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[191449_s_at] Cullin family

Description ZK856.1 /REP_DB=WormBase Gene ID /WP=CE06658 /GEN=cul-5 /TR=SW:Q23639 /GB=CAA94852.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Cullin family
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 92      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 73      6grp_total > 90      4grp_total > 23      6grp_highest_sub > 04      6grp_cgc > 96      6grp_high1_sub > 04_05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 216
1 2 3 4 range
A 531.8
(2.9)
P 		373.5
(-3)
P 		379.5
(-2.8)
P 		534
(3)
P 		161
B 324.4
(-3)
P 		403
(3)
P 		351.9
(-0.9)
P 		403.3
(3)
P 		79
C 540
(3)
P 		391.6
(-2.6)
P 		499.1
(1.5)
P 		381.1
(-3)
P 		159
avr 465.4
(3) 		389.4
(-3) 		410.2
(-1.4) 		439.5
(1) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 376
1 2 3 4 5 6 range
A 422.1
(-2.9)
P 		515.2
(-1.4)
P 		416.8
(-3)
P 		792.9
(3)
P 		533.2
(-1.1)
P 		544.6
(-1)
P 		376
B 533.7
(-0.4)
P 		494.8
(-1.5)
P 		442.8
(-3)
P 		575.6
(0.8)
P 		653
(3)
P 		510.1
(-1.1)
P 		210
C 540.7
(-1)
P 		498.7
(-1.9)
P 		446.9
(-3)
P 		733.8
(3)
P 		541.9
(-1)
P 		571.5
(-0.4)
P 		287
avr 498.8
(-1.6) 		502.9
(-1.5) 		435.5
(-3) 		700.8
(3) 		576
(0.2) 		542.1
(-0.6) 		265
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 193.6
(3)
P 		81.1
(-2.8)
P 		99.2
(-1.9)
P 		78.2
(-3)
P 		115
B 103.5
(3)
P 		99.5
(1.9)
P 		81.5
(-3)
P 		85.9
(-1.8)
P 		22
C 178.8
(3)
P 		112.6
(-0.5)
P 		174
(2.7)
P 		64.3
(-3)
P 		115
avr 158.6
(3) 		97.7
(-1.4) 		118.2
(0.1) 		76.1
(-3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 77.8
(-3)
P 		183.2
(2.1)
P 		90.8
(-2.4)
P 		201.1
(3)
P 		160.9
(1)
P 		132.6
(-0.3)
P 		123
B 185.7
(-0)
P 		164.7
(-0.8)
P 		107.5
(-3)
P 		152.5
(-1.3)
P 		265.3
(3)
P 		190.2
(0.1)
P 		158
C 200.4
(3)
P 		152.2
(-0.3)
P 		113.8
(-3)
P 		147.6
(-0.7)
P 		175.4
(1.3)
P 		191.3
(2.4)
P 		87
avr 154.6
(0.1) 		166.7
(0.9) 		104
(-3) 		167.1
(0.9) 		200.5
(3) 		171.4
(1.2) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191449_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11730
Sequence Type Exemplar sequence
Representative Public ID ZK856.1 NCBI
Target Description ZK856.1 /REP_DB=WormBase Gene ID /WP=CE06658 /GEN=cul-5 /TR=SW:Q23639 /GB=CAA94852.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Cullin family
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073215(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073215 NCBI Caenorhabditis elegans CULlin family member (cul-5) (cul-5) mRNA, complete cds. 11/11 None
SNAP00000036945 ENSEMBL cdna:SNAP chromosome:CEL140:V:10182145:10185473:1 11/11 None
GENEFINDER00000036975 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10182145:10185473:1 11/11 None
ZK856.1.1 ENSEMBL cdna:known chromosome:CEL140:V:10182113:10185716:1 gene:ZK856.1 11/11 None
ZK856.1.2 ENSEMBL cdna:known chromosome:CEL140:V:10182115:10185702:1 gene:ZK856.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10182144-10185473(+) 99.87 100.0
Public Domain and Genome References
Gene Title Cullin family
Gene Symbol cul-5
Ensembl ZK856.1
Entrez Gene 179413 Entrez gene
SwissProt Q23639 EMBL-EBI
WormBase CE06658 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.19009.1.S1_S_AT similar to Cullin-5 (CUL-5) (Vasopressin-activated calcium-mobilizing receptor) (VACM-1) cfa
CANINE_2:CFAAFFX.22560.1.S1_AT similar to Cullin-5 (CUL-5) (Vasopressin-activated calcium-mobilizing receptor) (VACM-1) cfa
CANINE_2:CFAAFFX.22580.1.S1_S_AT similar to Cullin-5 (CUL-5) (Vasopressin-activated calcium-mobilizing receptor) (VACM-1) cfa
DROSOPHILA_2:1638998_AT cullin-5 dm
DROSGENOME1:150803_AT cullin-5 dm
CHICKEN:GGA.14669.1.S1_S_AT cullin 5 gga
