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[191504_at] sulphate adenyltransferase

Description T14G10.1 /REP_DB=WormBase Gene ID /WP=CE06447 /TR=Q22501 /GB=CAA93098.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=sulphate adenyltransferase
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 08      4grp_high_8_8 > 52      6grp_highest_nonorm > 75      6grp_total > 63      4grp_total > 59      6grp_highest_sub > 03      6grp_cgc > 93      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 441
1 2 3 4 range
A 862.7
(0.4)
P 		917.1
(1.4)
P 		1002
(3)
P 		676.2
(-3)
P 		326
B 895.6
(-0.7)
P 		1112.2
(3)
P 		786.2
(-2.6)
P 		762.5
(-3)
P 		350
C 866.1
(-0.3)
P 		1058.5
(2.4)
P 		1103.9
(3)
P 		671.6
(-3)
P 		432
avr 874.8
(0.2) 		1029.3
(3) 		964
(1.8) 		703.4
(-3) 		326

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 551
1 2 3 4 5 6 range
A 842.4
(-1.1)
P 		872.3
(-0.8)
P 		1116.5
(1.9)
P 		1217.2
(3)
P 		671.9
(-3)
P 		710.9
(-2.6)
P 		545
B 789.7
(-2.2)
P 		792
(-2.1)
P 		1211.4
(3)
P 		1035.4
(0.8)
P 		722.6
(-3)
P 		945.8
(-0.3)
P 		489
C 849.5
(-2)
P 		844.3
(-2.1)
P 		1222.4
(3)
P 		1094.5
(1.3)
P 		773.3
(-3)
P 		1044.9
(0.6)
P 		449
avr 827.2
(-1.6) 		836.2
(-1.5) 		1183.4
(3) 		1115.7
(2.1) 		722.6
(-3) 		900.5
(-0.7) 		461
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 286
1 2 3 4 range
A 314.1
(3)
P 		199.1
(-0.2)
P 		262
(1.5)
P 		99
(-3)
P 		215
B 285.6
(3)
P 		274.7
(2.5)
P 		182.2
(-2)
P 		162.5
(-3)
P 		123
C 286.7
(0.8)
P 		304.3
(1.2)
P 		384.7
(3)
P 		113.2
(-3)
P 		272
avr 295.5
(3) 		259.4
(1.7) 		276.3
(2.3) 		124.9
(-3) 		171

