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[191489_s_at]

Description ZK524.2A /REP_DB=WormBase Gene ID /WP=CE15371 /GEN=unc-13 /TR=O17665 /GB=CAA98147.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=UNC-13 protein
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 13      4grp_highest_8_8 > 52      4grp_high_8_8 > 25      6grp_highest_nonorm > 30      6grp_total > 14      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1162
1 2 3 4 range
A 1499.1
(3)
P 		346.8
(-2.9)
P 		337.1
(-3)
P 		706.4
(-1.1)
P 		1162
B 1098.6
(3)
P 		502.6
(-1.8)
P 		353.5
(-3)
P 		733.6
(0.1)
P 		745
C 1450.3
(3)
P 		524.6
(-2.1)
P 		368.1
(-3)
P 		563.4
(-1.9)
P 		1082
avr 1349.3
(3) 		458
(-2.4) 		352.9
(-3) 		667.8
(-1.1) 		996

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1055
1 2 3 4 5 6 range
A 890.5
(1.2)
P 		1099.1
(3)
P 		677.4
(-0.7)
P 		788.1
(0.3)
P 		500.5
(-2.2)
P 		412.8
(-3)
P 		686
B 1419.1
(3)
P 		1153
(1.5)
P 		750.5
(-0.8)
P 		784.2
(-0.6)
P 		453.9
(-2.5)
P 		363.8
(-3)
P 		1055
C 1347.8
(3)
P 		1116.6
(1.5)
P 		793.4
(-0.5)
P 		937.2
(0.4)
P 		509.7
(-2.3)
P 		402.7
(-3)
P 		945
avr 1219.1
(3) 		1122.9
(2.3) 		740.4
(-0.5) 		836.5
(0.2) 		488
(-2.3) 		393.1
(-3) 		826
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 471
1 2 3 4 range
A 545.8
(3)
P 		75.3
(-3)
P 		88.1
(-2.8)
P 		103.4
(-2.6)
P 		471
B 350.3
(3)
P 		124.1
(-2.1)
P 		81.9
(-3)
P 		156.3
(-1.3)
P 		268
C 480.1
(3)
P 		150.8
(-2.1)
P 		128.3
(-2.5)
P 		95
(-3)
P 		385
avr 458.7
(3) 		116.7
(-2.7) 		99.4
(-3) 		118.2
(-2.7) 		359

