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[191405_at]

Description C50F7.1 /REP_DB=WormBase Gene ID /WP=CE04239 /TR=Q18757 /GB=AAA83308.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 15      4grp_high_8_8 > 10      6grp_highest_nonorm > 90      6grp_total > 04      4grp_total > 22      6grp_highest_sub > De      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 139.5
(3)
P 		97.7
(-1.1)
P 		78.9
(-3)
P 		126.3
(1.7)
P 		61
B 138.8
(3)
P 		83.2
(-1.8)
P 		68.6
(-3)
P 		107.4
(0.3)
P 		70
C 161.7
(1.6)
P 		75.4
(-1.7)
P 		40.7
(-3)
M 		200.2
(3)
P 		160
avr 146.7
(3) 		85.4
(-1.4) 		62.7
(-3) 		144.6
(2.9) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 132.6
(3)
P 		107.9
(0.2)
P 		104.9
(-0.2)
P 		87
(-2.3)
P 		80.6
(-3)
P 		86
(-2.4)
P 		52
B 133.9
(2.5)
P 		139.8
(3)
P 		85.1
(-1.5)
P 		67.3
(-3)
P 		118.4
(1.2)
P 		86.6
(-1.4)
P 		73
C 168.7
(3)
P 		143.9
(1.6)
P 		108.6
(-0.5)
P 		154.6
(2.2)
P 		81.8
(-2)
P 		65.3
(-3)
P 		103
avr 145.1
(3) 		130.5
(1.7) 		99.5
(-1.2) 		103
(-0.8) 		93.6
(-1.7) 		79.3
(-3) 		66
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 50.8
(3)
P 		21.2
(-2.5)
P 		20.6
(-2.6)
P 		18.5
(-3)
P 		32
B 44.3
(3)
P 		20.6
(-2)
P 		15.9
(-3)
P 		22.9
(-1.5)
P 		28
C 53.5
(3)
P 		21.7
(-1.9)
P 		14.2
(-3)
M 		33.7
(-0)
P 		39
avr 49.5
(3) 		21.2
(-2.2) 		16.9
(-3) 		25
(-1.5) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 24.4
(-1.8)
P 		38.4
(3)
P 		22.8
(-2.3)
P 		22.1
(-2.6)
P 		24.3
(-1.8)
P 		20.9
(-3)
P 		18
B 46.6
(2.7)
P 		46.5
(2.7)
P 		20.7
(-2.4)
P 		17.8
(-3)
P 		48.1
(3)
P 		32.3
(-0.1)
P 		30
C 62.5
(3)
P 		43.9
(0.3)
P 		27.6
(-2.2)
P 		31.1
(-1.6)
P 		26.5
(-2.3)
P 		21.9
(-3)
P 		41
avr 44.5
(3) 		42.9
(2.5) 		23.7
(-3) 		23.7
(-3) 		33
(-0.3) 		25
(-2.6) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191405_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10006
Sequence Type Exemplar sequence
Representative Public ID C50F7.1 NCBI
Target Description C50F7.1 /REP_DB=WormBase Gene ID /WP=CE04239 /TR=Q18757 /GB=AAA83308.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027917(11), NM_001027916(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027917 NCBI Caenorhabditis elegans C50F7.1b (C50F7.1) mRNA, complete cds. 11/11 None
NM_001027916 NCBI Caenorhabditis elegans C50F7.1a (C50F7.1) mRNA, complete cds. 11/11 None
SNAP00000024191 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7743889:7745699:1 11/11 None
GENEFINDER00000024197 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7743889:7745833:1 11/11 None
C50F7.1b ENSEMBL cdna:known chromosome:CEL140:IV:7743889:7746057:1 gene:C50F7.1 11/11 None
C50F7.1a ENSEMBL cdna:known chromosome:CEL140:IV:7744659:7745699:1 gene:C50F7.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7743894-7745705(+) 100.0 100.0
Public Domain and Genome References
Ensembl C50F7.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAQ01534 Hypothetical protein C50F7.1b [Caenorhabditis elegans] ref|NP_001023088.1| C50F7.1b [Caenorhabditis elegans] 0.0
blast CAE70869 Hypothetical protein CBG17657 [Caenorhabditis briggsae] 0.0
blast AAA83308 Hypothetical protein C50F7.1a [Caenorhabditis elegans] ref|NP_001023087.1| C50F7.1a [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.4E-55
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.1E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023088.1 7 32-54,66-88,108-130,150-172,192-214,246-268,283-305
NP_001023087.1 3 39-61,94-116,131-153
C50F7.1b 7 32-54,66-88,108-130,150-172,192-214,246-268,283-305
C50F7.1a 3 39-61,94-116,131-153
SNAP00000024191 7 32-54,66-88,108-130,150-172,192-214,246-268,283-305
GENEFINDER00000024197 7 32-54,66-88,108-130,150-172,192-214,246-268,283-305
Sequence
Target Sequence 
>C. ELEGANS:191405_AT
atggttaccgattatgtacgtcacaaacggatcaaaaattatattttttatttgttaact
tattgctggctttcatagttccatttggtttgatttcgggtctatacacgagaatattta
tcactgtatcaacacatagaagtctggcagttgatgcaagagcccgagaagatcgagtaa
aactacgagttgccacaatgatgttaacagtaataattgtatttgcttgttgctggttac
ctctctattgcatcttcacatattttttcttctttgctgatcagcgatcggatctttttc
aaattacttcaatgttgattcggccaatattccaatggatgtcattactatcgagttctt
taaatccaataatttatattgcatacagtcacaagtacagaagagctttcaaaagtatat
tactgatgccatgtaagacaagatacgaaagagtccgaagcacaatactccgtcgtcact
ctcgtggcttcaaatcaactgcaacaatttcaatgtctaattttggaactgaaccaacaa
atttaggtggagcatctagtttacttatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGTTACCGATTATGTACGTCACA 343 49 545 Antisense
ACTTATTGCTGGCTTTCATAGTTCC 77 103 602 Antisense
TTGGTTTGATTTCGGGTCTATACAC 603 707 629 Antisense
GTATTTGCTTGTTGCTGGTTACCTC 8 453 763 Antisense
TCTCTATTGCATCTTCACATATTTT 281 613 786 Antisense
ATTTTTTCTTCTTTGCTGATCAGCG 424 15 806 Antisense
ATGTTGATTCGGCCAATATTCCAAT 222 47 856 Antisense
GGATGTCATTACTATCGAGTTCTTT 425 513 881 Antisense
GAGTCCGAAGCACAATACTCCGTCG 478 395 995 Antisense
CACTCTCGTGGCTTCAAATCAACTG 76 199 1021 Antisense
AGGTGGAGCATCTAGTTTACTTATT 318 57 1089 Antisense
Sequence Source Affymetrix Proprietary Database