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 197
1 2 3 4 5 6 range
A 155.3
(-3)
P 		310.2
(3)
P 		243.2
(0.4)
P 		308.7
(2.9)
P 		202.8
(-1.2)
P 		173.1
(-2.3)
P 		155
B 274.8
(-2.2)
P 		263.6
(-3)
P 		294.2
(-0.9)
P 		274.2
(-2.3)
P 		293.6
(-1)
P 		352.7
(3)
P 		89
C 314.9
(1.4)
P 		257.6
(-1.3)
P 		311.1
(1.2)
P 		220.1
(-3)
P 		250.2
(-1.6)
P 		349.8
(3)
P 		130
avr 248.3
(-3) 		277.1
(1) 		282.8
(1.7) 		267.7
(-0.3) 		248.9
(-2.9) 		291.9
(3) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191504_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8493
Sequence Type Exemplar sequence
Representative Public ID T14G10.1 NCBI
Target Description T14G10.1 /REP_DB=WormBase Gene ID /WP=CE06447 /TR=Q22501 /GB=CAA93098.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=sulphate adenyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069456(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069456 NCBI Caenorhabditis elegans 3'-Phosphoadenosine 5'-Phosphosulfate Synthetase family member (pps-1) (pps-1) mRNA, complete cds. 11/11 None
SNAP00000034623 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10161956:10164226:1 11/11 None
GENEFINDER00000034625 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10162001:10164226:1 11/11 None
T14G10.1.1 ENSEMBL cdna:known chromosome:CEL140:IV:10161979:10164352:1 gene:T14G10.1 11/11 None
T14G10.1.2 ENSEMBL cdna:known chromosome:CEL140:IV:10161979:10164409:1 gene:T14G10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10162006-10164232(+) 100.0 100.0
Public Domain and Genome References
Gene Title sulphate adenyltransferase
Gene Symbol pps-1
Ensembl T14G10.1
Entrez Gene 177893 Entrez gene
SwissProt Q22501 EMBL-EBI
WormBase CE06447 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256835_AT sulfate adenylyltransferase 1 / ATP-sulfurylase 1 (APS1) at
CANINE_2:CFA.17339.1.S1_S_AT similar to 3-phosphoadenosine 5-phosphosulfate synthase 2 isoform b cfa
CANINE_2:CFAAFFX.23995.1.S1_AT similar to 3-phosphoadenosine 5-phosphosulfate synthase 2 isoform b cfa
DROSOPHILA_2:1634522_S_AT PAPS synthetase dm
DROSGENOME1:154327_AT PAPS synthetase dm
HG-U133A:203059_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133_PLUS_2:203059_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133A_2:203059_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133A:203060_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133_PLUS_2:203060_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133A_2:203060_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HU35KSUBB:RC_W47175_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
U133_X3P:G5052074_3P_A_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
U133_X3P:G4758879_3P_A_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
U133_X3P:HS.274230.0.S2_3P_A_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133A_2:203058_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-FOCUS:203058_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133_PLUS_2:203058_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133A:203058_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U95AV2:36233_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U95C:60873_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U95C:60874_G_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U95C:63882_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133_PLUS_2:237496_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U133B:237496_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
HG-U95B:43025_R_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
U133_X3P:HS.227505.0.A1_3P_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 hs
MOUSE430_2:1421987_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOE430A:1421987_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOUSE430A_2:1421987_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOUSE430_2:1421988_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOUSE430A_2:1421988_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOE430A:1421988_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOE430A:1421989_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOUSE430A_2:1421989_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOUSE430_2:1421989_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MU11KSUBA:AA242579_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MG-U74AV2:96713_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOE430A:1434510_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOUSE430A_2:1434510_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MOUSE430_2:1434510_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 mm
MU19KSUBB:TC29597_S_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), mRNA mm
MU19KSUBB:TC34127_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), mRNA mm
MU19KSUBB:TC34694_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), mRNA mm
RAE230B:1395721_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (predicted) rn
RAT230_2:1395721_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (predicted) rn
RAE230A:1376047_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (predicted) rn
RAT230_2:1376047_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (predicted) rn
RG-U34A:RC_AA893244_AT 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (predicted) rn
YEAST_2:1774365_AT Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
103 sulfate assimilation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4781 sulfate adenylyltransferase (ATP) activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
16301 kinase activity inferred from electronic annotation QuickGO AmiGO
16772 transferase activity, transferring phosphorus-containing groups inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93098 Hypothetical protein T14G10.1 [Caenorhabditis elegans] ref|NP_501857.1| 3'-Phosphoadenosine 5'-Phosphosulfate Synthetase family member (pps-1) [Caenorhabditis elegans] 0.0
blast CAE59919 Hypothetical protein CBG03405 [Caenorhabditis briggsae] 0.0
blast AAT39125 PAPS synthase 2 [Oryctolagus cuniculus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002650 EMBL-EBI ATP-sulfurylase 1.0E-126
Pfam IPR002891 EMBL-EBI Adenylylsulfate kinase 1.3E-125
Pfam IPR002650 EMBL-EBI ATP-sulfurylase 1.0E-126
Pfam IPR002891 EMBL-EBI Adenylylsulfate kinase 1.3E-125
Sequence
Target Sequence 
>C. ELEGANS:191504_AT
gattcataacggacatgctcttctaatgagagatacccgtgaaaaattgctcgctgagca
caaaaatccaattcttttattgcacccacttggaggatggacaaaggatgatgatgtacc
cttggatattagaataaaacaacacgaagcagttattgctgaaagagttttggatccaga
atggactgttctctcgattttcccatctccaatgatgtatgctggtccaactgaagttca
gtggcatgccagatccagaatagctgctggtattcaacactatatagtgggacgtgatcc
tgctggaattcaaaaaccaggaagtcctgatgctctttatgaaactacacatggagctaa
agtactttcaatggctccaggtctttctgctcttcatatacttccattccgagttgctgc
atatgataaaacagccaagaaaatgtcatttttcgatacatcaagaaaagaagattttga
aaacattagtggaacaaagatgcgaggattggcacgaaatggagatacaccaccagaggg
attcatggctccaactgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCATAACGGACATGCTCTTCTA 303 431 1365 Antisense
GATACCCGTGAAAAATTGCTCGCTG 473 423 1396 Antisense
TTCTTTTATTGCACCCACTTGGAGG 672 687 1436 Antisense
AATGGACTGTTCTCTCGATTTTCCC 650 161 1544 Antisense
CAATGATGTATGCTGGTCCAACTGA 240 177 1574 Antisense
GTGGCATGCCAGATCCAGAATAGCT 611 491 1605 Antisense
GTGGGACGTGATCCTGCTGGAATTC 208 489 1651 Antisense
CCAGGAAGTCCTGATGCTCTTTATG 120 273 1681 Antisense
TTTCAATGGCTCCAGGTCTTTCTGC 95 671 1730 Antisense
ATATACTTCCATTCCGAGTTGCTGC 600 19 1760 Antisense
CAGAGGGATTCATGGCTCCAACTGC 639 203 1898 Antisense
Sequence Source Affymetrix Proprietary Database