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[191483_at] Ras-like protein

Description F17C8.4 /REP_DB=WormBase Gene ID /WP=CE00958 /GEN=ras-2 /TR=Q19524 /GB=CAA84796.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ras-like protein
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 61      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_highest_nonorm > 45      6grp_total > 61      4grp_total > 18      6grp_highest_sub > 04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 897
1 2 3 4 range
A 1566.2
(-0.9)
P 		1962.4
(1.9)
P 		1274.3
(-3)
P 		2113.5
(3)
P 		839
B 1575.6
(-3)
P 		1761.5
(-0.1)
P 		1723.1
(-0.7)
P 		1963.3
(3)
P 		388
C 1570.8
(-2.2)
P 		1477.9
(-3)
P 		1690
(-1.2)
P 		2171.6
(3)
P 		694
avr 1570.9
(-2.9) 		1733.9
(-1) 		1562.5
(-3) 		2082.8
(3) 		520

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1745
1 2 3 4 5 6 range
A 1975.5
(-0.4)
P 		1948.5
(-0.5)
P 		2720
(2.5)
P 		2857.8
(3)
P 		2066.3
(-0)
P 		1297.5
(-3)
P 		1560
B 2065.7
(-1)
P 		2311.8
(0)
P 		3042.8
(3)
P 		2541.8
(1)
P 		2254.8
(-0.2)
P 		1558.6
(-3)
P 		1484
C 1619.5
(-3)
P 		2152.7
(-0.4)
P 		2357.7
(0.5)
P 		2872.9
(3)
P 		1815.5
(-2.1)
P 		1711.3
(-2.6)
P 		1253
avr 1886.9
(-1.2) 		2137.7
(-0) 		2706.8
(2.8) 		2757.5
(3) 		2045.5
(-0.5) 		1522.5
(-3) 		1235
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 280
1 2 3 4 range
A 570.2
(3)
P 		426
(-0.3)
P 		333.1
(-2.5)
P 		309.4
(-3)
P 		261
B 502.5
(3)
P 		435.1
(-0.9)
P 		399.3
(-3)
P 		418.4
(-1.9)
P 		103
C 520
(1.1)
P 		424.9
(-1.4)
P 		589
(3)
P 		366.2
(-3)
P 		223
avr 530.9
(3) 		428.7
(-0.7) 		440.5
(-0.3) 		364.7
(-3) 		166

