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[191486_at] nucleotide translocator

Description F55A11.4 /REP_DB=WormBase Gene ID /WP=CE05946 /TR=SW:Q20799 /GB=CAA96658.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=nucleotide translocator
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 96      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 91      6grp_total > 87      4grp_total > 54      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 135.6
(2.5)
P 		131.6
(1.8)
P 		105
(-3)
P 		138.6
(3)
P 		34
B 171.6
(3)
P 		168
(2.4)
P 		165.3
(2)
P 		133.6
(-3)
P 		38
C 204.7
(3)
P 		171.7
(-1.5)
P 		182.3
(-0)
P 		160.4
(-3)
P 		44
avr 170.6
(3) 		157.1
(-0.1) 		150.9
(-1.5) 		144.2
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 147.3
(-2.3)
P 		146
(-2.3)
P 		163.7
(-1.6)
P 		129.6
(-3)
P 		187.5
(-0.6)
P 		274.5
(3)
P 		145
B 99.5
(-3)
P 		148.7
(-1.5)
P 		112.3
(-2.6)
P 		164.1
(-1)
P 		246.7
(1.5)
P 		297
(3)
P 		198
C 105.9
(-2.8)
P 		99.5
(-3)
P 		214.2
(0.3)
P 		161.5
(-1.2)
P 		204.5
(0)
P 		309.4
(3)
P 		210
avr 117.6
(-3) 		131.4
(-2.5) 		163.4
(-1.4) 		151.7
(-1.8) 		212.9
(0.2) 		293.6
(3) 		176
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 49.4
(3)
P 		28.6
(-1.3)
P 		27.4
(-1.5)
P 		20.3
(-3)
P 		29
B 54.7
(3)
P 		41.5
(-0)
P 		38.3
(-0.8)
P 		28.5
(-3)
P 		26
C 67.8
(3)
P 		49.4
(0.3)
P 		63.6
(2.4)
P 		27
(-3)
P 		41
avr 57.3
(3) 		39.8
(-0.3) 		43.1
(0.3) 		25.3
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 27.1
(-3)
P 		51.9
(0.7)
P 		35.7
(-1.7)
P 		32.9
(-2.1)
P 		56.6
(1.5)
P 		66.8
(3)
P 		40
B 34.6
(-2.5)
P 		49.5
(-1.4)
P 		27.3
(-3)
P 		43.5
(-1.8)
P 		100.2
(2.2)
P 		110.8
(3)
P 		84
C 39.2
(-2.3)
P 		30.4
(-3)
P 		54.5
(-1)
P 		32.5
(-2.8)
P 		66.2
(-0.1)
P 		103.6
(3)
P 		73
avr 33.6
(-3) 		43.9
(-2) 		39.2
(-2.4) 		36.3
(-2.7) 		74.3
(1.1) 		93.7
(3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191486_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13011
Sequence Type Exemplar sequence
Representative Public ID F55A11.4 NCBI
Target Description F55A11.4 /REP_DB=WormBase Gene ID /WP=CE05946 /TR=SW:Q20799 /GB=CAA96658.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=nucleotide translocator
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073569(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073569 NCBI Caenorhabditis elegans F55A11.4 (F55A11.4) mRNA, complete cds. 11/11 None
SNAP00000030956 ENSEMBL cdna:SNAP chromosome:CEL140:V:11773844:11775757:1 9/11 None
GENEFINDER00000030965 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11773844:11779444:1 9/11 None
F55A11.4 ENSEMBL cdna:known chromosome:CEL140:V:11773844:11776073:1 gene:F55A11.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11773843-11776073(+) 100.0 100.0
Public Domain and Genome References
Gene Title nucleotide translocator
Ensembl F55A11.4
Entrez Gene 186276 Entrez gene
SwissProt Q20799 EMBL-EBI
WormBase CE05946 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HU35KSUBA:RC_AA179338_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
HU35KSUBA:RC_AA481862_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
HU35KSUBD:RC_T32561_S_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
U133_X3P:HS.5476.0.A1_3P_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
HG-U133_PLUS_2:225212_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
HG-U133B:225212_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
HG-U95B:53085_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
HG-U95B:53087_G_AT solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
HU35KSUBC:RC_D25976_AT Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 hs
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5743 mitochondrial inner membrane inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5488 binding inferred from electronic annotation QuickGO AmiGO
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA96658 Hypothetical protein F55A11.4 [Caenorhabditis elegans] ref|NP_505970.1| F55A11.4 [Caenorhabditis elegans] sp|Q20799|CMC2_CAEEL Probable calcium-binding mitochondrial carrier F55A11.4 0.0
blast CAE75300 Hypothetical protein CBG23270 [Caenorhabditis briggsae] 0.0
blast CAE57255 Hypothetical protein CBG00135 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001993 EMBL-EBI Mitochondrial substrate carrier 2.3E-36
Pfam IPR001993 EMBL-EBI Mitochondrial substrate carrier 4.1E-33
Pfam IPR001993 EMBL-EBI Mitochondrial substrate carrier 2.7E-26
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.3E-4
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 6.9E-6
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.048
Pfam IPR001993 EMBL-EBI Mitochondrial substrate carrier 1.7E-26
Pfam IPR002123 EMBL-EBI Phospholipid/glycerol acyltransferase 6.0E-36
Sequence
Target Sequence 
>C. ELEGANS:191486_AT
gaggaatcatacatttcgcgcacaaaatgtacaccaaggaaggtatacgatgcttttaca
aaggatacttgccaaatctaattggtattattccttatgctggaatcgatttagcaattt
acgaaacattaaaacgaacatacgtaagatattacgagacaaactcctcagaacccggcg
ttttagctctccttgcttgtggaacgtgtagctcaacatgtggacaactttctagctacc
catttgcactagttcgaacaaggctgcaagctttatctatcacacgttattccccacagc
ctgatacgatgtttggacagttcaaatatatactacagaacgaaggagtcactggattct
atagaggcattacacctaattttttaaaggttattccggcagtcagcatcagttatgttg
tttacgaaaaagtgcggacgggtctcggcgttcccgtatgctcacgaggaggcctcgagg
atattcatcagttcttgccatgttcgattcattccataatacaattctttttttttccac
gaactttcttgttgaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGAATCATACATTTCGCGCACAA 538 405 1157 Antisense
TACGAGACAAACTCCTCAGAACCCG 585 647 1309 Antisense
TTAGCTCTCCTTGCTTGTGGAACGT 7 671 1339 Antisense
CATGTGGACAACTTTCTAGCTACCC 197 209 1373 Antisense
TAGCTACCCATTTGCACTAGTTCGA 598 651 1389 Antisense
GGCTGCAAGCTTTATCTATCACACG 64 541 1418 Antisense
ATTCCCCACAGCCTGATACGATGTT 520 7 1445 Antisense
ATTCCGGCAGTCAGCATCAGTTATG 347 7 1549 Antisense
GAAAAAGTGCGGACGGGTCTCGGCG 431 355 1582 Antisense
ATCAGTTCTTGCCATGTTCGATTCA 563 29 1643 Antisense
TTTTCCACGAACTTTCTTGTTGACA 34 675 1689 Antisense
Sequence Source Affymetrix Proprietary Database