CHICKEN:GGAAFFX.11002.1.S1_AT cullin 5 gga
HG-U133A_2:203533_S_AT cullin 5 hs
HG-U133A:203533_S_AT cullin 5 hs
HG-U133_PLUS_2:203533_S_AT cullin 5 hs
HU35KSUBA:RC_AA256943_S_AT cullin 5 hs
HU35KSUBA:AA160378_AT cullin 5 hs
HU35KSUBB:RC_AA196991_S_AT cullin 5 hs
HU35KSUBC:RC_AA403092_AT cullin 5 hs
HU35KSUBD:RC_D59972_I_AT cullin 5 hs
HUGENEFL:X81882_AT cullin 5 hs
HG-U133A:203532_X_AT cullin 5 hs
HG-U133_PLUS_2:203532_X_AT cullin 5 hs
HG-U133A_2:203532_X_AT cullin 5 hs
U133_X3P:G4503166_3P_X_AT cullin 5 hs
U133_X3P:G4503166_3P_A_AT cullin 5 hs
U133_X3P:G2394273_3P_A_AT cullin 5 hs
U133_X3P:HS.101299.0.A3_3P_AT cullin 5 hs
HG-U133A:203531_AT cullin 5 hs
HG-U133A_2:203531_AT cullin 5 hs
HG-FOCUS:203531_AT cullin 5 hs
HG-U133_PLUS_2:203531_AT cullin 5 hs
HG-U95AV2:41110_AT cullin 5 hs
HG-U95B:58347_AT cullin 5 hs
HG-U133_PLUS_2:230393_AT Cullin 5 hs
HG-U133B:230393_AT Cullin 5 hs
HG-U95C:62501_AT Cullin 5 hs
HU35KSUBA:Z21305_AT Cullin 5 hs
U133_X3P:HS.101299.1.A1_3P_AT Cullin 5 hs
MG-U74BV2:115796_AT cullin 5 mm
MG-U74BV2:113023_AT cullin 5 mm
MG-U74CV2:166816_AT cullin 5 mm
MOE430B:1452722_A_AT cullin 5 mm
MOUSE430_2:1452722_A_AT cullin 5 mm
MOE430B:1428287_AT cullin 5 mm
MOUSE430_2:1428287_AT cullin 5 mm
MOE430B:1456102_A_AT cullin 5 mm
MOUSE430_2:1456102_A_AT cullin 5 mm
MG-U74CV2:129582_AT Cullin 5, mRNA (cDNA clone IMAGE:4947730) mm
MG-U74CV2:165502_AT Cullin 5, mRNA (cDNA clone IMAGE:4947730) mm
MU19KSUBC:TC38063_AT Cullin 5, mRNA (cDNA clone IMAGE:4947730) mm
MU19KSUBC:TC42001_AT Cullin 5, mRNA (cDNA clone IMAGE:4947730) mm
RAE230A:1369068_AT cullin 5 rn
RAT230_2:1369068_AT cullin 5 rn
RAE230B:1393306_AT Cullin 5 rn
RAT230_2:1393306_AT Cullin 5 rn
RAE230B:1393930_AT Cullin 5 rn
RAT230_2:1393930_AT Cullin 5 rn
RAE230B:1396018_AT Cullin 5 rn
RAT230_2:1396018_AT Cullin 5 rn
RG-U34B:RC_AI029426_AT Cullin 5 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94852 Hypothetical protein ZK856.1 [Caenorhabditis elegans] ref|NP_505616.1| CULlin family member (cul-5) [Caenorhabditis elegans] sp|Q23639|CUL5_CAEEL Cullin-5 0.0
blast CAE64749 Hypothetical protein CBG09538 [Caenorhabditis briggsae] 0.0
blast XP_683787 PREDICTED: similar to Cullin-5 (CUL-5) (Vasopressin-activated calcium-mobilizing receptor) (VACM-1) [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.SCF ubiquitin ligase complex WHB domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; SCF ubiquitin ligase complex WHB domain 1.0000000116861E-7
Pfam IPR001373 EMBL-EBI Cullin 1.0E-126
Pfam IPR001373 EMBL-EBI Cullin 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191449_S_AT
ggtcttcggaactgcaaatggcggcagattcgatcttgaatgcacaacgttccagatggc
agttctattttgtttcaatgatagagctcatgataaaatatcattggaaactctacggct
tgctactgaattaccggatgctgaactgaatcgcacacttctgtcactggttgcatatcc
aaagatgagataccaaattcttctttgcgacgttccttcgacaaccgtcacagccagaga
tttcactgattctacaaaattccttattaatcatgacttcaatgttgtgaaaaatggaaa
gtcacagcaacgtggaaaagttaatttgataggaagacttcagttgtcattggaagccaa
cgctgagaaggaacacgagagcatcgtagcattgcgggagctccgcgttcaagaaggaat
tgtgaagattttgaaaactagaaaaacttacacattggctcagctcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCTTCGGAACTGCAAATGGCGGC 418 503 1632 Antisense
GAATGCACAACGTTCCAGATGGCAG 182 331 1669 Antisense
GATGGCAGTTCTATTTTGTTTCAAT 361 407 1686 Antisense
TCATTGGAAACTCTACGGCTTGCTA 132 575 1732 Antisense
GGCTTGCTACTGAATTACCGGATGC 270 537 1748 Antisense
GATGCTGAACTGAATCGCACACTTC 694 411 1768 Antisense
CAAATTCTTCTTTGCGACGTTCCTT 405 193 1825 Antisense
AACCGTCACAGCCAGAGATTTCACT 73 147 1854 Antisense
AGCATCGTAGCATTGCGGGAGCTCC 157 79 2011 Antisense
GGGAGCTCCGCGTTCAAGAAGGAAT 603 495 2027 Antisense
AAACTTACACATTGGCTCAGCTCAC 372 127 2075 Antisense
Sequence Source Affymetrix Proprietary Database