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[191458_s_at] GLP-1 protein

Description F02A9.6 /REP_DB=WormBase Gene ID /WP=CE00237 /GEN=glp-1 /TR=SW:P13508 /GB=CAA82373.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=GLP-1 protein
Cluster 6grp_total > 87      4grp_total > 24      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 179
1 2 3 4 range
A 14.7
(-3)
A 		112.2
(1.8)
A 		137.2
(3)
A 		132.2
(2.8)
A 		123
B 12.9
(-3)
A 		22.8
(-2.6)
A 		152.7
(3)
A 		104.2
(0.9)
A 		140
C 10.2
(-3)
A 		22
(-2.6)
A 		120.6
(0.7)
A 		189.3
(3)
A 		179
avr 12.6
(-3) 		52.3
(-1.2) 		136.8
(2.8) 		141.9
(3) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 823
1 2 3 4 5 6 range
A 107
(-2.7)
A 		121.7
(-2.6)
A 		71.9
(-3)
A 		202.6
(-1.9)
A 		404.8
(-0.2)
P 		777.6
(3)
P 		706
B 16.3
(-3)
A 		34.8
(-2.9)
A 		144.4
(-2.1)
A 		220
(-1.5)
P 		347
(-0.6)
P 		839.2
(3)
P 		823
C 37.9
(-3)
A 		35.6
(-3)
A 		69.1
(-2.7)
A 		333
(-0.4)
A 		445
(0.6)
P 		719.2
(3)
P 		684
avr 53.7
(-3) 		64
(-2.9) 		95.1
(-2.7) 		251.9
(-1.4) 		398.9
(-0.1) 		778.7
(3) 		725
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 5.3
(-3)
A 		24.4
(0.7)
A 		35.9
(3)
A 		19.4
(-0.2)
A 		31
B 4.1
(-3)
A 		5.6
(-2.7)
A 		35.4
(3)
A 		22.2
(0.5)
A 		31
C 3.4
(-3)
A 		6.3
(-2.5)
A 		42
(3)
A 		31.9
(1.4)
A 		39
avr 4.3
(-3) 		12.1
(-1.6) 		37.8
(3) 		24.5
(0.6) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 307
1 2 3 4 5 6 range
A 19.7
(-2.9)
A 		43.3
(-2)
A 		15.7
(-3)
A 		51.4
(-1.8)
A 		122.2
(0.7)
P 		189.3
(3)
P 		174
B 5.7
(-3)
A 		11.6
(-2.9)
A 		35.1
(-2.4)
A 		58.3
(-2)
P 		141
(-0.4)
P 		312.9
(3)
P 		307
C 14.1
(-2.9)
A 		10.8
(-3)
A 		17.6
(-2.8)
A 		67
(-1.5)
A 		144
(0.5)
P 		240.8
(3)
P 		230
avr 13.2
(-3) 		21.9
(-2.8) 		22.8
(-2.8) 		58.9
(-1.8) 		135.7
(0.1) 		247.7
(3) 		235
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191458_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6479
Sequence Type Exemplar sequence
Representative Public ID F02A9.6 NCBI
Target Description F02A9.6 /REP_DB=WormBase Gene ID /WP=CE00237 /GEN=glp-1 /TR=SW:P13508 /GB=CAA82373.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=GLP-1 protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066613(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066613 NCBI Caenorhabditis elegans abnormal Germ Line Proliferation family member (glp-1) (glp-1) mRNA, complete cds. 11/11 None
SNAP00000005915 ENSEMBL cdna:SNAP chromosome:CEL140:III:9098612:9099238:1 10/11 None
GENEFINDER00000005927 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9098636:9099238:1 9/11 None
F02A9.6 ENSEMBL cdna:known chromosome:CEL140:III:9092219:9099589:1 gene:F02A9.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9092214-9099234(+) 100.0 100.0
Public Domain and Genome References
Gene Title GLP-1 protein
Gene Symbol glp-1
Ensembl F02A9.6
Entrez Gene 176286 Entrez gene
SwissProt P13508 EMBL-EBI
WormBase CE00237 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
30154 cell differentiation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16328 lateral plasma membrane inferred from direct assay QuickGO AmiGO
12505 endomembrane system inferred from direct assay QuickGO AmiGO
5886 plasma membrane inferred from direct assay QuickGO AmiGO
5737 cytoplasm inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA82373 Hypothetical protein F02A9.6 [Caenorhabditis elegans] emb|CAA79620.1| Hypothetical protein F02A9.6 [Caenorhabditis elegans] ref|NP_499014.1| abnormal Germ Line Proliferation family member (glp-1) [Caenorhabditis elegans] sp|P13508|GLP1_CAEEL Glp-1 protein precursor gb|AAA28058.1| glp-1 protein 0.0
