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[191393_s_at] glutathione S-transferase

Description Y53F4B.30 /REP_DB=WormBase Gene ID /WP=CE22417 /TR=Q9NAB3 /GB=CAB61104.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 41      4grp_highest_8_8 > 24      4grp_high_8_8 > 60      6grp_highest_nonorm > 34      6grp_total > 31      4grp_total > 60      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4734
1 2 3 4 range
A 4385.7
(2.2)
P 		4394.3
(2.2)
P 		4999.8
(3)
P 		266
(-3)
P 		4734
B 4178.8
(2)
P 		4848.5
(2.9)
P 		4961.9
(3)
P 		292.9
(-3)
P 		4669
C 4260.7
(2.5)
P 		4609.9
(3)
P 		4440.4
(2.8)
P 		333.3
(-3)
P 		4277
avr 4275.1
(2.3) 		4617.6
(2.8) 		4800.7
(3) 		297.4
(-3) 		4503

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3903
1 2 3 4 5 6 range
A 2205.7
(-0)
P 		3307.5
(2)
P 		3821.1
(3)
P 		2938.6
(1.3)
P 		1431.3
(-1.5)
P 		619.6
(-3)
P 		3202
B 2450.8
(0.9)
P 		2938.9
(2)
P 		3405.4
(3)
P 		2406.2
(0.8)
P 		1725.8
(-0.6)
P 		625.5
(-3)
P 		2780
C 2830.9
(0.3)
P 		2901
(0.4)
P 		4522.5
(3)
P 		2274.5
(-0.6)
P 		1960.6
(-1.1)
P 		760.1
(-3)
P 		3762
avr 2495.8
(0.4) 		3049.1
(1.4) 		3916.3
(3) 		2539.8
(0.5) 		1705.9
(-1.1) 		668.4
(-3) 		3248
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1558
1 2 3 4 range
A 1596.8
(3)
P 		953.9
(0.5)
P 		1307
(1.9)
P 		38.9
(-3)
P 		1558
B 1332.6
(3)
P 		1197.5
(2.4)
P 		1149.8
(2.1)
P 		62.4
(-3)
P 		1270
C 1410.4
(2.4)
P 		1325.4
(2.1)
P 		1547.6
(3)
P 		56.2
(-3)
P 		1491
avr 1446.6
(3) 		1158.9
(1.8) 		1334.8
(2.5) 		52.5
(-3) 		1394

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1026
1 2 3 4 5 6 range
A 406.6
(-1.5)
P 		1176.3
(3)
P 		832.2
(1)
P 		745.3
(0.5)
P 		431.9
(-1.4)
P 		150.8
(-3)
P 		1026
B 852.8
(2)
P 		978.2
(3)
P 		826.9
(1.8)
P 		637.3
(0.3)
P 		701.2
(0.8)
P 		233.3
(-3)
P 		745
C 1049.3
(2.3)
P 		885.2
(1.2)
P 		1151.1
(3)
P 		457.4
(-1.6)
P 		634.4
(-0.5)
P 		254.5
(-3)
P 		897
avr 769.6
(1.2) 		1013.2
(3) 		936.7
(2.4) 		613.3
(0) 		589.2
(-0.2) 		212.9
(-3) 		800
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191393_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2685
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.30 NCBI
Target Description Y53F4B.30 /REP_DB=WormBase Gene ID /WP=CE22417 /TR=Q9NAB3 /GB=CAB61104.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064715(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064715 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-27) (gst-27) mRNA, complete cds. 11/11 None
SNAP00000043068 ENSEMBL cdna:SNAP chromosome:CEL140:II:15164830:15165559:-1 11/11 None
GENEFINDER00000043138 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15164830:15165559:-1 11/11 None
Y53F4B.30 ENSEMBL cdna:known chromosome:CEL140:II:15164791:15165616:-1 gene:Y53F4B.30 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15164826-15165556(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-27
Ensembl Y53F4B.30
Entrez Gene 175166 Entrez gene
SwissProt Q9NAB3 EMBL-EBI
WormBase CE22417 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61104 Hypothetical protein Y53F4B.30 [Caenorhabditis elegans] ref|NP_497116.1| Glutathione S-Transferase family member (gst-27) [Caenorhabditis elegans] 1.0E-116
blast CAB61103 Hypothetical protein Y53F4B.29 [Caenorhabditis elegans] ref|NP_497115.1| Glutathione S-Transferase family member (gst-26) [Caenorhabditis elegans] 3.0E-98
blast CAE73239 Hypothetical protein CBG20648 [Caenorhabditis briggsae] 1.0E-83
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 6.9E-11
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 3.9E-15
Sequence
Target Sequence 
>C. ELEGANS:191393_S_AT
aagctgacctattttaacgtgcgtggatatggagaaccagctcgtattctgttccatctt
gccgatgttccatttgaagatttccgaatgaccattggagatggaacatgggaaaatctc
aaagccaaaactccatttggacaagccccagtcttatcagttgatggatttgaaattcca
caatccgctgcaatcaaccgctaccttgccaagcaattcggatatgctggaaaaaccccg
gaggagcaagcctggactgatgctatcgtcgatcaatacaaggattttatggtatcaatc
aaagaagtgggaaaagcaagcgctgctggaaagtctgccgaggaggtcgggaagatcatc
cagtcagatctcgttccggcaagagatgcattctttgtgataatcaacaagatccttgag
aagagcaagtccggatttttagttggagatggtctaaccatcgcagatattgtgattgtg
gaatgcatcacaactttggacaaacatcagctcttcactgcatcggagcaaccaaaattg
gtcgcgcttcgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCTGACCTATTTTAACGTGCGTG 489 149 25 Antisense
GAGAACCAGCTCGTATTCTGTTCCA 647 391 56 Antisense
ACAAGCCCCAGTCTTATCAGTTGAT 109 113 165 Antisense
CCCGGAGGAGCAAGCCTGGACTGAT 298 207 261 Antisense
GCCTGGACTGATGCTATCGTCGATC 362 283 274 Antisense
CAAGCGCTGCTGGAAAGTCTGCCGA 640 185 341 Antisense
GATCATCCAGTCAGATCTCGTTCCG 42 421 378 Antisense
ATGGTCTAACCATCGCAGATATTGT 106 51 473 Antisense
TTTGGACAAACATCAGCTCTTCACT 289 667 519 Antisense
CTCTTCACTGCATCGGAGCAACCAA 21 231 535 Antisense
AACCAAAATTGGTCGCGCTTCGAGA 87 145 554 Antisense
Sequence Source Affymetrix Proprietary Database