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[191419_at]

Description C24A8.4 /REP_DB=WormBase Gene ID /WP=CE08340 /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor (ST.LOUIS) TR:P91098 protein_id:AAB37981.1
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      6grp_highest_nonorm > 80      6grp_total > 26      4grp_total > 22      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 154.7
(2.5)
P 		63.6
(-1.7)
P 		34.7
(-3)
A 		165.5
(3)
P 		131
B 186.4
(3)
P 		78.2
(-2)
P 		55.5
(-3)
M 		182.1
(2.8)
P 		131
C 207.9
(1.9)
P 		80.2
(-2.7)
P 		72.6
(-3)
A 		238.7
(3)
P 		166
avr 183
(2.5) 		74
(-2.2) 		54.3
(-3) 		195.4
(3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 258
1 2 3 4 5 6 range
A 203.7
(1.1)
P 		253.2
(3)
P 		164.1
(-0.4)
P 		94.7
(-3)
P 		99.1
(-2.8)
P 		144
(-1.1)
P 		159
B 293.1
(3)
P 		281.6
(2.7)
P 		150.5
(-1.1)
P 		102.3
(-2.5)
P 		84.8
(-3)
P 		115.2
(-2.1)
P 		208
C 330
(3)
P 		202.4
(0)
P 		115.3
(-2)
P 		112.2
(-2.1)
P 		72.4
(-3)
P 		117.6
(-1.9)
P 		258
avr 275.6
(3) 		245.7
(2.1) 		143.3
(-1.2) 		103.1
(-2.4) 		85.4
(-3) 		125.6
(-1.7) 		190
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 56.3
(3)
P 		13.8
(-2.4)
P 		9.1
(-3)
A 		24.2
(-1.1)
P 		47
B 59.4
(3)
P 		19.3
(-2.2)
P 		12.9
(-3)
M 		38.8
(0.3)
P 		47
C 68.8
(3)
P 		23
(-3)
P 		25.3
(-2.7)
A 		40.3
(-0.7)
P 		46
avr 61.5
(3) 		18.7
(-2.6) 		15.8
(-3) 		34.4
(-0.6) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 37.5
(-1.8)
P 		90.1
(3)
P 		35.7
(-1.9)
P 		24
(-3)
P 		29.9
(-2.5)
P 		35.1
(-2)
P 		66
B 102
(3)
P 		93.7
(2.3)
P 		36.6
(-2.2)
P 		27.1
(-3)
P 		34.5
(-2.4)
P 		42.9
(-1.7)
P 		75
C 122.3
(3)
P 		61.8
(-0.6)
P 		29.4
(-2.6)
P 		22.6
(-3)
P 		23.4
(-3)
P 		39.4
(-2)
P 		100
avr 87.3
(3) 		81.9
(2.5) 		33.9
(-2.1) 		24.6
(-3) 		29.3
(-2.6) 		39.1
(-1.6) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191419_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16346
Sequence Type Exemplar sequence
Representative Public ID C24A8.4 NCBI
Target Description C24A8.4 /REP_DB=WormBase Gene ID /WP=CE08340 /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor (ST.LOUIS) TR:P91098 protein_id:AAB37981.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076340(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076340 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-306) (tag-306) mRNA, complete cds. 11/11 A 
GENEFINDER00000036350 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4320837:4327305:1 11/11 None
SNAP00000036344 ENSEMBL cdna:SNAP chromosome:CEL140:X:4326633:4327305:1 9/11 None
SNAP00000036346 ENSEMBL cdna:SNAP chromosome:CEL140:X:4330052:4330724:-1 9/11 None
C24A8.4a ENSEMBL cdna:known chromosome:CEL140:X:4330052:4336008:-1 gene:C24A8.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_076338 1/11 Cross Hyb Matching Probes A 
C24A8.1 1/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180707 2 1 NM_076340 191377_s_at (11), 191419_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4324152-4327306(+) 66.76 66.76
chrX:4330052-4333815(-) 100.0 100.0
Public Domain and Genome References
Ensembl C24A8.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39183 Temporarily assigned gene name protein 306, isoform a [Caenorhabditis elegans] ref|NP_508741.2| Temporarily Assigned Gene name family member (tag-306) [Caenorhabditis elegans] 0.0
blast AAK39182 Temporarily assigned gene name protein 306, isoform b [Caenorhabditis elegans] ref|NP_508742.1| Temporarily Assigned Gene name family member (tag-306) [Caenorhabditis elegans] 0.0
blast AAK68327 Hypothetical protein F14H12.4b [Caenorhabditis elegans] ref|NP_001024575.1| F14H12.4b [Caenorhabditis elegans] 0.0
blast CAE68558 Hypothetical protein CBG14393 [Caenorhabditis briggsae] 0.0
blast AAK39179 Hypothetical protein C24A8.1 [Caenorhabditis elegans] ref|NP_508739.1| C24A8.1 [Caenorhabditis elegans] 1.0E-139
blast AAK39183 Temporarily assigned gene name protein 306, isoform a [Caenorhabditis elegans] ref|NP_508741.2| Temporarily Assigned Gene name family member (tag-306) [Caenorhabditis elegans] 1.0E-108
blast T25567 hypothetical protein C24A8.4 - Caenorhabditis elegans 6.0E-38
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-65
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-65
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 2.0E-71
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 1.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 8.5E-93
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.2E-19
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.1E-17
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.0E-63
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508741.2 2 494-516,531-553
C24A8.4a 2 447-469,484-506
GENEFINDER00000036350 6 26-48,63-85,106-128,155-174,485-507,522-544
Sequence
Target Sequence 
>C. ELEGANS:191419_AT
aatcaatccaaaattccgtcattcattccgccgcttacttggcttccgtcgatcttctcg
acgtcagagagaaaaatcgtggatgttaccgccacgagatagcagcagaccaatgaacaa
cagaaaaatttccatgcaaaaaccagaaatcgtcattgacgaaatttcaccgaatcgaat
ttcaatctccgccggaggaaacacaacttttaggcgatcttcggatgattgtattcgtag
atcatcttctgaaataatcggcggtatcactcttccatctcaaagtagtaatgataaacg
aagaggatccggtatgtcaaatagttcagcatctctccatatccatgaaacgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCAATCCAAAATTCCGTCATTCA 330 171 639 Antisense
GATAGCAGCAGACCAATGAACAACA 376 423 736 Antisense
GTCATTGACGAAATTTCACCGAATC 652 467 790 Antisense
ACCGAATCGAATTTCAATCTCCGCC 334 89 807 Antisense
ATCTCCGCCGGAGGAAACACAACTT 425 33 823 Antisense
AACACAACTTTTAGGCGATCTTCGG 533 133 838 Antisense
AAATAATCGGCGGTATCACTCTTCC 140 117 890 Antisense
GTATCACTCTTCCATCTCAAAGTAG 42 455 902 Antisense
GAAGAGGATCCGGTATGTCAAATAG 144 339 938 Antisense
GTCAAATAGTTCAGCATCTCTCCAT 165 465 954 Antisense
GCATCTCTCCATATCCATGAAACGC 645 307 967 Antisense
Sequence Source Affymetrix Proprietary Database