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[191394_s_at] metalloprotease

Description F40E10.1 /REP_DB=WormBase Gene ID /WP=CE24964 /GEN=hch-1 /TR=Q21059 /GB=CAB61002.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=metalloprotease
Cluster 6grp_total > 68      4grp_total > 18      6grp_cgc > 38      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 3.7
(-3)
A 		62.1
(-0.1)
A 		16.4
(-2.4)
A 		122.6
(3)
A 		119
B 16.5
(-1.7)
A 		13.1
(-2)
A 		4.7
(-3)
A 		57.6
(3)
A 		53
C 11.3
(-3)
A 		24.4
(-1.9)
A 		20.3
(-2.2)
A 		79.7
(3)
A 		68
avr 10.5
(-3) 		33.2
(-1.2) 		13.8
(-2.7) 		86.6
(3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 664
1 2 3 4 5 6 range
A 280.9
(-0.8)
P 		53.8
(-3)
A 		55
(-3)
A 		101.9
(-2.5)
A 		117.1
(-2.4)
A 		668.7
(3)
P 		615
B 198.8
(-1.1)
P 		40
(-2.7)
A 		12.4
(-3)
A 		117.5
(-1.9)
A 		137.9
(-1.7)
A 		587.2
(3)
P 		575
C 174.3
(-1.4)
P 		47
(-2.6)
A 		4.9
(-3)
A 		128.6
(-1.8)
A 		131.4
(-1.8)
A 		648
(3)
P 		643
avr 218
(-1.1) 		46.9
(-2.8) 		24.1
(-3) 		116
(-2.1) 		128.8
(-2) 		634.6
(3) 		611
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 1.3
(-3)
A 		13.5
(1.4)
A 		4.3
(-1.9)
A 		17.9
(3)
A 		17
B 5.3
(-0.8)
A 		3.2
(-1.9)
A 		1.1
(-3)
A 		12.3
(3)
A 		11
C 3.7
(-3)
A 		7
(-1)
A 		7.1
(-0.9)
A 		13.4
(3)
A 		10
avr 3.4
(-3) 		7.9
(-0.6) 		4.2
(-2.6) 		14.5
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 51.8
(-1.4)
P 		19.1
(-2.7)
A 		12
(-3)
A 		25.8
(-2.5)
A 		35.3
(-2.1)
A 		162.8
(3)
P 		151
B 69.2
(-1.2)
P 		13.3
(-2.7)
A 		3
(-3)
A 		31.1
(-2.2)
A 		56
(-1.5)
A 		219
(3)
P 		216
C 64.6
(-1.2)
P 		14.3
(-2.6)
A 		1.3
(-3)
A 		25.9
(-2.3)
A 		42.5
(-1.9)
A 		216.9
(3)
P 		216
avr 61.9
(-1.3) 		15.6
(-2.7) 		5.4
(-3) 		27.6
(-2.3) 		44.6
(-1.8) 		199.6
(3) 		194
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191394_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17324
Sequence Type Exemplar sequence
Representative Public ID F40E10.1 NCBI
Target Description F40E10.1 /REP_DB=WormBase Gene ID /WP=CE24964 /GEN=hch-1 /TR=Q21059 /GB=CAB61002.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078039(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078039 NCBI Caenorhabditis elegans F40E10.1 (metalloprotease) mRNA, complete cds. 11/11 None
SNAP00000014850 ENSEMBL cdna:SNAP chromosome:CEL140:X:14696437:14699883:-1 11/11 None
GENEFINDER00000014855 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14696437:14699883:-1 11/11 None
F40E10.1 ENSEMBL cdna:known chromosome:CEL140:X:14696330:14699883:-1 gene:F40E10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14696436-14699883(-) 100.0 100.0
Public Domain and Genome References
Gene Title metalloprotease
Gene Symbol hch-1
Ensembl F40E10.1
Entrez Gene 181564 Entrez gene
SwissProt Q21059 EMBL-EBI
WormBase CE24964 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61002 Hypothetical protein F40E10.1 [Caenorhabditis elegans] ref|NP_510440.1| F40E10.1 [Caenorhabditis elegans] sp|Q21059|NAS34_CAEEL Zinc metalloproteinase nas-34 precursor (Nematode astacin 34) (Defective hatching protein 1) dbj|BAA12861.1| HCH-1 [Caenorhabditis elegans] 0.0
blast CAE63216 Hypothetical protein CBG07573 [Caenorhabditis briggsae] 0.0
blast T22021 hypothetical protein F40E10.1 - Caenorhabditis elegans 1.0E-175
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.5E-105
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.4E-105
Sequence
Target Sequence 
>C. ELEGANS:191394_S_AT
tctacttccaaatgacagctgccacattctcccgatactctccatgcaccaccaactacc
tggaaatcaactacggaagagatttcagcagagttggagcacgattctgtgcatcttatc
caactatctccttatctgaaaccaatactcttgttgtgatttataaaggagtaaacggag
cgaggttctcattgaactatcgttatgatcctgtaactttttcaacatctgccccaacaa
ccacttccaccaccacgaccacggctccaataacggttccaaccgtttccccaactacaa
ctactaccaggcaaaccacaactaccgctcgcacatctacaacaaccaccactacccaag
ctccacctacgaccaccacgtcaacatcgcaatgtgcatcctggtcggcttgttccgcac
aatgtggaggatgtggaactcaatcgagaagatgcggtacctacgtggaaactgtctact
gtaacaccaacccatgtactggaggatattgctgccgtccgttcttctacgtaacatcgt
tcggcactggatactgtagacggccaggagcagatacaccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTACTTCCAAATGACAGCTGCCAC 146 615 1211 Antisense
ATGCACCACCAACTACCTGGAAATC 629 43 1254 Antisense
CTTATCCAACTATCTCCTTATCTGA 44 223 1325 Antisense
TACTACCAGGCAAACCACAACTACC 7 647 1512 Antisense
ACATCGCAATGTGCATCCTGGTCGG 280 105 1594 Antisense
GCGGTACCTACGTGGAAACTGTCTA 486 239 1664 Antisense
AACTGTCTACTGTAACACCAACCCA 143 143 1680 Antisense
ACTGGAGGATATTGCTGCCGTCCGT 375 97 1708 Antisense
GTCCGTTCTTCTACGTAACATCGTT 107 477 1727 Antisense
AACATCGTTCGGCACTGGATACTGT 71 137 1743 Antisense
AGACGGCCAGGAGCAGATACACCAG 2 71 1768 Antisense
Sequence Source Affymetrix Proprietary Database