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[191414_s_at] glutathione S-transferase

Description C02A12.1 /REP_DB=WormBase Gene ID /WP=CE07825 /TR=O16198 /GB=AAB65264.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=glutathione S-transferase
Cluster 6grp_total > 18      4grp_total > 21      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 117.4
(1.9)
A 		69.1
(-0.5)
A 		20.9
(-3)
A 		137.9
(3)
A 		117
B 87.3
(0.5)
A 		91.8
(0.8)
A 		37.5
(-3)
A 		123.4
(3)
A 		86
C 83.6
(-0.7)
A 		70.1
(-1.7)
A 		52.7
(-3)
A 		131.8
(3)
A 		79
avr 96.1
(0.8) 		77
(-0.4) 		37
(-3) 		131
(3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 592
1 2 3 4 5 6 range
A 603
(3)
P 		167.6
(-2)
A 		118.6
(-2.6)
A 		84.4
(-3)
A 		91
(-2.9)
A 		173
(-2)
A 		519
B 591.7
(3)
P 		142.1
(-1.7)
A 		98.6
(-2.1)
A 		12.4
(-3)
A 		124.8
(-1.8)
A 		152.1
(-1.6)
M 		579
C 604.3
(3)
P 		119.9
(-1.9)
A 		140.8
(-1.7)
A 		16.4
(-3)
A 		82.6
(-2.3)
A 		196
(-1.2)
A 		588
avr 599.7
(3) 		143.2
(-1.9) 		119.3
(-2.1) 		37.7
(-3) 		99.5
(-2.3) 		173.7
(-1.5) 		562
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 42.7
(3)
A 		15
(-1.5)
A 		5.5
(-3)
A 		20.2
(-0.6)
A 		37
B 27.8
(3)
A 		22.7
(1.4)
A 		8.7
(-3)
A 		26.3
(2.5)
A 		19
C 27.7
(3)
A 		20.2
(-1.8)
A 		18.4
(-3)
A 		22.2
(-0.5)
A 		9
avr 32.7
(3) 		19.3
(-0.7) 		10.9
(-3) 		22.9
(0.3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 221
1 2 3 4 5 6 range
A 111.2
(3)
P 		59.6
(-0.4)
A 		25.8
(-2.7)
A 		21.4
(-3)
A 		27.5
(-2.6)
A 		42.1
(-1.6)
A 		90
B 205.9
(3)
P 		47.3
(-1.7)
A 		24
(-2.4)
A 		3.3
(-3)
A 		50.7
(-1.6)
A 		56.7
(-1.4)
M 		203
C 224
(3)
P 		36.6
(-2.1)
A 		35.8
(-2.1)
A 		3.3
(-3)
A 		26.7
(-2.4)
A 		65.6
(-1.3)
A 		221
avr 180.4
(3) 		47.8
(-1.6) 		28.5
(-2.3) 		9.3
(-3) 		35
(-2.1) 		54.8
(-1.4) 		171
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191414_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14846
Sequence Type Exemplar sequence
Representative Public ID C02A12.1 NCBI
Target Description C02A12.1 /REP_DB=WormBase Gene ID /WP=CE07825 /TR=O16198 /GB=AAB65264.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=glutathione S-transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071567(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071567 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-33) (gst-33) mRNA, complete cds. 11/11 None
SNAP00000023215 ENSEMBL cdna:SNAP chromosome:CEL140:V:3467359:3469438:-1 11/11 None
GENEFINDER00000023222 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3467359:3468315:-1 11/11 None
C02A12.1 ENSEMBL cdna:known chromosome:CEL140:V:3467348:3468327:-1 gene:C02A12.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3467358-3468315(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-33
Ensembl C02A12.1
Entrez Gene 178771 Entrez gene
SwissProt O16198 EMBL-EBI
WormBase CE07825 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65264 Glutathione s-transferase protein 33 [Caenorhabditis elegans] ref|NP_503968.1| Glutathione S-Transferase family member (gst-33) [Caenorhabditis elegans] 1.0E-114
blast AAB65264 Glutathione s-transferase protein 33 [Caenorhabditis elegans] ref|NP_503968.1| Glutathione S-Transferase family member (gst-33) [Caenorhabditis elegans] 1.0E-113
blast CAE74620 Hypothetical protein CBG22411 [Caenorhabditis briggsae] 7.0E-95
blast CAE74620 Hypothetical protein CBG22411 [Caenorhabditis briggsae] 9.0E-95
blast AAF81283 glutathione S-transferase [Haemonchus contortus] 3.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 4.3E-9
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 1.7E-20
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 4.3E-9
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 1.7E-20
Sequence
Target Sequence 
>C. ELEGANS:191414_S_AT
tcactacttcaacgctcgcggatatgcagaggcttcgagagctatgttccatatggccgg
cgtcgaattcgaagatgtccgatacgaaatcgatgactggatcaaggaagaaaacaccct
taaaaacgagatgccgttcgggcaaatgcctgtgctagaagtcgacggtgagaagatccc
acaatccgttgcaatcgctcgctttgttgccaatcaacttggatttgctggacaaactcc
ggtagagaaggcctgggctgacgcattcaccgatctttacaaggacttcttgattgacat
gaagccatgggcaatgattgcctttgggtatcctggtgctgctggggatcgcgacgagtt
gaagaaaacttcgttggaccctgctaaggagaagtatttcaagcttctgagcaagagatt
ggagaagagcaaatctggattcctcctggacagtggaatctcgtttcccgatctcttctt
cttcgaaaccacaacttctttgattgagttggaaaaaggatttttgggaaccgatttccc
agtagtcaatgcctacttcaagcgtatcgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCACTACTTCAACGCTCGCGGATAT 25 625 30 Antisense
TCCATATGGCCGGCGTCGAATTCGA 18 587 77 Antisense
ATGCCTGTGCTAGAAGTCGACGGTG 703 39 175 Antisense
TGAGAAGATCCCACAATCCGTTGCA 388 569 198 Antisense
TTGCAATCGCTCGCTTTGTTGCCAA 129 705 218 Antisense
AGAGAAGGCCTGGGCTGACGCATTC 401 69 273 Antisense
GACGCATTCACCGATCTTTACAAGG 150 381 289 Antisense
AATGATTGCCTTTGGGTATCCTGGT 31 163 342 Antisense
CAAATCTGGATTCCTCCTGGACAGT 13 193 459 Antisense
GACAGTGGAATCTCGTTTCCCGATC 125 369 478 Antisense
CAATGCCTACTTCAAGCGTATCGCT 144 179 576 Antisense
Sequence Source Affymetrix Proprietary Database