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[191430_at] glutathione S-transferase

Description Y53F4B.37 /REP_DB=WormBase Gene ID /WP=CE26161 /TR=Q9NA96 /GB=CAB97240.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster 6grp_total > 07      4grp_total > 37      6grp_cgc > 24      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 37.6
(3)
A 		6.9
(-3)
A 		11.9
(-2)
A 		12.2
(-2)
A 		31
B 3.7
(-2.7)
A 		4.4
(-2.5)
A 		2.8
(-3)
A 		22.3
(3)
A 		20
C 20.7
(1)
A 		30.3
(3)
A 		1.5
(-3)
A 		3.3
(-2.6)
A 		29
avr 20.7
(3) 		13.9
(0.3) 		5.4
(-3) 		12.6
(-0.2) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 71.5
(3)
A 		49.4
(0.9)
A 		68.6
(2.7)
A 		30.6
(-0.8)
A 		7.6
(-3)
A 		35.1
(-0.4)
A 		64
B 97.6
(3)
P 		82.4
(2)
A 		60.2
(0.4)
A 		24.3
(-2)
A 		10.5
(-3)
A 		27.7
(-1.8)
A 		87
C 107.3
(3)
P 		49.9
(-1.3)
A 		47.9
(-1.4)
A 		44.8
(-1.7)
A 		26.8
(-3)
A 		47.1
(-1.5)
A 		81
avr 92.1
(3) 		60.6
(0.5) 		58.9
(0.4) 		33.2
(-1.6) 		15
(-3) 		36.6
(-1.3) 		77
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 13.7
(3)
A 		1.5
(-3)
A 		3.1
(-2.2)
A 		1.8
(-2.9)
A 		12
B 1.2
(-2.1)
A 		1.1
(-2.3)
A 		0.6
(-3)
A 		4.8
(3)
A 		4
C 6.9
(1.7)
A 		8.7
(3)
A 		0.5
(-3)
A 		0.5
(-3)
A 		8
avr 7.3
(3) 		3.8
(-0.6) 		1.4
(-3) 		2.4
(-2) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 13.2
(1.3)
A 		17.6
(3)
A 		14.9
(1.9)
A 		7.7
(-0.9)
A 		2.3
(-3)
A 		8.5
(-0.6)
A 		15
B 33.9
(3)
P 		27.4
(1.7)
A 		14.6
(-0.9)
A 		6.4
(-2.6)
A 		4.3
(-3)
A 		10.3
(-1.8)
A 		30
C 39.8
(3)
P 		15.2
(-1.7)
A 		12.2
(-2.3)
A 		9
(-2.9)
A 		8.7
(-3)
A 		15.8
(-1.6)
A 		31
avr 29
(3) 		20.1
(0.8) 		13.9
(-0.8) 		7.7
(-2.3) 		5.1
(-3) 		11.5
(-1.4) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191430_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3654
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.37 NCBI
Target Description Y53F4B.37 /REP_DB=WormBase Gene ID /WP=CE26161 /TR=Q9NA96 /GB=CAB97240.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064722(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064722 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-32) (gst-32) mRNA, complete cds. 11/11 None
Y53F4B.37 ENSEMBL cdna:known chromosome:CEL140:II:15177262:15178475:-1 gene:Y53F4B.37 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15177258-15177744(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-32
Ensembl Y53F4B.37
Entrez Gene 190230 Entrez gene
SwissProt Q9NA96 EMBL-EBI
WormBase CE32271 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB97240 Hypothetical protein Y53F4B.37 [Caenorhabditis elegans] ref|NP_497123.2| Glutathione S-Transferase family member (gst-32) [Caenorhabditis elegans] 1.0E-119
blast CAE73239 Hypothetical protein CBG20648 [Caenorhabditis briggsae] 3.0E-91
blast CAB61107 Hypothetical protein Y53F4B.33 [Caenorhabditis elegans] ref|NP_497119.1| Glutathione S-Transferase family member (gst-39) [Caenorhabditis elegans] 4.0E-90
Protein Domains
Database ID Description E-Value
scop a.4.5.Transcription factor MotA, activation domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Transcription factor MotA, activation domain 0.649999976158142
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 5.9E-9
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 4.6E-17
Sequence
Target Sequence 
>C. ELEGANS:191430_AT
ggcttgggcagatgcgatttgtgatcaagtgaaggacttcatcgattctttcaaacaaat
tgtgatagcaaaacgtgatgggaagacggctgaggaaattgaaaaaatccataccgagat
tttcctcccagcaaaagattcgtacttcaagattattaatggtatccttgagaagagcaa
gtcaggatttctagttggcgatagtttaactttcgccgatattgttgtcgtggaaaactt
caccacattagacaaaaaccattattgcaacgcttcggagcaaccaagattgacggctct
cagggagaaggtctacgcgattccagcaattaagcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTTGGGCAGATGCGATTTGTGAT 481 537 129 Antisense
ACTTCATCGATTCTTTCAAACAAAT 120 103 164 Antisense
GTGATGGGAAGACGGCTGAGGAAAT 515 483 203 Antisense
GAGATTTTCCTCCCAGCAAAAGATT 550 395 244 Antisense
AAGTCAGGATTTCTAGTTGGCGATA 704 157 307 Antisense
CGATAGTTTAACTTTCGCCGATATT 660 247 327 Antisense
GGAAAACTTCACCACATTAGACAAA 31 529 360 Antisense
AACCATTATTGCAACGCTTCGGAGC 151 147 385 Antisense
GCAACCAAGATTGACGGCTCTCAGG 288 317 408 Antisense
TGACGGCTCTCAGGGAGAAGGTCTA 266 571 419 Antisense
CTACGCGATTCCAGCAATTAAGCAT 616 223 441 Antisense
Sequence Source Affymetrix Proprietary Database