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[191447_at]

Description T21F2.1 /REP_DB=WormBase Gene ID /WP=CE07524 /TR=Q22658 /GB=AAA83590.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ligand-gated ionic channel
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 06      4grp_high_8_8 > 10      6grp_highest_nonorm > 90      6grp_total > 07      4grp_total > 14      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 149
(3)
P 		76.2
(-2.6)
P 		71.5
(-3)
P 		135.7
(2)
P 		78
B 105
(1.3)
P 		56.5
(-3)
P 		62.5
(-2.5)
P 		124.3
(3)
P 		68
C 112.5
(1.1)
P 		57.3
(-2.1)
A 		41.7
(-3)
P 		145.6
(3)
P 		104
avr 122.2
(2) 		63.3
(-2.6) 		58.6
(-3) 		135.2
(3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 115
(3)
P 		68.8
(-2.6)
P 		109.9
(2.4)
P 		94.4
(0.5)
P 		65.8
(-3)
P 		73.4
(-2.1)
P 		49
B 121
(3)
P 		87.6
(-0.1)
P 		55.4
(-3)
P 		75.6
(-1.2)
P 		80
(-0.8)
P 		75.5
(-1.2)
P 		66
C 93.1
(2)
P 		100.9
(3)
P 		69.9
(-0.8)
P 		52
(-3)
P 		65.7
(-1.3)
P 		59
(-2.1)
P 		49
avr 109.7
(3) 		85.8
(-0.5) 		78.4
(-1.6) 		74
(-2.3) 		70.5
(-2.8) 		69.3
(-3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 54.2
(3)
P 		16.5
(-3)
P 		18.7
(-2.6)
P 		19.9
(-2.5)
P 		38
B 33.5
(3)
P 		13.9
(-3)
P 		14.5
(-2.8)
P 		26.5
(0.9)
P 		20
C 37.3
(3)
P 		16.5
(-2.5)
A 		14.5
(-3)
P 		24.5
(-0.4)
P 		23
avr 41.7
(3) 		15.6
(-3) 		15.9
(-2.9) 		23.6
(-1.2) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 21.2
(0)
P 		24.5
(3)
P 		23.9
(2.5)
P 		23.9
(2.5)
P 		19.8
(-1.3)
P 		17.9
(-3)
P 		7
B 42.1
(3)
P 		29.2
(0.3)
P 		13.4
(-3)
P 		20
(-1.6)
P 		32.5
(1)
P 		28.2
(0.1)
P 		29
C 34.5
(3)
P 		30.8
(2.1)
P 		17.8
(-1.2)
P 		10.5
(-3)
P 		21.3
(-0.3)
P 		19.8
(-0.7)
P 		24
avr 32.6
(3) 		28.2
(1.2) 		18.4
(-2.9) 		18.1
(-3) 		24.5
(-0.4) 		22
(-1.4) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191447_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17485
Sequence Type Exemplar sequence
Representative Public ID T21F2.1 NCBI
Target Description T21F2.1 /REP_DB=WormBase Gene ID /WP=CE07524 /TR=Q22658 /GB=AAA83590.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ligand-gated ionic channel
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171812(11), NM_171813(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171812 NCBI Caenorhabditis elegans T21F2.1a (T21F2.1) mRNA, complete cds. 11/11 None
NM_171813 NCBI Caenorhabditis elegans T21F2.1b (T21F2.1) mRNA, complete cds. 11/11 None
GENEFINDER00000037511 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16710749:16720386:1 11/11 None
T21F2.1a ENSEMBL cdna:known chromosome:CEL140:X:16711594:16720571:1 gene:T21F2.1 11/11 None
T21F2.1b ENSEMBL cdna:known chromosome:CEL140:X:16718456:16720386:1 gene:T21F2.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000037505 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16717709-16720386(+) 97.99 100.0
Public Domain and Genome References
Ensembl T21F2.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM69066 Hypothetical protein T21F2.1a [Caenorhabditis elegans] ref|NP_741945.2| T21F2.1a [Caenorhabditis elegans] 0.0
blast T30013 hypothetical protein T21F2.1 - Caenorhabditis elegans 0.0
blast CAE69946 Hypothetical protein CBG16329 [Caenorhabditis briggsae] 0.0
blast AAM69067 Hypothetical protein T21F2.1b [Caenorhabditis elegans] ref|NP_741946.1| T21F2.1b [Caenorhabditis elegans] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 9.6E-20
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.9E-13
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 2.2E-28
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 9.6E-20
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 2.0E-10
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 9.6E-20
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 7.0E-53
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 9.6E-20
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.9E-13
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.1E-33
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741945.2 4 303-325,332-354,369-391,479-501
NP_741946.1 4 84-106,113-135,150-172,260-282
T21F2.1a 4 295-317,324-346,361-383,471-493
T21F2.1b 4 84-106,113-135,150-172,260-282
GENEFINDER00000037511 4 358-380,387-409,424-446,534-556
Sequence
Target Sequence 
>C. ELEGANS:191447_AT
gtgcttcaggcgtatatgccgacatatctatcggttttcatatcatggattgctttttgg
attgacaccaaggcgcttcctgctcgaataacccttggcgtatcttctctcatggctctt
actttccaatttggtaatattgtgaaaaacctaccacgtgtcagttacgtgaaagctctc
gatatctggatgttcggatgcgtgggcttcattttcttatccctagtcgagctcgctgtt
gtaggatttgctgacaaattggaggcgaaacggagaagacataatagatgcaaagagcaa
ttgatgatgagaagcgattcagagcagcaatggctttcaaggttgtccgggcaacgacct
caagtctcagaaaccaattcagatgctacccacacgattcaaattaacgatgggaatggg
aacgtgaggcggagaaaaagtgaagaaaaacggcagaaacttctgctccaaatggaaagc
cctatgtttgttaatggggaaaaaattgacgagatctcagcaaagctgtttccactttta
ttcacggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCTTCAGGCGTATATGCCGACAT 127 479 607 Antisense
TCGAATAACCCTTGGCGTATCTTCT 264 595 690 Antisense
TCTCATGGCTCTTACTTTCCAATTT 475 611 714 Antisense
GAAAGCTCTCGATATCTGGATGTTC 619 359 777 Antisense
TGGATGTTCGGATGCGTGGGCTTCA 656 549 793 Antisense
TTCATTTTCTTATCCCTAGTCGAGC 550 683 814 Antisense
GTCGAGCTCGCTGTTGTAGGATTTG 13 475 832 Antisense
GCAATGGCTTTCAAGGTTGTCCGGG 693 319 933 Antisense
GGCAACGACCTCAAGTCTCAGAAAC 230 535 956 Antisense
AGATGCTACCCACACGATTCAAATT 260 67 987 Antisense
AGCTGTTTCCACTTTTATTCACGGC 582 81 1130 Antisense
Sequence Source Affymetrix Proprietary Database