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 399
1 2 3 4 5 6 range
A 164.2
(-1.7)
P 		390.9
(3)
P 		147.5
(-2)
P 		199.9
(-0.9)
P 		151
(-2)
P 		100.5
(-3)
P 		290
B 493.8
(3)
P 		383.8
(1.2)
P 		182.2
(-2.2)
P 		207.7
(-1.8)
P 		184.4
(-2.2)
P 		135.7
(-3)
P 		358
C 499.6
(3)
P 		340.7
(0.4)
P 		201.9
(-1.9)
P 		188.5
(-2.1)
P 		164.9
(-2.5)
P 		134.8
(-3)
P 		365
avr 385.9
(3) 		371.8
(2.7) 		177.2
(-1.8) 		198.7
(-1.3) 		166.8
(-2) 		123.7
(-3) 		262
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191489_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1529
Sequence Type Exemplar sequence
Representative Public ID ZK524.2A NCBI
Target Description ZK524.2A /REP_DB=WormBase Gene ID /WP=CE15371 /GEN=unc-13 /TR=O17665 /GB=CAA98147.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=UNC-13 protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026703(11), NM_001026702(11), NM_001026700(11), NM_001026701(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026703 NCBI Caenorhabditis elegans UNCoordinated family member (unc-13) (unc-13) mRNA, complete cds. 11/11 None
NM_001026702 NCBI Caenorhabditis elegans UNCoordinated family member (unc-13) (unc-13) mRNA, complete cds. 11/11 None
NM_001026700 NCBI Caenorhabditis elegans UNCoordinated family member (unc-13) (unc-13) mRNA, complete cds. 11/11 None
NM_001026701 NCBI Caenorhabditis elegans UNCoordinated family member (unc-13) (unc-13) mRNA, complete cds. 11/11 None
GENEFINDER00000017083 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7453508:7462030:1 11/11 None
SNAP00000017079 ENSEMBL cdna:SNAP chromosome:CEL140:I:7458440:7461857:1 11/11 None
ZK524.2d ENSEMBL cdna:known chromosome:CEL140:I:7430913:7461857:1 gene:ZK524.2 11/11 A 
ZK524.2e ENSEMBL cdna:known chromosome:CEL140:I:7430913:7462216:1 gene:ZK524.2 11/11 A 
ZK524.2a ENSEMBL cdna:known chromosome:CEL140:I:7430913:7461857:1 gene:ZK524.2 11/11 A 
ZK524.2c ENSEMBL cdna:known chromosome:CEL140:I:7441545:7461857:1 gene:ZK524.2 11/11 A 
ZK524.2b ENSEMBL cdna:known chromosome:CEL140:I:7451297:7461857:1 gene:ZK524.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7430843-7461788(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK524.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE11299 Hypothetical protein ZK524.2e [Caenorhabditis elegans] emb|CAE11317.1| Hypothetical protein ZK524.2e [Caenorhabditis elegans] emb|CAE11294.1| Hypothetical protein ZK524.2e [Caenorhabditis elegans] ref|NP_001021874.1| UNCoordinated family member (unc-13) [Caenorhabditis elegans] 0.0
blast CAB01966 Hypothetical protein ZK524.2a [Caenorhabditis elegans] emb|CAA98147.1| Hypothetical protein ZK524.2a [Caenorhabditis elegans] emb|CAB07173.1| Hypothetical protein ZK524.2a [Caenorhabditis elegans] ref|NP_001021871.1| UNCoordinated family member (unc-13) [Caenorhabditis elegans] 0.0
blast AAA93094 UNC-13 0.0
blast CAD90170 Hypothetical protein ZK524.2d [Caenorhabditis elegans] emb|CAD90190.2| Hypothetical protein ZK524.2d [Caenorhabditis elegans] emb|CAD90173.2| Hypothetical protein ZK524.2d [Caenorhabditis elegans] ref|NP_001021873.1| UNCoordinated family member (unc-13) [Caenorhabditis elegans] sp|P27715|UNC13_CAEEL Phorbol ester/diacylglycerol-binding protein unc-13 (Uncoordinated protein 13) 0.0
blast CAD56619 Hypothetical protein ZK524.2c [Caenorhabditis elegans] emb|CAD56561.2| Hypothetical protein ZK524.2c [Caenorhabditis elegans] ref|NP_001021872.1| UNCoordinated family member (unc-13) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 9.0E-79
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 2.8E-74
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.1E-23
Pfam IPR000008 EMBL-EBI C2 domain 2.1E-6
Pfam IPR000008 EMBL-EBI C2 domain 5.8E-32
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-17
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 9.0E-79
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 2.8E-74
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.1E-23
Pfam IPR000008 EMBL-EBI C2 domain 5.8E-32
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-17
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 9.0E-79
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 2.8E-74
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.1E-23
Pfam IPR000008 EMBL-EBI C2 domain 5.8E-32
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-17
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 9.0E-79
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 2.8E-74
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.1E-23
Pfam IPR000008 EMBL-EBI C2 domain 5.8E-32
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-17
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 9.0E-79
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 2.8E-74
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.1E-23
Pfam IPR000008 EMBL-EBI C2 domain 5.8E-32
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-17
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 3.0E-35
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 2.8E-74
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-17
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 9.0E-79
Pfam IPR010439 EMBL-EBI Protein of unknown function DUF1041 2.8E-74
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.1E-23
Pfam IPR000008 EMBL-EBI C2 domain 5.8E-32
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-17
Sequence
Target Sequence 
>C. ELEGANS:191489_S_AT
gacttattcagtcatccaggcactggggaacagaaggttaccgttaaaatccttgccgca
aacgatcttcgatggcaaacctcgtcagctttcaaaccatttgttgaagtgcatcttgtc
ggaccacatctttcagacaaaaagagaaaatggtcgacaaagaccaaagcaggcaactgg
gctccaaaattcaacgaaacttttcatttcttccttggaaatgaaggagaacctgaacat
tacgagcttatgtttcaagtcaaggattactgttttgcacgagatgatcgagttgttgga
gttggagttctgcagttatcatctgtcgttgatcaagcgggatcctgcgccatgtgggtt
caacttggaactcgactgcatatcgacgagactgggctcattttgctcaggattctttca
cagcgtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTATTCAGTCATCCAGGCACTG 116 373 4918 Antisense
CCTTGCCGCAAACGATCTTCGATGG 632 269 4968 Antisense
TCTTCGATGGCAAACCTCGTCAGCT 590 617 4983 Antisense
CCTCGTCAGCTTTCAAACCATTTGT 536 267 4997 Antisense
TCTTGTCGGACCACATCTTTCAGAC 259 617 5031 Antisense
GACCAAAGCAGGCAACTGGGCTCCA 21 383 5079 Antisense
GAGTTCTGCAGTTATCATCTGTCGT 166 399 5222 Antisense
ATCCTGCGCCATGTGGGTTCAACTT 556 39 5259 Antisense
TCAACTTGGAACTCGACTGCATATC 255 629 5277 Antisense
GACGAGACTGGGCTCATTTTGCTCA 569 377 5302 Antisense
TGCTCAGGATTCTTTCACAGCGTCA 133 581 5321 Antisense
Sequence Source Affymetrix Proprietary Database