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[191385_s_at]

Description H42K12.1 /REP_DB=WormBase Gene ID /WP=CE27386 /GEN=pdk-1 /TR=O17349 /GB=AAB71265.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_high1_10_10 > 0      6grp_total > 09      4grp_total > 53      6grp_cgc > 10      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 116.2
(3)
P
69.7
(-0.1)
A
102.1
(2.1)
A
26.3
(-3)
A
90
B 92
(3)
P
79.8
(2.1)
A
36.8
(-1.2)
A
13.9
(-3)
A
78
C 99.4
(1.9)
P
108.4
(3)
P
69.4
(-1.6)
A
57.6
(-3)
A
51
avr 102.5
(3)
86
(1.6)
69.4
(0.2)
32.6
(-3)
70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 134.5
(2.8)
P
95.5
(-1.5)
P
85.7
(-2.6)
P
136.1
(3)
P
84.5
(-2.8)
P
82.4
(-3)
P
54
B 80
(-0.5)
P
109.7
(3)
P
107.8
(2.8)
P
60
(-2.8)
A
58.6
(-3)
P
58.2
(-3)
P
52
C 180.6
(3)
P
43.7
(-3)
P
118.7
(0.3)
M
110.9
(-0.1)
P
99.3
(-0.6)
P
59.4
(-2.3)
P
137
avr 131.7
(3)
83
(-1.5)
104.1
(0.5)
102.3
(0.3)
80.8
(-1.7)
66.7
(-3)
65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 42.3
(3)
P
15.1
(-1.2)
A
26.7
(0.6)
A
3.8
(-3)
A
39
B 29.3
(3)
P
19.7
(0.8)
A
8.5
(-1.7)
A
3
(-3)
A
26
C 32.9
(3)
P
31.2
(2.6)
P
24.2
(0.8)
A
9.7
(-3)
A
23
avr 34.8
(3)
22
(0.4)
19.8
(-0.1)
5.5
(-3)
29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 24.8
(-0.7)
P
34
(2.8)
P
18.7
(-3)
P
34.5
(3)
P
25.5
(-0.4)
P
20.1
(-2.5)
P
16
B 27.8
(0.5)
P
36.5
(3)
P
26.2
(-0)
P
15.9
(-3)
A
23.8
(-0.7)
P
21.7
(-1.3)
P
21
C 66.9
(3)
P
13.3
(-3)
P
30.2
(-1.1)
M
22.3
(-2)
P
32.1
(-0.9)
P
19.9
(-2.3)
P
54
avr 39.8
(3)
27.9
(-0.7)
25
(-1.6)
24.2
(-1.9)
27.1
(-1)
20.6
(-3)
19
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191385_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18104
Sequence Type Exemplar sequence
Representative Public ID H42K12.1 NCBI
Target Description H42K12.1 /REP_DB=WormBase Gene ID /WP=CE27386 /GEN=pdk-1 /TR=O17349 /GB=AAB71265.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029572(11), NM_001029571(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_001029572 NCBI Caenorhabditis elegans PDK-class protein kinase family member (pdk-1) (pdk-1) mRNA, complete cds. 11/11 None
NM_001029571 NCBI Caenorhabditis elegans PDK-class protein kinase family member (pdk-1) (pdk-1) mRNA, complete cds. 11/11 None
SNAP00000041173 ENSEMBL cdna:SNAP chromosome:CEL140:X:1324462:1327488:1 11/11 None
GENEFINDER00000041174 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:1324462:1327488:1 11/11 None
H42K12.1b ENSEMBL cdna:known chromosome:CEL140:X:1321348:1327980:1 gene:H42K12.1 11/11 A 
H42K12.1a ENSEMBL cdna:known chromosome:CEL140:X:1321348:1327980:1 gene:H42K12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1323814-1325607(+) 100.0 100.0
Public Domain and Genome References
Ensembl H42K12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK82905 Pdk-class protein kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024743.1| PDK-class protein kinase family member (pdk-1) [Caenorhabditis elegans] gb|AAD42307.1| phophoinositide-dependent protein kinase 1a [Caenorhabditis elegans] 0.0
blast AAK82904 Pdk-class protein kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024742.1| PDK-class protein kinase family member (pdk-1) [Caenorhabditis elegans] gb|AAD42308.1| phophoinositide-dependent protein kinase 1b [Caenorhabditis elegans] 0.0
blast CAE63571 Hypothetical protein CBG08059 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-16
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-16
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 6.0E-65
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 1.0E-65
hanks 1.2.6 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKCe 8.0E-31
hanks 1.2.6 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; PKCe 7.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.2E-68
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-68
Pfam IPR000719 EMBL-EBI Protein kinase 5.3E-56
Sequence
Target Sequence
>C. ELEGANS:191385_S_AT
aatggtgatcttggcgagtcgctgtgccattttggatcattcgacatgctcacctcaaaa
ttctttgcctcggaaatcctcaccggactgcaattcctacacgacaacaaaattgtgcac
agagacatgaagccggacaatgtgctcatccagaaagacgaagagaacactgctcgacgt
accacatttgttggaactgctctctacgtgagcccggagatgctagctgacggagatgtg
ggaccacaaaccgacatttggggattgggatgtatccttttccagtgtctagccggacag
ccaccattcagagccgtcaaccagtaccatcttttgaaaagaatccaggagttggatttc
tcgttcccagaaggatttccagaggaagcgtcggaaattatcgcaaagattttggtgcgc
gacccgagtacccgtatcaccagtcaagaacttatggctcacaagttttttgaaaacgtt
gactgggtgaacattgcaaatatcaagccaccagtcctgcacgcctacattccagccaca
tttggc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
AATGGTGATCTTGGCGAGTCGCTGT 353 163 352 Antisense
GAGTCGCTGTGCCATTTTGGATCAT 246 401 367 Antisense
TTTGGATCATTCGACATGCTCACCT 219 667 382 Antisense
GCTCACCTCAAAATTCTTTGCCTCG 465 301 399 Antisense
CGGACTGCAATTCCTACACGACAAC 660 241 435 Antisense
GAAGCCGGACAATGTGCTCATCCAG 191 345 480 Antisense
TTGGAACTGCTCTCTACGTGAGCCC 679 707 542 Antisense
CGTGAGCCCGGAGATGCTAGCTGAC 270 245 558 Antisense
GGATGTATCCTTTTCCAGTGTCTAG 217 515 619 Antisense
CCAGGAGTTGGATTTCTCGTTCCCA 123 273 696 Antisense
CGCCTACATTCCAGCCACATTTGGC 106 255 873 Antisense
Sequence Source Affymetrix Proprietary Database