blast AAG49318 notch-like transmembrane receptor [Caenorhabditis remanei] 0.0
blast AAG49316 notch-like transmembrane receptor [Caenorhabditis briggsae] emb|CAE62668.1| Hypothetical protein CBG06809 [Caenorhabditis briggsae] 0.0
blast CAA82373 Hypothetical protein F02A9.6 [Caenorhabditis elegans] emb|CAA79620.1| Hypothetical protein F02A9.6 [Caenorhabditis elegans] ref|NP_499014.1| abnormal Germ Line Proliferation family member (glp-1) [Caenorhabditis elegans] sp|P13508|GLP1_CAEEL Glp-1 protein precursor gb|AAA28058.1| glp-1 protein 1.0E-92
blast CAA82373 Hypothetical protein F02A9.6 [Caenorhabditis elegans] emb|CAA79620.1| Hypothetical protein F02A9.6 [Caenorhabditis elegans] ref|NP_499014.1| abnormal Germ Line Proliferation family member (glp-1) [Caenorhabditis elegans] sp|P13508|GLP1_CAEEL Glp-1 protein precursor gb|AAA28058.1| glp-1 protein 2.0E-87
blast AAG49319 notch-like transmembrane receptor [Caenorhabditis remanei] 1.0E-29
blast AAG49318 notch-like transmembrane receptor [Caenorhabditis remanei] 1.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 5.5E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 2.5E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 3.6E-7
Pfam IPR006209 EMBL-EBI EGF-like domain 1.2E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 7.6E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 2.0E-4
Pfam IPR006209 EMBL-EBI EGF-like domain 2.1E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 6.0E-9
Pfam IPR006209 EMBL-EBI EGF-like domain 2.5E-5
Pfam IPR006209 EMBL-EBI EGF-like domain 7.3E-7
Pfam IPR002110 EMBL-EBI Ankyrin 0.45
Pfam IPR002110 EMBL-EBI Ankyrin 4.0E-5
Pfam IPR002110 EMBL-EBI Ankyrin 2.0
Pfam IPR002110 EMBL-EBI Ankyrin 2.6E-5
Pfam IPR002110 EMBL-EBI Ankyrin 2.5E-7
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-6
Pfam IPR010660 EMBL-EBI NOTCH 3.5E-60
Pfam IPR000800 EMBL-EBI Notch (DSL) domain 9.4E-21
Pfam IPR000800 EMBL-EBI Notch (DSL) domain 1.8E-20
Pfam IPR000800 EMBL-EBI Notch (DSL) domain 2.2E-21
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-6
Sequence
Target Sequence 
>C. ELEGANS:191458_S_AT
aatgctggtccgacgaagctctaataaggacaaacaggacgaagacggaagaacaccgat
aatgcttgccgctaaggaaggatgtgaaaagacggtgcaatatttggctcttaatgatgc
tagtttgggtatagtcgattcaatggacatgactgctgctcaagttgctgaagccagcta
ccatcatgaacttgcggcttttcttcgccaagtggcaaacgaacggcatcgtaatgatat
catgcgacagcaaatagtcaagtcaggtcatggtgctaagtctgggcgacaaactgtgaa
gaatatcaaaagagcaggatctcgaaaaacgccaacaagtgcagcatcgtctcgcgaaac
aaatcatctgacaccaccaccgtctgatggatcgttctcatctccgtctccacattatta
tccgacgactacatcgacaccgaatcgaatggaaacaagtccggagtacatgtttaacca
tgaaatggcaccaccggtcaatgcgatgtggtatactacaccacctccttatcaagatcc
aaactatcgtcatgtgcctccaaatactgcatttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCTGGTCCGACGAAGCTCTAAT 263 167 3288 Antisense
AGAACACCGATAATGCTTGCCGCTA 475 73 3337 Antisense
GACATGACTGCTGCTCAAGTTGCTG 362 369 3433 Antisense
GAAGCCAGCTACCATCATGAACTTG 240 345 3457 Antisense
CATGAACTTGCGGCTTTTCTTCGCC 120 211 3472 Antisense
CAACAAGTGCAGCATCGTCTCGCGA 537 189 3620 Antisense
CACCACCGTCTGATGGATCGTTCTC 223 201 3662 Antisense
TTATCCGACGACTACATCGACACCG 5 679 3705 Antisense
ACCACCTCCTTATCAAGATCCAAAC 596 83 3807 Antisense
GATCCAAACTATCGTCATGTGCCTC 653 415 3823 Antisense
ATGTGCCTCCAAATACTGCATTTCA 466 49 3839 Antisense
Sequence Source Affymetrix Proprietary Database