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 600
1 2 3 4 5 6 range
A 364.2
(-2.3)
P 		693
(2.5)
P 		592.4
(1.1)
P 		724.8
(3)
P 		623.5
(1.5)
P 		315.9
(-3)
P 		409
B 718.8
(-0.5)
P 		769.5
(0.4)
P 		738.9
(-0.2)
P 		673.2
(-1.4)
P 		916.1
(3)
P 		581.2
(-3)
P 		335
C 600.3
(-1)
P 		656.9
(3)
P 		600.1
(-1.1)
P 		577.7
(-2.7)
P 		587.5
(-2)
P 		572.9
(-3)
P 		84
avr 561.1
(-1.1) 		706.5
(2.9) 		643.8
(1.2) 		658.6
(1.6) 		709
(3) 		490
(-3) 		219
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191483_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6701
Sequence Type Exemplar sequence
Representative Public ID F17C8.4 NCBI
Target Description F17C8.4 /REP_DB=WormBase Gene ID /WP=CE00958 /GEN=ras-2 /TR=Q19524 /GB=CAA84796.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ras-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065571(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065571 NCBI Caenorhabditis elegans R-RAS related family member (ras-2) (ras-2) mRNA, complete cds. 11/11 None
SNAP00000012146 ENSEMBL cdna:SNAP chromosome:CEL140:III:4737681:4740027:-1 11/11 None
GENEFINDER00000012151 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4737681:4740027:-1 11/11 None
F17C8.4.3 ENSEMBL cdna:known chromosome:CEL140:III:4737564:4740039:-1 gene:F17C8.4 11/11 None
F17C8.4.1 ENSEMBL cdna:known chromosome:CEL140:III:4737648:4740037:-1 gene:F17C8.4 11/11 None
F17C8.4.2 ENSEMBL cdna:known chromosome:CEL140:III:4737681:4740030:-1 gene:F17C8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4737680-4740027(-) 100.0 100.0
Public Domain and Genome References
Gene Title Ras-like protein
Gene Symbol ras-2
Ensembl F17C8.4
Entrez Gene 175625 Entrez gene
SwissProt Q19524 EMBL-EBI
WormBase CE00958 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1603437_AT similar to Ras-related protein M-Ras (Ras-related protein R-Ras3) cfa
CANINE_2:CFAAFFX.12164.1.S1_AT similar to Ras-related protein M-Ras (Ras-related protein R-Ras3) cfa
CHICKEN:GGA.5500.2.S1_A_AT muscle RAS oncogene homolog gga
HG-U133_PLUS_2:206538_AT muscle RAS oncogene homolog hs
HG-U133A:206538_AT muscle RAS oncogene homolog hs
HG-FOCUS:206538_AT muscle RAS oncogene homolog hs
HG-U133A_2:206538_AT muscle RAS oncogene homolog hs
HU35KSUBB:RC_T23825_AT muscle RAS oncogene homolog hs
HU35KSUBD:RC_AA450261_AT muscle RAS oncogene homolog hs
U133_X3P:G6912513_3P_X_AT muscle RAS oncogene homolog hs
U133_X3P:G6912513_3P_AT muscle RAS oncogene homolog hs
U133_X3P:HS.7298.1.A1_3P_AT muscle RAS oncogene homolog hs
HG-U133_PLUS_2:225185_AT muscle RAS oncogene homolog hs
HG-U133B:225185_AT muscle RAS oncogene homolog hs
HG-U95AV2:38540_AT muscle RAS oncogene homolog hs
HG-U95B:57217_AT muscle RAS oncogene homolog hs
MOUSE430A_2:1449590_A_AT muscle and microspikes RAS mm
MOUSE430_2:1449590_A_AT muscle and microspikes RAS mm
MOE430A:1449590_A_AT muscle and microspikes RAS mm
MU11KSUBA:AB004879_AT muscle and microspikes RAS mm
MU11KSUBB:MSA.22537.0_S_AT muscle and microspikes RAS mm
MG-U74AV2:103653_AT muscle and microspikes RAS mm
MG-U74BV2:164306_F_AT muscle and microspikes RAS mm
MU19KSUBA:TC15801_S_AT Muscle and microspikes RAS, mRNA (cDNA clone MGC:36159 IMAGE:4973582) mm
MU19KSUBA:TC15948_AT Muscle and microspikes RAS, mRNA (cDNA clone MGC:36159 IMAGE:4973582) mm
MU19KSUBA:TC21420_AT Muscle and microspikes RAS, mRNA (cDNA clone MGC:36159 IMAGE:4973582) mm
RG-U34A:D89863_G_AT muscle and microspikes RAS rn
RG-U34A:D89863_AT muscle and microspikes RAS rn
RG-U34C:RC_AI235437_AT muscle and microspikes RAS rn
RAE230A:1387368_AT muscle and microspikes RAS rn
RAT230_2:1387368_AT muscle and microspikes RAS rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84796 Hypothetical protein F17C8.4 [Caenorhabditis elegans] ref|NP_497972.1| R-RAS related family member (ras-2) [Caenorhabditis elegans] gb|AAB38969.1| GTP binding protein [Caenorhabditis elegans] 1.0E-117
blast CAE71142 Hypothetical protein CBG17997 [Caenorhabditis briggsae] 1.0E-114
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.4E-84
Sequence
Target Sequence 
>C. ELEGANS:191483_AT
agagaccgccagaggatgattccaaactaccatattacaaattggtagtgatcggagatg
gaggtgtcgggaaatcttcacttactattcagttctttcagaagcaatttgtcgattatt
atgatccgacgatagaagatcagtacattcaacattgcgaaatcgatggaaattgggtta
ttatggatgttctcgacacagctgggcaggaagagttctctgcaatgcgtgagcaataca
ttcgtggtgggagaggatttcttcttgtattctcagtgaccgaaagaaaatcattcgaag
aagcgcataaattgtataatcaagttcttcgagtcaaagatcgtagtgaatatccggttc
ttttggtagcaaataaggttgatttaatcaatcagagagtagtttcggagcaagagggac
gtgaattagctgctcaattgaaacttatgtacattgaaacatcggcaaaagaaccgcctg
tcaatgtggatgcggcattccatgaacttgttcgaattgttcgcagttttccaagtgatg
aaggagatcatgaggcatccatggcatctgtcccacgaaccaaaaaacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGACCGCCAGAGGATGATTCCAA 281 67 29 Antisense
GTCGGGAAATCTTCACTTACTATTC 550 471 94 Antisense
TATGGATGTTCTCGACACAGCTGGG 298 655 210 Antisense
GAGTTCTCTGCAATGCGTGAGCAAT 700 397 241 Antisense
GATTTCTTCTTGTATTCTCAGTGAC 118 429 284 Antisense
GAATATCCGGTTCTTTTGGTAGCAA 304 287 376 Antisense
AAGAACCGCCTGTCAATGTGGATGC 168 153 497 Antisense
GATGCGGCATTCCATGAACTTGTTC 187 415 517 Antisense
GAATTGTTCGCAGTTTTCCAAGTGA 384 323 542 Antisense
CATGAGGCATCCATGGCATCTGTCC 184 211 577 Antisense
CATCTGTCCCACGAACCAAAAAACG 155 213 593 Antisense
Sequence Source Affymetrix Proprietary Database