3
(-1) 		21.7
(3) 		10.7
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 0.8
(-3)
A 		2.3
(-2.3)
A 		13.7
(3)
A 		8.9
(0.8)
A 		1.5
(-2.7)
A 		10.7
(1.6)
A 		13
B 18.3
(3)
A 		17.8
(2.8)
A 		3
(-2.6)
A 		2.1
(-3)
A 		2
(-3)
A 		3.4
(-2.5)
A 		16
C 1.9
(-3)
A 		4.2
(-1.5)
A 		10.6
(2.6)
A 		9.8
(2.1)
A 		8.5
(1.3)
A 		11.2
(3)
A 		9
avr 7
(0.5) 		8.1
(1.8) 		9.1
(3) 		6.9
(0.4) 		4
(-3) 		8.4
(2.2) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191510_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9184
Sequence Type Exemplar sequence
Representative Public ID ZK896.9 NCBI
Target Description ZK896.9 /REP_DB=WormBase Gene ID /WP=CE15465 /TR=O02345 /GB=CAB05326.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=CMP-sialic acid transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070047(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070047 NCBI Caenorhabditis elegans ZK896.9 (ZK896.9) mRNA, complete cds. 11/11 None
SNAP00000024382 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12855527:12859344:1 10/11 None
GENEFINDER00000024391 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12855527:12865581:1 10/11 None
ZK896.9 ENSEMBL cdna:known chromosome:CEL140:IV:12855527:12859344:1 gene:ZK896.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12855532-12859350(+) 100.0 100.0
Public Domain and Genome References
Gene Title CMP-sialic acid transporter
Ensembl ZK896.9
Entrez Gene 191452 Entrez gene
SwissProt O02345 EMBL-EBI
WormBase CE15465 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8643 carbohydrate transport inferred from electronic annotation QuickGO AmiGO
15780 nucleotide-sugar transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
139 Golgi membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5338 nucleotide-sugar transporter activity inferred from electronic annotation QuickGO AmiGO
5351 sugar porter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05326 Hypothetical protein ZK896.9 [Caenorhabditis elegans] ref|NP_502448.1| ZK896.9 [Caenorhabditis elegans] 0.0
blast CAB05325 Hypothetical protein ZK896.8 [Caenorhabditis elegans] ref|NP_502449.1| Guanylyl CYclase family member (gcy-18) [Caenorhabditis elegans] 0.0
blast CAE56618 Hypothetical protein CBG24374 [Caenorhabditis briggsae] 0.0
blast NP_500309 Guanylyl CYclase family member (gcy-23) [Caenorhabditis elegans] gb|AAC17555.3| Guanylyl cyclase protein 23 [Caenorhabditis elegans] 0.0
blast CAE56617 Hypothetical protein CBG24373 [Caenorhabditis briggsae] 1.0E-166
blast CAB05326 Hypothetical protein ZK896.9 [Caenorhabditis elegans] ref|NP_502448.1| ZK896.9 [Caenorhabditis elegans] 1.0E-111
blast CAE56617 Hypothetical protein CBG24373 [Caenorhabditis briggsae] 2.0E-71
blast EAL31692 GA15422-PA [Drosophila pseudoobscura] 2.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR007271 EMBL-EBI Nucleotide-sugar transporter 1.0E-126
Pfam IPR007271 EMBL-EBI Nucleotide-sugar transporter 2.7E-57
Pfam IPR007271 EMBL-EBI Nucleotide-sugar transporter 5.1E-42
Pfam IPR000719 EMBL-EBI Protein kinase 5.8E-10
Pfam IPR001054 EMBL-EBI Guanylate cyclase 4.0E-71
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 1.5E-60
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502448.1 9 13-32,88-106,111-133,138-155,210-232,242-264,290-310,320-337,342-364
ZK896.9 9 13-32,88-106,111-133,138-155,210-232,242-264,290-310,320-337,342-364
SNAP00000024382 6 13-32,111-133,142-159,208-227,234-256,260-282
GENEFINDER00000024391 11 13-32,88-106,111-133,138-155,168-190,210-232,245-264,290-309,316-338,343-362,893-915
Sequence
Target Sequence 
>C. ELEGANS:191510_AT
ttcggtgctcggatttatcgccgttctcatcgcttgcgttctatccggatttgctggaat
ctatttcgagaagattctcaaaggttcaaatgtgtcgatttggatcagaaatatccagct
ggcgttcccatcgattttcttcgcttttctctttgcatcggtaaaagacaattcaagcct
ctaccaagacggcccgaacccaatcgaaatctggaataacatgcttcaaggattcgactg
ggcagtctgggttactgtagccatcaacgcattcggaggactcgttgttgcagtcgtcat
caagtatgctgataatattctcaaggcatttgcaacatcacttgccatcgttctcaactg
tatcgctgcttacttcctgttcaacttccgtccatcgattctcttcctcgtcggagcttc
tggtgttatcgcagccgtcttcgcctatagcatgtacccgtacaaagcctctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGGTGCTCGGATTTATCGCCGTT 8 663 642 Antisense
TTGCGTTCTATCCGGATTTGCTGGA 681 699 675 Antisense
AATTCAAGCCTCTACCAAGACGGCC 8 171 811 Antisense
GGCCCGAACCCAATCGAAATCTGGA 405 541 832 Antisense
GCTTCAAGGATTCGACTGGGCAGTC 45 305 864 Antisense
GTCTGGGTTACTGTAGCCATCAACG 34 473 886 Antisense
GCCATCAACGCATTCGGAGGACTCG 422 279 901 Antisense
TGCAACATCACTTGCCATCGTTCTC 330 577 972 Antisense
TCTCAACTGTATCGCTGCTTACTTC 433 613 993 Antisense
TCGTCGGAGCTTCTGGTGTTATCGC 172 601 1049 Antisense
GCATGTACCCGTACAAAGCCTCTCA 464 309 1091 Antisense
Sequence Source Affymetrix Proprietary Database