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[191468_s_at]

Description Y75B8A.2 /REP_DB=WormBase Gene ID /WP=CE23014 /GEN=nob-1 /TR=Q9XW76 /GB=CAA22093.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Homeobox domain
Cluster 4grp_high_8_8 > 13      6grp_total > 47      4grp_total > 10      6grp_cgc > 17      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 274
1 2 3 4 range
A 30
(-3)
P 		64.2
(-1.8)
P 		53
(-2.2)
P 		204.7
(3)
P 		175
B 55.3
(-2.5)
P 		55.9
(-2.5)
P 		41.7
(-3)
A 		211.3
(3)
P 		170
C 59.6
(-2.4)
P 		49.2
(-2.6)
P 		31.3
(-3)
P 		304.3
(3)
P 		273
avr 48.3
(-2.8) 		56.4
(-2.6) 		42
(-3) 		240.1
(3) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 6.1
(-2.6)
A 		15
(-1.4)
M 		39.1
(1.8)
A 		23.6
(-0.3)
A 		3.1
(-3)
A 		48.2
(3)
A 		45
B 66.4
(2.9)
M 		42.1
(0.5)
P 		35.7
(-0.1)
P 		6.2
(-3)
A 		15.8
(-2.1)
M 		67.5
(3)
P 		61
C 77.8
(3)
P 		46.7
(0.3)
P 		50.1
(0.6)
P 		9.8
(-3)
A 		37.8
(-0.5)
A 		65.3
(1.9)
A 		68
avr 50.1
(1.7) 		34.6
(-0.3) 		41.6
(0.6) 		13.2
(-3) 		18.9
(-2.3) 		60.3
(3) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 10.9
(-3)
P 		13.9
(-2.1)
P 		13.9
(-2.1)
P 		30
(3)
P 		19
B 17.6
(-1.7)
P 		13.8
(-2.3)
P 		9.7
(-3)
A 		45
(3)
P 		35
C 19.7
(-1.7)
P 		14.1
(-2.5)
P 		10.9
(-3)
P 		51.3
(3)
P 		40
avr 16.1
(-2.1) 		13.9
(-2.5) 		11.5
(-3) 		42.1
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 1.1
(-2.9)
A 		5.3
(-0.6)
M 		8.5
(1.2)
A 		6
(-0.2)
A 		0.9
(-3)
A 		11.7
(3)
A 		11
B 23.1
(2.5)
M 		14
(0.1)
P 		8.7
(-1.2)
P 		1.7
(-3)
A 		6.4
(-1.8)
M 		25.2
(3)
P 		24
C 28.8
(3)
P 		14.3
(-0.2)
P 		12.8
(-0.6)
P 		2
(-3)
A 		12.2
(-0.7)
A 		21.9
(1.5)
A 		27
avr 17.7
(2.3) 		11.2
(-0.1) 		10
(-0.5) 		3.2
(-3) 		6.5
(-1.8) 		19.6
(3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191468_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6232
Sequence Type Exemplar sequence
Representative Public ID Y75B8A.2 NCBI
Target Description Y75B8A.2 /REP_DB=WormBase Gene ID /WP=CE23014 /GEN=nob-1 /TR=Q9XW76 /GB=CAA22093.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Homeobox domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067174(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067174 NCBI Caenorhabditis elegans kNOB-like posterior (NO Backside) family member (nob-1) (nob-1) mRNA, complete cds. 11/11 None
Y75B8A.2a ENSEMBL cdna:known chromosome:CEL140:III:12078275:12084196:-1 gene:Y75B8A.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000020174 8/11 Cross Hyb Matching Probes None
SNAP00000020065 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176641 2 1 NM_001027770 175771_at (11)
NM_067174 175709_at (11), 191468_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12077974-12084134(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y75B8A.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA22093 Hypothetical protein Y75B8A.2a [Caenorhabditis elegans] ref|NP_499575.3| kNOB-like posterior (NO Backside) family member (nob-1) [Caenorhabditis elegans] gb|AAD48875.1| NOB-1B protein [Caenorhabditis elegans] 1.0E-113
blast CAC70124 Hypothetical protein Y75B8A.2b [Caenorhabditis elegans] ref|NP_001022941.1| kNOB-like posterior (NO Backside) family member (nob-1) [Caenorhabditis elegans] gb|AAD48874.1| homeodomain protein NOB-1A [Caenorhabditis elegans] 4.0E-80
blast CAE75107 Hypothetical protein CBG23030 [Caenorhabditis briggsae] 7.0E-30
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 4.00000006744953E-16
Pfam IPR001356 EMBL-EBI Homeobox 4.0E-16
Sequence
Target Sequence 
>C. ELEGANS:191468_S_AT
gctggcctcaactggttggtactcatacggacaaccgtatcaattcacagctaactactc
cattccaagtctcgatggaaatttaagtgatatcacaattccaacgacggctggaagcag
tgcggcaacaactccgaacgctgcaatgcatcttccgtgggcaatctcacatgacgggaa
gaagaagcgacaaccgtacaagaaagatcagatatcaagacttgagtacgagtattctgt
caatcaatatctcacgaataagcggagatcagaactctctgcacaattgatgcttgatga
gaagcaggtcaaggtctggttccaggtatgttataggcagtattcagcggaaagtctaga
gatcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGGCCTCAACTGGTTGGTACTCA 568 293 321 Antisense
TCAACTGGTTGGTACTCATACGGAC 449 631 328 Antisense
GTTGGTACTCATACGGACAACCGTA 413 435 335 Antisense
ACGGACAACCGTATCAATTCACAGC 441 95 347 Antisense
CAACCGTATCAATTCACAGCTAACT 454 187 352 Antisense
CAATTCACAGCTAACTACTCCATTC 135 181 361 Antisense
CAGCTAACTACTCCATTCCAAGTCT 674 203 368 Antisense
AGTCTCGATGGAAATTTAAGTGATA 146 61 388 Antisense
GTCTGGTTCCAGGTATGTTATAGGC 340 471 634 Antisense
ATGTTATAGGCAGTATTCAGCGGAA 341 47 648 Antisense
ATTCAGCGGAAAGTCTAGAGATCAT 432 11 662 Antisense
Sequence Source Affymetrix Proprietary Database