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[191404_at]

Description F43H9.2 /REP_DB=WormBase Gene ID /WP=CE07246 /CHR=5 /FEA=Sanger Annotation /DEF=serine palmtoyltransferase (ST.LOUIS) TR:Q20375 protein_id:AAA92303.1
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 41      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 23      6grp_total > 31      4grp_total > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1748
1 2 3 4 range
A 2043.8
(2.7)
P 		1539.9
(1)
P 		2124.9
(3)
P 		400.3
(-3)
P 		1725
B 1980.4
(2.6)
P 		1690.5
(1.6)
P 		2103.6
(3)
P 		376.7
(-3)
P 		1727
C 1854
(2.5)
P 		1569.3
(1.4)
P 		1981.1
(3)
P 		398.5
(-3)
P 		1583
avr 1959.4
(2.6) 		1599.9
(1.3) 		2069.9
(3) 		391.8
(-3) 		1678

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2742
1 2 3 4 5 6 range
A 2335.4
(1)
P 		2805.8
(2.3)
P 		3085.3
(3)
P 		2597.3
(1.7)
P 		1086.9
(-2.3)
P 		804.5
(-3)
P 		2281
B 1909
(-0.2)
P 		2641
(1.6)
P 		3227
(3)
P 		1896.8
(-0.2)
P 		1028.7
(-2.3)
P 		732
(-3)
P 		2495
C 1778
(-0.9)
P 		2466.8
(0.7)
P 		3474.3
(3)
P 		2792.8
(1.4)
P 		1123.9
(-2.4)
P 		861.3
(-3)
P 		2613
avr 2007.5
(-0.1) 		2637.9
(1.5) 		3262.2
(3) 		2429
(1) 		1079.8
(-2.3) 		799.3
(-3) 		2463
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 686
1 2 3 4 range
A 744.1
(3)
P 		334.3
(-0.6)
P 		555.5
(1.3)
P 		58.6
(-3)
P 		686
B 631.5
(3)
P 		417.5
(0.7)
P 		487.5
(1.4)
P 		80.3
(-3)
P 		551
C 613.7
(2.3)
P 		451.2
(0.7)
P 		690.5
(3)
P 		67.2
(-3)
P 		623
avr 663.1
(3) 		401
(0.4) 		577.8
(2.1) 		68.7
(-3) 		594

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 802
1 2 3 4 5 6 range
A 430.5
(-1.2)
P 		997.9
(3)
P 		671.9
(0.6)
P 		658.7
(0.5)
P 		328
(-2)
P 		195.9
(-3)
P 		802
B 664.2
(0.9)
P 		879.1
(3)
P 		783.6
(2.1)
P 		502.4
(-0.7)
P 		418
(-1.6)
P 		273
(-3)
P 		606
C 659
(0.7)
P 		752.7
(1.7)
P 		884.3
(3)
P 		561.6
(-0.2)
P 		363.7
(-2.2)
P 		288.3
(-3)
P 		596
avr 584.6
(0.2) 		876.6
(3) 		779.9
(2.1) 		574.2
(0.1) 		369.9
(-1.9) 		252.4
(-3) 		624
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191404_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11563
Sequence Type Exemplar sequence
Representative Public ID F43H9.2 NCBI
Target Description F43H9.2 /REP_DB=WormBase Gene ID /WP=CE07246 /CHR=5 /FEA=Sanger Annotation /DEF=serine palmtoyltransferase (ST.LOUIS) TR:Q20375 protein_id:AAA92303.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072663(11), NM_072664(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072663 NCBI Caenorhabditis elegans Serine Palmitoyl Transferase famiLy family member (sptl-2) (sptl-2) mRNA, complete cds. 11/11 None
NM_072664 NCBI Caenorhabditis elegans Serine Palmitoyl Transferase famiLy family member (sptl-2) (sptl-2) mRNA, complete cds. 11/11 None
SNAP00000036916 ENSEMBL cdna:SNAP chromosome:CEL140:V:8021755:8024164:1 11/11 None
GENEFINDER00000036921 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8020538:8024164:1 11/11 None
F43H9.2b ENSEMBL cdna:known chromosome:CEL140:V:8020538:8024303:1 gene:F43H9.2 11/11 None
F43H9.2a ENSEMBL cdna:known chromosome:CEL140:V:8021729:8024164:1 gene:F43H9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8021755-8024165(+) 100.0 100.0
Public Domain and Genome References
Ensembl F43H9.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK71365 Serine palmitoyl transferase family protein 2, isoform b [Caenorhabditis elegans] ref|NP_505064.1| Serine Palmitoyl Transferase famiLy family member (sptl-2) [Caenorhabditis elegans] 0.0
blast AAA92303 Serine palmitoyl transferase family protein 2, isoform a [Caenorhabditis elegans] ref|NP_505065.1| Serine Palmitoyl Transferase famiLy family member (sptl-2) [Caenorhabditis elegans] 0.0
blast CAE66157 Hypothetical protein CBG11388 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 2.6E-24
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 2.6E-24
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 2.6E-24
Sequence
Target Sequence 
>C. ELEGANS:191404_AT
aaagcgtactgtagaccatcttcgtgctgcaagtccaactggatactattcctctccaat
gtctccaccaattgctcaacaaatctatacttccatgtctattattatgggcaaagatgg
aacaaaggatggtgctcagagaatcgaaagactcgcaagaaactctcattacttccgaat
gaaattgaaacaaaatggatttattgtctatggaagtaatgattctccagtcgttccaat
gctcatctatttcccaacaatgtgtggattctatggtcgtgaaatgttggcaagaaatat
tggatgtgttgttgtatcattcccagctactcatatgacagaaggaagagttcgattctg
tatttcggccgctcacaccaaagagcaacttgatgaggtcctcgaaacagtcaaccttct
tggatcgatgagtcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGCGTACTGTAGACCATCTTCGT 69 125 1200 Antisense
CATCTTCGTGCTGCAAGTCCAACTG 189 213 1216 Antisense
GTCCAACTGGATACTATTCCTCTCC 623 477 1232 Antisense
GCAAGAAACTCTCATTACTTCCGAA 385 317 1354 Antisense
AAGTAATGATTCTCCAGTCGTTCCA 575 155 1413 Antisense
AATGCTCATCTATTTCCCAACAATG 517 165 1437 Antisense
TGTGTTGTTGTATCATTCCCAGCTA 481 555 1504 Antisense
AGTTCGATTCTGTATTTCGGCCGCT 53 65 1548 Antisense
TTCGGCCGCTCACACCAAAGAGCAA 312 695 1563 Antisense
GGTCCTCGAAACAGTCAACCTTCTT 336 497 1596 Antisense
CAACCTTCTTGGATCGATGAGTCGA 234 187 1611 Antisense
Sequence Source Affymetrix Proprietary Database